Space Suits and Crew Survival Systems Branch Education and Public Outreach Support of NASA's Strategic Goals in Fiscal Year 2012

As NASA plans to send people beyond low Earth orbit, it is important to educate and inspire the next generation of astronauts, engineers, scientist, and general public. This is so important to NASA future that it is one of the agencies strategic goals. The Space Suits and Crew Survival Systems Branch at Johnson Space Center (JSC) is actively involved in helping to achieve this goal by sharing our hardware and technical experts with students, educators, and the general public and educating them about the challenges of human space flight, with Education and Public Outreach (EPO). This paper summarizes the Space Suit and Crew Survival Systems Branch EPO efforts throughout fiscal year 2012

Requirements and Sizing Investigation for Constellation Space Suit Portable Life Support System Trace Contaminant Control

The Trace Contaminant Control System (TCCS), located within the ventilation loop of the Constellation Space Suit Portable Life Support System (PLSS), is responsible for removing hazardous trace contaminants from the space suit ventilation flow. This paper summarizes the results of a trade study that evaluated if trace contaminant control could be accomplished without a TCCS, relying on suit leakage, ullage loss from the carbon dioxide and humidity control system, and other factors. Trace contaminant generation rates were revisited to verify that values reflect the latest designs for Constellation Space Suit System (CSSS) pressure garment materials and PLSS hardware. Additionally, TCCS sizing calculations were performed and a literature survey was conducted to review the latest developments in trace contaminant technologies

Crew and Thermal Systems Strategic Communications Initiatives in Support of NASA's Strategic Goals

NASA has defined strategic goals to invest in next-generation technologies and innovations, inspire students to become the future leaders of space exploration, and expand partnerships with industry and academia around the world. The Crew and Thermal Systems Division (CTSD) at the NASA Johnson Space Center actively supports these NASA initiatives. In July 2011, CTSD created a strategic communications team to communicate CTSD capabilities, technologies, and personnel to external technical audiences for business development and collaborative initiatives, and to students, educators, and the general public for education and public outreach efforts. This paper summarizes the CTSD Strategic Communications efforts and metrics through the first half of fiscal year 2012 with projections for end of fiscal year data

Performance and Life Tests of a Regenerative Blower for EVA Suit Ventilation

Ventilation fans for future space suits must meet demanding performance specifications, satisfy stringent safety requirements for operation in an oxygen atmosphere, and be able to increase output to operate in buddy mode. A regenerative blower is an attractive choice due to its ability to meet these requirements at low operating speed. This paper describes progress in the development and testing of a regenerative blower designed to meet requirements for ventilation subsystems in a future space suit Portable Life Support Systems (PLSS). The blower assembly includes a custom-designed motor that has significantly improved in efficiency during this development effort. The blower was tested at both nominal and buddy mode operating points and head/flow performance and power consumption were measured. The blower was operated for over 1000 hours to demonstrate safe operation in an oxygen test loop at prototypical pressures. In addition, the blower demonstrated operation with the introduction of simulated lunar dust

Extravehicular Activity Systems Education and Public Outreach in Support of NASA's STEM Initiatives in Fiscal Year 2011

NASA's goals to send humans beyond low Earth orbit will involve the need for a strong engineering workforce. Research indicates that student interest in science, technology, engineering, and math (STEM) areas is on the decline. According to the Department of Education, the United States President has mandated that 100,000 educators be trained in STEM over the next decade to reduce this trend. NASA has aligned its Education and Public Outreach (EPO) initiatives to include emphasis in promoting STEM. The Extravehicular Activity (EVA) Systems Project Office at the NASA Johnson Space Center actively supports this NASA initiative by providing subject matter experts and hands-on, interactive presentations to educate students, educators, and the general public about the design challenges encountered as NASA develops EVA hardware for exploration missions. This paper summarizes the EVA Systems EPO efforts and metrics from fiscal year 2011

Redesign of the Extravehicular Mobility Unit Airlock Cooling Loop Recovery Assembly

During EVA (Extravehicular Activity) 23 aboard the ISS (International Space Station) on 07/16/2013 an episode of water in the EMU (Extravehicular Mobility Unit) helmet occurred, necessitating a termination of the EVA (Extravehicular Activity) shortly after it began. The root cause of the failure was determined to be ground-processing short-comings of the ALCLR (Airlock Cooling Loop Recovery) Ion Beds which led to various levels of contaminants being introduced into the Ion Beds before they left the ground. The Ion Beds were thereafter used to scrub the failed EMU cooling water loop on-orbit during routine scrubbing operations. The root cause investigation identified several areas for improvement of the ALCLR Assembly which have since been initiated. Enhanced washing techniques for the ALCLR Ion Bed have been developed and implemented. On-orbit cooling water conductivity and pH analysis capability to allow the astronauts to monitor proper operation of the ALCLR Ion Bed during scrubbing operation is being investigation. A simplified means to acquire on-orbit EMU cooling water samples have been designed. Finally, an inherently cleaner organic adsorbent to replace the current lignite-based activated carbon, and a non-separable replacement for the separable mixed ion exchange resin are undergoing evaluation. These efforts are undertaken to enhance the performance and reduce the risk associated with operations to ensure the long-term health of the EMU cooling water circuit

