MiR-9 Controls Chemotactic Activity of Cord Blood CD34⁺ Cells by Repressing CXCR4 Expression

Improved approaches for promoting umbilical cord blood (CB) hematopoietic stem cell (HSC) homing are clinically important to enhance engraftment of CB-HSCs. Clinical transplantation of CB-HSCs is used to treat a wide range of disorders. However, an improved understanding of HSC chemotaxis is needed for facilitation of the engraftment process. We found that ectopic overexpression of miR-9 and antisense-miR-9 respectively down- and up-regulated C-X-C chemokine receptor type 4 (CXCR4) expression in CB-CD34＋ cells as well as in 293T and TF-1 cell lines. Since CXCR4 is a specific receptor for the stromal cell derived factor-1 (SDF-1) chemotactic factor, we investigated whether sense miR-9 and antisense miR-9 influenced CXCR4-mediated chemotactic mobility of primary CB CD34＋ cells and TF-1 cells. Ectopic overexpression of sense miR-9 and antisense miR-9 respectively down- and up-regulated SDF-1-mediated chemotactic cell mobility. To our knowledge, this study is the first to report that miR-9 may play a role in regulating CXCR4 expression and SDF-1-mediated chemotactic activity of CB CD34＋ cells

Npas4 regulates IQSEC3 expression in hippocampal somatostatin interneurons to mediate anxiety-like behavior

Activity-dependent GABAergic synapse plasticity is important for normal brain functions, but the underlying molecular mechanisms remain incompletely understood. Here, we show that Npas4 (neuronal PAS-domain protein 4) transcriptionally regulates the expression of IQSEC3, a GABAergic synapse-specific guanine nucleotide-exchange factor for ADP-ribosylation factor (ARF-GEF) that directly interacts with gephyrin. Neuronal activation by an enriched environment induces Npas4-mediated upregulation of IQSEC3 protein specifically in CA1 stratum oriens layer somatostatin (SST)-expressing GABAergic interneurons. SST+ interneuron-specific knockout (KO) of Npas4 compromises synaptic transmission in these GABAergic interneurons, increases neuronal activity in CA1 pyramidal neurons, and reduces anxiety behavior, all of which are normalized by the expression of wild-type IQSEC3, but not a dominant-negative ARF-GEF-inactive mutant, in SST+ interneurons of Npas4-KO mice. Our results suggest that IQSEC3 is a key GABAergic synapse component that is directed by Npas4 and ARF activity, specifically in SST+ interneurons, to orchestrate excitation-to-inhibition balance and control anxiety-like behavior.1

GIS Based Assessment and Design for Areas Vulnerable to Soil Disasters: Case Study of Namhyeun-dong, South Korea

Due to climate change, heavy rainfall events that trigger landslips are becoming common. This study investigates patterns in the soil’s response to major rainfall events in mountainous areas and proposes a new approach for resilient disaster prevention technology and recovery based on the effect of soil runoff. Namhyeon-dong within Seoul was selected for the case study because of its vulnerable location between two mountains. A master plan was developed to cope with the predicted soil runoff based on the annual rainfall, local land use and a series of 10-year forecasts covering the period from 2021 to 2100. A total of 22 catchments in the study area were analyzed with Arc Hydro, an ArcGIS plug-in and appropriate technologies proposed to deal with the soil runoff likely to be experienced in each catchment in an extreme disaster. The resulting model was deemed adequate to deal with disasters during the period predicted to represent the highest risk, 2051–2060. The study’s findings will help to forecast disasters from flood that could impact residential areas in mountainous regions, to predict the magnitude of potential soil disasters in individual regions and develop design guidelines for disaster prevention technology based on the predicted amount of soil runoff

Pilot-Scale Bio-Augmented Aerobic Composting of Excavated Foot-And-Mouth Disease Carcasses

