Fin-mutant Female Zebrafish (Danio rerio) Exhibit Differences in Association Preferences for Male Fin Length

Females often choose to associate with males that have exaggerated traits. In fishes, this may reflect an overall preference for larger size in a potential mate. Female zebrafish (Danio rerio) prefer males with larger bodies but not longer fins. The availability of mutant and transgenic strains of zebrafish make this a unique model system in which to study the role of phenotypic variation in social and sexual behavior. We used mutant strains of zebrafish with truncated (short fin) and exaggerated (long fin) fins to further examine female preferences for fin length in dichotomous association tests. Wild type females showed no preferences between wild type males and short fin mutant males or between wild type males and long fin mutant males. short fin females also showed no preference for short fin males or wild type males while long fin females preferred to associate with long fin males over wild type males. These results suggest that the single gene long fin mutation that results in altered fin morphological may also be involved in a related female association preference

Serial monogamy benefits both sexes in the biparental convict cichlid

Monogamy can be either long-term or serial, with new pairs formed with each breeding bout. Costs and benefits are associated with each strategy. Because biparental convict cichlids (Amatitlania nigrofasciata) typically switch mates, exhibiting serial monogamy, we tested for the costs associated with forcing individuals to remain with the same mate. Convict cichlids were observed over two successive breeding bouts, either with the same or a new, equally experienced, mate. Parental behavior did not differ between breeding bouts, nor did brood size. Surprisingly, fish that remained with their original partner for a second bout took significantly longer to produce a brood compared to fish that paired with new partners. New partners were also more likely to successfully produce a second brood than re-mated partners. This is in contrast to the majority of bird studies that show many benefits to staying with the same partner for multiple broods. In convict cichlids, there seems to be no benefit associated with remaining with the same partner and switching mates reduces duration between broods for both males and females, potentially increasing overall reproductive success

Conservation and conflict between endangered desert fishes

Conservation of naturally sympatric endangered species requires unique considerations. While impacts of invasive species garner much attention, interactions between endangered species must also be managed. The endangered Leon Springs pupfish, Cyprinodon bovinus, has suffered a population decline due to decreasing natural habitat. As breeding habitat is lost, C. bovinus is also adversely affected by the sympatric, endangered Pecos gambusia, Gambusia nobilis. Here, we document interactions between these species, finding significantly more G. nobilis accumulated at pupfish spawning events than randomly distributed on breeding grounds in the absence of spawning. As a known egg predator, our results suggest that G. nobilis presence at spawnings may further decrease pupfish numbers while also altering the evolutionary dynamics of C. bovinus breeding tactics. Habitat restoration may decrease Gambusia concentrations or influence C. bovinus breeding behaviour and increase the number of territorial males resulting in viable population sizes for both critically endangered fishes

Male convict cichlid 11-ketotestosterone levels throughout the reproductive cycle: an exploratory profile study in laboratory and field populations

The convict cichlid (Amatitlania nigrofasciata) has been extensively examined in relation to many behavioral topics, such as courtship, pair-bonding, bi-parental care, and territoriality. Recently, this model species has been utilized in studies on genetics, endocrinology, and neuroanatomy, with an ultimate goal of connecting behavior with its underlying mechanisms. The goal of this study was two-fold: (1) profile the circulating levels of plasma 11KT in the male convict cichlid at multiple points during the reproductive cycle and (2) generally compare the hormonal profiles of the widely used laboratory populations and those of a free-living population in the streams of Costa Rica. The results of the field experiment showed that male convict cichlids had higher levels of circulating 11KT during courtship and lower during the parental care and non-breeding phases. The profile of the laboratory population was similar to the profile of the free-living individuals, with significantly higher levels of 11KT occurring during courtship than during parental care, though the level of 11KT during non-breeding phase was elevated in the laboratory. The high levels of 11KT during courtship and low levels of 11KT during parental care found in both the field and the laboratory is similar to what has been reported in other species of teleosts, and may suggest an important function of 11KT in the expression of courtship behavior and the subsequent onset of parental behaviors in this model species

