Location of study area.

<p>The location of the municipality of São João das Missões in northern Minas Gerais, Brazil. The native village of Imbaúbas located in the Xakriabá Indigenous Reserve, where the study was performed, is indicated.</p

Species identification of <i>Leishmania</i> from sand flies collected from trails in the Xakriabá Indigenous Reserve, Brazil, during the study period.

<p>Sand flies were grouped in pools of up to ten specimens of the same species, locality, and date, and ITS1 PCR-RFLP was performed with total DNA extracted from these pools. The figure represents an ethidium bromide-stained 4% agarose gel in which the amplicons were submitted to electrophoresis. Lanes: MW, molecular weight marker—100 bp; lanes 1–4, positive controls of <i>Le</i>. <i>amazonensis</i> (IFLA/BR/67/PH8), <i>Le</i>. <i>braziliensis</i> (MHOM/BR/75/M2903), <i>Le</i>. <i>infantum</i> (MHOM/BR/74/PP75), <i>Le</i>. <i>guyanensis</i> (MHOM/BR/75/M4147) respectively; 5–14, phlebotomine positive pools.</p

Detection of <i>Leishmania</i> in sand flies collected from trails in the Xakriabá Indigenous Reserve, Brazil, during the study period.

<p>Sand flies were pooled with up to ten specimens of the same locality, species and date, and ITS1 PCR was performed with total DNA extracted from these pools. The figure represents an ethidium bromide-stained 2% agarose gel in which the amplicons were submitted to electrophoresis. Lanes: MW, molecular weight marker—100 bp; lanes 1–4, positive controls of <i>Le</i>. <i>amazonensis</i> (IFLA/BR/67/PH8), <i>Le</i>. <i>braziliensis</i> (MHOM/BR/75/M2903), <i>Le</i>. <i>infantum</i> (MHOM/BR/74/PP75), <i>Le</i>. <i>guyanensis</i> (MHOM/BR/75/M4147) respectively; 5–14, phlebotomine positive pools; NC, negative control.</p

Environmental characteristics of the sand fly collection sites in PES from September 2011 to August 2013.

<p>Environmental characteristics of the sand fly collection sites in PES from September 2011 to August 2013.</p

Species accumulation curve, abundance, and species richness of the sand fly fauna of PES from September 2011 to August 2013.

<p>Species accumulation curve, abundance, and species richness of the sand fly fauna of PES from September 2011 to August 2013.</p

Environmental characteristics of the sand fly collection sites in PES from September 2011 to August 2013.

<p>Environmental characteristics of the sand fly collection sites in PES from September 2011 to August 2013.</p

Map showing the location of PES and the location of sampling sites in the park.

<p>(1)—Park surroundings—peridomestic areas; (2)—Cerrado 1; (3)—Cerrado 2; (4)—Rupicolous vegetation; (5)—Cave entrance; (6)—Cave surroundings.</p