In the present work, we tested the validity of using novel, bio-augmented, aerobic composting with carcass-degrading microorganisms for the ex situ stabilization of carcasses at pilot scale with previously poorly decomposed carcasses excavated from a 3-year old burial site. The significantly decreased chemical oxygen demand (COD, 160,000 mg/kg to 40,000 mg/kg) and inorganic nitrogen species (total nitrogen, 5000 mg/kg to 2000 mg/kg) indicated effective bio-stabilization of carcasses by bio-augmented composting. The subsequent germination assays and the quantitative characterization of potentially pathogenic bacteria using NGS (next-generation sequencing) showed that the burial–composting sequential system with the carcass-degrading microorganisms and mechanical agitation successfully reduced plant toxicity as well as microbial risk to human health, suggesting that the composting by-product is suitable for farming or/and landfill use(s)

In Vivo and In Vitro Anticancer Activity of Doxorubicin-loaded DNA-AuNP Nanocarrier for the Ovarian Cancer Treatment

In this study, we have determined the anticancer activity of doxorubicin (Dox)-loaded DNA/gold nanoparticle (AuNP) nanocarrier (Dox-DNA-AuNP) for the treatment of ovarian cancer. The anticancer effect of Dox-DNA-AuNP was evaluated in vitro using the EZ-Cytox cell viability assay on three human ovarian cancer cell lines, SK-OV-3, HEY A8, and A2780. Dox-DNA-AuNP exhibited outstanding activity with good IC50 values of 4.8, 7.4, and 7.6 nM for SK-OV-3, HEY A8, and A2780, respectively. In vivo evaluation further demonstrated the superior anticancer effects of Dox-DNA-AuNP by inhibiting tumor growth compared to free Dox in an established SK-OV-3 xenograft mice model. Dox-DNA-AuNP showed about a 2.5 times higher tumor growth inhibition rate than free Dox. Furthermore, the immunohistochemical analysis of Ki67 antigen expression showed the lowest number of proliferative cells in the ovarian tumor tissue treated with Dox-DNA-AuNP. These results suggest Dox-DNA-AuNP might be a potential effective agent in ovarian cancer chemotherapy

Persistent impacts of smoking on resting-state EEG in male chronic smokers and past-smokers with 20 years of abstinence

This work was supported in part by UNIST internal funding (1.210046.01) and the National Research Foundation of Korea (NRF-2018R1D1A1B07043582 to Chung). K.B. was partly supported by the National Research Foundation of Korea (NRF-2021R1F1A1063968), Institute of Information & Communications Technology Planning & Evaluation (Grant No. 2020-0-01450, Artificial Intelligence Convergence Research Center [Pusan National University]), and Ministry of Education of South Korea (the BK21 Four program, Korean Southeast Center for the 4th Industrial Revolution Leader Education)

Identification of <i>Polygonum orientale</i> constituents using high-performance liquid chromatography high-resolution tandem mass spectrometry

<p>Our primary focus in this research was to identify and characterize its bioactive compounds for potential therapeutic use. Twenty-seven metabolites of <i>Polygonum orientale</i> were identified using LC-QTOF tandem mass spectrometry. Interestingly, <i>P. orientale</i> extracts included several highly oxygenated flavonoids were isolated from <i>P. orientale</i> by column chromatography. ¹³C NMR data of highly oxygenated flavonoids (<b>1–7</b>) are reported here for the first time. In addition, nitric oxide, 1,1-diphenyl-2-picrylhydrazyl, and water-soluble tetrazolium salt assays were carried out on the isolated compounds to investigate their anti-inflammatory, anti-oxidant, and neuroprotective activities, respectively. Compounds <b>1</b>, <b>2</b>, <b>3</b>, <b>5</b>, <b>7</b>, and <b>8</b> significantly attenuated lipopolysaccharide-stimulated NO production in BV2 cells without affecting cell viability. Compounds <b>9–12</b> exhibited significant antioxidant activity, while compounds <b>8</b>, <b>9,</b> and <b>12</b> exhibited protective effects against glutamate-induced neurotoxicity in HT22 cells. Our results indicate that <i>P. orientale</i> is a promising source of natural agents for the potential treatment of inflammation and neurodegenerative diseases.</p> <p>Chemical profile of <i>Polygonum orientale</i>.</p