Advanced Prototype Fan Operating Experience, Post Test Evaluation, and Refurbishment for PLSS 2.0 Test Use

NASA s plans for Extravehicular Activity (EVA) portable life support systems for future exploration missions result in different design requirements than those which led to the combined fan / pump / separator in the current ISS Extravehicular Mobility Unit (EMU). To meet these new requirements, NASA contracted with Hamilton Sundstrand to provide two new prototype fans designed to meet anticipated future system requirements. Based on design trade studies, a high speed fan with mechanical bearing support of the rotating elements and a novel non-metallic barrier canned motor design was developed and implemented in the deliverable prototypes. The prototypes, which used two different bearing lubricants, have been extensively tested in both stand-alone and integrated system tests in NASA laboratories and proven to meet the anticipated performance requirements. Subsequently, they have been subjected to post test inspection and analysis in Hamilton Sundstrand laboratories to assess the effects of integrated operation and resultant exposure to vent loop contaminants. Results have confirmed expectations that one of the lubricants would be superior in this application and the prototype fans have been reassembled with new bearings with the superior lubricant. They have now been returned to the Johnson Space Center for further testing and maturation as part of NASA s PLSS 2.0 integrated test effort. This paper will discuss the test history of these units, resulting test data, the results of post test evaluation, and plans for further testing in the near future

Performance and Life Tests of a Regenerative Blower for EVA Suit Ventilation

Ventilation fans for future space suits must meet demanding performance specifications, satisfy stringent safety requirements for operation in an oxygen atmosphere, and be able to increase output to operate in buddy mode. A regenerative blower is an attractive choice due to its ability to meet these requirements at low operating speed. This paper describes progress in the development and testing of a regenerative blower designed to meet requirements for ventilation subsystems in future space suits. The blower includes a custom-designed motor that has significantly improved its efficiency. We have measured the blower s head/flow performance and power consumption under conditions that simulate both the normal and buddy mode operating points. We have operated the blower for TBD hours and demonstrated safe operation in an oxygen test loop at prototypical pressures. We also demonstrated operation with simulated lunar dust

Requirements and Sizing Investigation for the Constellation Space Suit Portable Life Support System Trace Contaminant Control

This presentation summarized the results of a trade study that evaluated whether trace contaminant control within the Constellation Spacesuit PLSS could be achieved without a Trace Contaminant Control System (TCCS) by relying on suit leakage, ullage loss from the carbon dioxide and humidity control system, and other factors. Mallory Jennings and Dr. Glenn Waguespack studied trace contaminant generation rates to verify that values reflected the latest designs for Constellation spacesuit system pressure garment materials and PLSS hardware. They also calculated TCCS sizing and conducted a literature survey to review the latest developments in trace contaminant technologies

Gene expression analysis of cell death induction by Taurolidine in different malignant cell lines

<p>Abstract</p> <p>Background</p> <p>The anti-infective agent Taurolidine (TRD) has been shown to have cell death inducing properties, but the mechanism of its action is largely unknown. The aim of this study was to identify potential common target genes modulated at the transcriptional level following TRD treatment in tumour cell lines originating from different cancer types.</p> <p>Methods</p> <p>Five different malignant cell lines (HT29, Chang Liver, HT1080, AsPC-1 and BxPC-3) were incubated with TRD (100 μM, 250 μM and 1000 μM). Proliferation after 8 h and cell viability after 24 h were analyzed by BrdU assay and FACS analysis, respectively. Gene expression analyses were carried out using the <it>Agilent </it>-microarray platform to indentify genes which displayed conjoint regulation following the addition of TRD in all cell lines. Candidate genes were subjected to <it>Ingenuity Pathways Analysis </it>and selected genes were validated by qRT-PCR and Western Blot.</p> <p>Results</p> <p>TRD 250 μM caused a significant inhibition of proliferation as well as apoptotic cell death in all cell lines. Among cell death associated genes with the strongest regulation in gene expression, we identified pro-apoptotic transcription factors (EGR1, ATF3) as well as genes involved in the ER stress response (PPP1R15A), in ubiquitination (TRAF6) and mitochondrial apoptotic pathways (PMAIP1).</p> <p>Conclusions</p> <p>This is the first conjoint analysis of potential target genes of TRD which was performed simultaneously in different malignant cell lines. The results indicate that TRD might be involved in different signal transduction pathways leading to apoptosis.</p