15 research outputs found

    MiRNA expression plots and predicted secondary structures.

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    <p>Different colours in the plot correspond to different tissues as described in C). Green and purple-labelled bases in the hairpin figure indicate the most abundant read mapping to the 5' and 3' ends; conserved miRNA cfa-miR-371 (A) appears to be absent in heart and shows variable expression levels in the other tissues. B) Plots for a novel miRNA located in chromosome 15, supported by small RNA reads from the brain sample.</p

    Workflow for the generation of miRNA expression profiles and secondary structures.

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    <p>Small RNA reads are first mapped (A) to the hairpin sequences, looking for two peak alignment patterns as shown in B (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0153453#pone.0153453.s004" target="_blank">S4</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0153453#pone.0153453.s005" target="_blank">S5</a> Figs). Expression levels across each hairpin (B) and secondary structures (C) are then derived (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0153453#pone.0153453.s006" target="_blank">S6</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0153453#pone.0153453.s007" target="_blank">S7</a> Figs).</p
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