Testing use of mitochondrial COI sequences for the identification and phylogenetic analysis of New Zealand caddisflies (Trichoptera)

We tested the hypothesis that cytochrome c oxidase subunit 1 (COI) sequences would successfully discriminate recognised species of New Zealand caddisflies. We further examined whether phylogenetic analyses, based on the COI locus, could recover currently recognised superfamilies and suborders. COI sequences were obtained from 105 individuals representing 61 species and all 16 families of Trichoptera known from New Zealand. No sequence sharing was observed between members of different species, and congeneric species showed from 2.3 to 19.5% divergence. Sequence divergence among members of a species was typically low (mean = 0.7%; range 0.0–8.5%), but two species showed intraspecific divergences in excess of 2%. Phylogenetic reconstructions based on COI were largely congruent with previous conclusions based on morphology, although the sequence data did not support placement of the purse-cased caddisflies (Hydroptilidae) within the uncased caddisflies, and, in particular, the Rhyacophiloidea. We conclude that sequence variation in the COI gene locus is an effective tool for the identification of New Zealand caddisfly species, and can provide preliminary phylogenetic inferences. Further research is needed to ascertain the significance of the few instances of high intra-specific divergence and to determine if any instances of sequence sharing will be detected with larger sample sizes

The Effects of Repetitive Drop Jumps on Impact Phase Joint Kinematics and Kinetics

The purpose of the study was to investigate the effects of fatigue on lower extremity joint kinematics, and kinetics during repetitive drop jumps. Twelve recreationally active males (n = 6) and females (n = 6) (nine used for analysis) performed repetitive drop jumps until they could no longer reach 80% of their initial drop jump height. Kinematic and kinetic variables were assessed during the impact phase (100 ms) of all jumps. Fatigued landings were performed with increased knee extension, and ankle plantar flexion at initial contact, as well as increased ankle range of motion during the impact phase. Fatigue also resulted in increased peak ankle power absorption and increased energy absorption at the ankle. This was accompanied by an approximately equal reduction in energy absorption at the knee. While the knee extensors were the muscle group primarily responsible for absorbing the impact, individuals compensated for increased knee extension when fatigued by an increased use of the ankle plantar flexors to help absorb the forces during impact. Thus, as fatigue set in and individuals landed with more extended lower extremities, they adopted a landing strategy that shifted a greater burden to the ankle for absorbing the kinetic energy of the impact

The Observed Trend of Boron and Oxygen in Field Stars of the Disk

Oxygen abundances are derived in a sample of 13 field F and G dwarfs and subgiants with metallicities in the range of -0.75 < [Fe/H] < +0.15. This is the same sample of stars for which boron abundances have been derived earlier from archived spectra obtained with the Hubble Space Telescope. In a log-log comparison of the B versus the O abundances, a slope of m(BO)=1.39 is found, indicating that in the disk, the abundance of B relative to O is intermediate between primary and secondary production (hybrid behavior). This relation of B versus O for disk stars is compared to the same relation for halo stars.Comment: 14 pages, 3 figures, 1 table. In press to The Astronomical Journal (July 2001

Amphibian Immune Defenses against Chytridiomycosis: Impacts of Changing Environments

Eco-immunology is the field of study that attempts to understand the functions of the immune system in the context of the host's environment. Amphibians are currently suffering devastating declines and extinctions in nearly all parts of the world due to the emerging infectious disease chytridiomycosis caused by the chytrid fungus, Batrachochytrium dendrobatidis. Because chytridiomycosis is a skin infection and remains confined to the skin, immune defenses of the skin are critical for survival. Skin defenses include secreted antimicrobial peptides and immunoglobulins as well as antifungal metabolites produced by symbiotic skin bacteria. Low temperatures, toxic chemicals, and stress inhibit the immune system and may impair natural defenses against B. dendrobatidis. Tadpoles' mouth parts can be infected by B. dendrobatidis. Damage to the mouth parts can impair growth, and the affected tadpoles maintain the pathogen in the environment even when adults have dispersed. Newly metamorphosing frogs appear to be especially vulnerable to infection and to the lethal effects of this pathogen because the immune system undergoes a dramatic reorganization at metamorphosis, and postmetamorphic defenses are not yet mature. Here we review our current understanding of amphibian immune defenses against B. dendrobatidis and the ability of the pathogen to resist those defenses. We also briefly review what is known about the impacts of temperature, environmental chemicals, and stress on the host-pathogen interactions and suggest future directions for researc

Stellar intensity interferometry: Experimental steps toward long-baseline observations

Experiments are in progress to prepare for intensity interferometry with arrays of air Cherenkov telescopes. At the Bonneville Seabase site, near Salt Lake City, a testbed observatory has been set up with two 3-m air Cherenkov telescopes on a 23-m baseline. Cameras are being constructed, with control electronics for either off- or online analysis of the data. At the Lund Observatory (Sweden), in Technion (Israel) and at the University of Utah (USA), laboratory intensity interferometers simulating stellar observations have been set up and experiments are in progress, using various analog and digital correlators, reaching 1.4 ns time resolution, to analyze signals from pairs of laboratory telescopes.Comment: 12 pages, 3 figur

A three dimensional kinetic model for the formation of acrylamide in french fries with variable glucose and fructose content

For modeling and predicting the formation of acrylamide in real food systems, it is not only important to understand the underlying chemistry, but also the complex effects of heat and moisture transfer which means that in French fries, for example, most of the acrylamide is formed on the edges and corners of the fry. In this paper, simple approximations have been employed to build a mathematical model that approximates the heat and mass transport phenomena in three dimensions. It successfully predicts the concentration of acrylamide and its precursors at different positions in the ‘fry’

A Search for OH Megamasers at z > 0.1. I. Preliminary Results

We present the preliminary results of a survey for OH megamasers underway at the Arecibo Observatory. The goals of the survey are to calibrate the luminosity function of OH megamasers to the low-redshift galaxy merger rate (0.1 < z < 0.2), and to use the enhanced sample of OH megamasers provided by the survey to study OH megamaser environments, engines, lifetimes, and structure. The survey should double the known OH megamaser sample to roughly 100 objects. Survey results will be presented in installments to facilitate community access to the data. Here we report the discovery of 11 OH megamasers and one OH absorber, and include upper limits on the isotropic 1667 MHz OH line luminosity of 53 other luminous infrared galaxies at z > 0.1. The new megamasers show a wide range of spectral properties, but are consistent with the extant set of 55 previously reported objects, only 8 of which have z > 0.1.Comment: 18 pages, 3 figures, accepted for publication in the Astronomical Journa