The Influence of Sex and Phenotype on Shoaling Decisions in Zebrafish

Fish typically choose shoalmates with similar phenotypic characteristics to themselves, thus creating shoals for which predators have difficulty identifying and attacking one specific individual. And while shoaling should provide similar anti-predator benefits to both males and females, the two sexes do not always make the same shoaling decisions. Here we explore the effect of phenotype on sex specificshoaling in three varieties of zebrafish (Danio rerio) and the closely related pearl danio(Danio albolineatus). We hypothesized that males and females of each type of zebrafish (wildtype, golden mutants and leopard mutants), as well as male and female pearl danios, would choose to shoal rather than be alone and, when given a choice of shoalmates, would shoal with fish of their own phenotype rather than dissimilar fish. As expected, our results show that most fish preferred to shoal rather than be alone. However, while both sexes of wildtype zebrafish responded identically to shoaling decisions, male and female mutant zebrafish and pearl danio fish differed in their response to such choices. When given a choice of shoalmates, wildtype zebrafish of both sexes showed no discrimination between different D. rerio strains, although they did choose to shoal with wild type conspecifics rather than pearl danios. The shoalmate preferences of the mutant zebrafish revealed that males showed no discrimination between shoals of their own variety and wildtype shoals, while mutant females preferred shoals of their own strain. Similarly, male pearl danios showed no discrimination between shoals of their own species and shoals of wildtype zebrafish, while pearl danio females preferred their own species. These results demonstrate the complex influence of sex and phenotype on shoaling behavior

Assessment and decision-making in a Caribbean damselfish: nest-site quality influences prioritization of courtship and brood defence

In systems where territory quality varies, animals are expected to exhibit plasticity in behaviour in order to maximize fitness relative to their present territory quality. This requires assessment of territory quality followed by decision-making in relation to the priority of activities necessary for survival and reproduction. We examined how differences in territory quality of beaugregory damselfish (Stegastes leucostictus) influence the prioritization of courtship and egg defence by comparing behavioural responses of males defending artificial sites (high quality) with males defending natural sites (low quality) when presented with an egg predator, a conspecific female, and a simultaneous choice between both. A significant three-way interaction of territory quality, presentation type and stimulus was observed for time near stimuli. In paired presentations, males defending low-quality territories spent more time near a female and less near an egg predator; while males on high-quality territories spent more time near a predator than a female. Additionally, comparing single and paired presentations reveals that behaviours towards egg predators remain constant while behaviours towards females decrease with paired stimuli. These data suggest that territory quality and ecological context impact decision-making and the relative values of potential reproduction and/or past reproductive effort

Management of Interactions Between Endangered Species Using Habitat Restoration

Commonly used conservation strategies may be insufficient when deleterious interactions between cohabiting endemic species occur. The decline in the population size of the endangered Leon Springs pup- fish, Cyprinodon bovinus, in Diamond Y Spring, Texas has been partially attributed to egg-predation by the endangered Pecos gambusia, Gambusia nobilis. This interaction is related to changes in habitat availability; therefore, we aim to manage the conflict via restoration of the breeding habitat. We hypothesized that altering the habitat to expand shallow breeding areas would result in a decrease in the number of gambusia preying on the eggs of spawning pupfish pairs and an increase in the number of males defending territories. In 2 years following the habitat modification, we observed resurgence in the pupfish breeding population and a decrease in egg-predation pressure around spawning pupfish pairs. Additionally, after altering the habitat, gambusia were more dispersed throughout the habitat, as the low numbers of gambusia in a territory were stable regardless of spawning or aggressive behaviors by pupfish. To our knowledge, this is the first empirical example of effective management of deleterious interactions between two endangered species by means of habitat restoration

Caribbean damselfish with varying territory quality: correlated behaviors but not a syndrome

The behavioral syndrome hypothesis suggests that individual animals within a population behave differently due to specific behavioral types, and these should be consistent across behaviors or in different contexts. In contrast, for animals that live within an environment in which territory quality can change over time, natural selection should have favored behavioral flexibility and modulation of the cost of defense in relation to territory quality. This would require assessment of the territory followed by displays of appropriate types and intensities of behavior. We examined the territorial behavior of male beaugregory damselfish (Stegastes leucostictus) by enhancing territory quality using artificial breeding sites and comparing their behavior to males on lower quality natural sites. When male fish were defending high-quality artificial territories, they had higher levels of aggression toward male conspecifics and courtship toward females than when on low-quality natural territories. We also found that aggression and courtship behaviors were correlated on natural sites but not on artificial sites. Behaviors were not correlated within individuals when males switched from natural to artificial territories or from artificial to natural territories. These results indicate that males assess their current territories and adjust behaviors accordingly and that courtship and aggressive behaviors are not linked within a permanent behavioral syndrome. Copyright 2009, Oxford University Press.