The Grizzly, October 4, 2018

We Ought to Listen : Professors Gather with Students to Watch Kavanaugh-Ford Hearings • Where\u27s the Money?: Students Reflect on Their Unpaid and Paid Summer Internship Experiences • How Students Feel About Tuition Increases (Badly): Paying for School can be Stressful • Where Does Your Tuition Go? • Students Investing Real Money • Opinions: A Look Back at the 2008 Financial Crisis; Real Cost of Applying to Medical Schools; Division I Athletes Should Not be Paid • Hunter Tabbed for Associate ADhttps://digitalcommons.ursinus.edu/grizzlynews/1603/thumbnail.jp

The Grizzly, October 11, 2018

Student Athletes Distressed by Possible NCAA Violations • Clinica de Migrantes Screens on Campus • Externship Deadline is Rapidly Approaching • Ursinus\u27 Website Receives a New Makeover • Sigma Rho Lambda: Past, Present, Future • Finding Truth: 12 Angry Jurors • Opinions: Juuls Shouldn\u27t be Marketed to Teenagers; Family Separations a Lingering Problem • Ursinus Mourns Loss of Coach Racich • Men\u27s Golf Flourishes in its Opening Matcheshttps://digitalcommons.ursinus.edu/grizzlynews/1604/thumbnail.jp

The Grizzly, October 12, 2017

USAS Fights to In-source Ursinus Cleaning Staff • Ursinus Releases 2017 Annual Security and Fire Safety Report • Dr. April Carpenter and her Team of Students Challenge the Rest, Ice, Compression, Elevate Approach to Recovery • Several Uninvited Visitors Reported in Reimert Courtyard • Sustainability Week Brought Fall Fun to Ursinus • Digital Aid for Humanities • Opinions: Puerto Rico Deserves Federal Assistance after Maria; Meal Plan Options Should Include Opt-out Opportunity • Mid-season Check-in with Ursinus College Field Hockey • Bears Upset Blue Jays, Remain Atop CChttps://digitalcommons.ursinus.edu/grizzlynews/1628/thumbnail.jp

The Grizzly, September 13, 2018

Reim Time? Not Until After 8 P.M. • Welcoming the New Chaplain and Director of Religious and Spiritual Life • Pre-plated Portions Prove Perplexing, Promising? • Ursinus is Crafting a Values Statement • IDC, But They Do: Professors Weigh In On the IDC • Return of the Ursinusaur • Opinion: Betsy DeVos\u27 Proposed Changes Would be Harmful; Elon Musk is Not That Great • Massive First-Year Football Class Adjusts to Life at Ursinus • Field Hockey Aims to Regain Centennial Conference Supremacyhttps://digitalcommons.ursinus.edu/grizzlynews/1936/thumbnail.jp

The Grizzly, November 15, 2018

Mythbuster: Student Writer Takes on Medusa and Marginalization • Voter Registration Status Causes Issues for Student Voters on Election Day • Members of the Ursinus Community Gather to Remember Victims of the Tree of Life Shooting • What do UC Political Clubs Think About the Recent Elections? • Why Myrin is Full of Government Documents • Opinions: Choosing Classes Needs to be Less Confusing; We Need to Better Recognize World War I Veterans • Fall Sports Seniors Complete Final Seasons • McDaid Makes Ursinus Cross Country History • Q&A with Mike Moronesehttps://digitalcommons.ursinus.edu/grizzlynews/1608/thumbnail.jp

The Grizzly, November 29, 2018

Ursinus Alum Receives Prestigious Fellowship • Student Discusses Journey with Brain Surgery • Members of Ursinus Community Gathered for 3rd Annual Celebration of Lights • Ursinus and the Armed Forces • Farm Fellowship and the Ursinus Campus Farm • Opinions: Venom: On Capes, Camp, and the Case for Cheese; Abolishing ICE Isn\u27t Radical, it\u27s Obvious • Wrestling Senior Reflects on Lessons Learned from Coach Racich • Football Caps Season with Four Straight Wins, Takes Centennial / MAC Bowl Titlehttps://digitalcommons.ursinus.edu/grizzlynews/1609/thumbnail.jp

Proteomics-Based Approach for Detailing the Allergenic Profile of Cannabis Chemotypes

Allergic sensitization to cannabis is an emerging public health concern and is difficult to clinically establish owing to lack of standardized diagnostic approaches. Attempts to develop diagnostic tools were largely hampered by the Schedule I restrictions on cannabis, which limited accessibility for research. Recently, however, hemp was removed from the classified list, and increased accessibility to hemp allows for the evaluation of its practical clinical value for allergy diagnosis. We hypothesized that the proteomic profile is preserved across different cannabis chemotypes and that hemp would be an ideal source of plant material for clinical testing. Using a proteomics-based approach, we examined whether distinct varieties of cannabis plant contain relevant allergens of cannabis. Cannabis extracts were generated from high tetrahydrocannabinol variety (Mx), high cannabidiol variety (V1-19) and mixed profile variety (B5) using a Plant Total Protein Extraction Kit. Hemp extracts were generated using other standardized methods. Protein samples were subjected to nanoscale tandem mass spectrometry. Acquired peptides sequences were examined against the Cannabis sativa database to establish protein identity. Non-specific lipid transfer protein (Can s 3) level was measured using a recently developed ELISA 2.0 assay. Proteomic analysis identified 49 distinct potential allergens in protein extracts from all chemotypes. Most importantly, clinically relevant and validated allergens, such as profilin (Can s 2), Can s 3 and Bet v 1-domain-containing protein 10 (Can s 5), were identified in all chemotypes at label-free quantification (LFP) intensities \u3e 106. However, the oxygen evolving enhancer protein 2 (Can s 4) was not detected in any of the protein samples. Similarly, Can s 2, Can s 3 and Can s 5 peptides were also detected in hemp protein extracts. The validation of these findings using the ELISA 2.0 assay indicated that hemp extract contains 30-37 ng of Can s 3 allergen per µg of total protein. Our proteomic studies indicate that relevant cannabis allergens are consistently expressed across distinct cannabis chemotypes. Further, hemp may serve as an ideal practical substitute for clinical testing, since it expresses most allergens relevant to cannabis sensitization, including the validated major allergen Can s 3

Proteomics-Based Approach for Detailing the Allergenic Profile of Cannabis Chemotypes

Allergic sensitization to cannabis is an emerging public health concern and is difficult to clinically establish owing to lack of standardized diagnostic approaches. Attempts to develop diagnostic tools were largely hampered by the Schedule I restrictions on cannabis, which limited accessibility for research. Recently, however, hemp was removed from the classified list, and increased accessibility to hemp allows for the evaluation of its practical clinical value for allergy diagnosis. We hypothesized that the proteomic profile is preserved across different cannabis chemotypes and that hemp would be an ideal source of plant material for clinical testing. Using a proteomics-based approach, we examined whether distinct varieties of cannabis plant contain relevant allergens of cannabis. Cannabis extracts were generated from high tetrahydrocannabinol variety (Mx), high cannabidiol variety (V1-19) and mixed profile variety (B5) using a Plant Total Protein Extraction Kit. Hemp extracts were generated using other standardized methods. Protein samples were subjected to nanoscale tandem mass spectrometry. Acquired peptides sequences were examined against the Cannabis sativa database to establish protein identity. Non-specific lipid transfer protein (Can s 3) level was measured using a recently developed ELISA 2.0 assay. Proteomic analysis identified 49 distinct potential allergens in protein extracts from all chemotypes. Most importantly, clinically relevant and validated allergens, such as profilin (Can s 2), Can s 3 and Bet v 1-domain-containing protein 10 (Can s 5), were identified in all chemotypes at label-free quantification (LFP) intensities > 106. However, the oxygen evolving enhancer protein 2 (Can s 4) was not detected in any of the protein samples. Similarly, Can s 2, Can s 3 and Can s 5 peptides were also detected in hemp protein extracts. The validation of these findings using the ELISA 2.0 assay indicated that hemp extract contains 30–37 ng of Can s 3 allergen per µg of total protein. Our proteomic studies indicate that relevant cannabis allergens are consistently expressed across distinct cannabis chemotypes. Further, hemp may serve as an ideal practical substitute for clinical testing, since it expresses most allergens relevant to cannabis sensitization, including the validated major allergen Can s 3