Isospin-Breaking quark condensates in Chiral Perturbation Theory

We analyze the isospin-breaking corrections to quark condensates within one-loop SU(2) and SU(3) Chiral Perturbation Theory including $m_u\neq m_d$ as well as electromagnetic (EM) contributions. The explicit expressions are given and several phenomenological aspects are studied. We analyze the sensitivity of recent condensate determinations to the EM low-energy constants (LEC). If the explicit chiral symmetry breaking induced by EM terms generates a ferromagnetic-like response of the vacuum, as in the case of quark masses, the increasing of the order parameter implies constraints for the EM LEC, which we check with different estimates in the literature. In addition, we extend the sum rule relating quark condensate ratios in SU(3) to include EM corrections, which are of the same order as the $m_u\neq m_d$ ones, and we use that sum rule to estimate the vacuum asymmetry within ChPT. We also discuss the matching conditions between the SU(2) and SU(3) LEC involved in the condensates, when both isospin-breaking sources are taken into account.Comment: 16 pages, 1 figure, final version accepted for publication in Journal of Physics

SUICIDE ATTEMPTS AMONG GREEK AND DANISH WOMEN AND THE QUALITY OF THEIR RELATIONSHIPS WITH HUSBANDS OR BOYFRIENDS

Greek and Danish women 15-45 years old who attempted suicide were interviewed with an extensive semistructured schedule, in Athens and Copenhagen, after admission to the intensive care unit of 2 general hospitals and one psychiatric reception ward. This article presents 2 groups from each country, a group of married women and a group with steady relationships (n = 56), focusing on the quality of their relationships and’ their significance for the women’s suicide attempts. Most of the women in both countries had very poor relationships, as concerns communication, intimacy, concern, recreation, sharing of everyday activities and social and private life. A high degree of psychological and physical violence was found in all 4 groups. The burdensome and humiliating relationship created shame in the women. After unsuccessful attempts to change the relationship and inability to leave it because of financial and emotional dependence, the women attempted suicide to escape from the situation

The spleen of patients with myelofibrosis harbors defective mesenchymal stromal cells

Splenic hematopoiesis is a major feature in the course of myelofibrosis (MF). In fact, the spleen of patients with MF contains malignant hematopoietic stem cells retaining a complete differentiation program, suggesting both a pivotal role of the spleen in maintaining the disease and a tight regulation of hematopoiesis by the splenic microenvironment, in particular by mesenchymal stromal cells (MSCs). Little is known about splenic MSCs (Sp-MSCs), both in normal and in pathological context. In this work, we have in vitro expanded and characterized Sp-MSCs from 25 patients with MF and 13 healthy subjects (HS). They shared similar phenotype, growth kinetics, and differentiation capacity. However, MF Sp-MSCs expressed significant lower levels of nestin, and favored megakaryocyte (Mk) differentiation in vitro at a larger extent than their normal counterpart. Moreover, they showed a significant upregulation of matrix metalloprotease 2 (MMP2) and fibronectin 1 (FN1) genes both at mRNA expression and at protein level, and, finally, developed genetic abnormalities which were never detected in HS-derived Sp-MSCs. Our data point toward the existence of a defective splenic niche in patients with MF that could be responsible of some pathological features of the disease, including the increased trafficking of CD34+ cells and the expansion of the megakaryocytic lineage

Tie2 expressing monocytes in the spleen of patients with primary myelofibrosis

Primary myelofibrosis (PMF) is a Philadelphia-negative (Ph-) myeloproliferative disorder, showing abnormal CD34 + progenitor cell trafficking, splenomegaly, marrow fibrosis leading to extensive extramedullary haematopoiesis, and abnormal neoangiogenesis in either the bone marrow or the spleen. Monocytes expressing the angiopoietin-2 receptor (Tie2) have been shown to support abnormal angiogenic processes in solid tumors through a paracrine action that takes place in proximity to the vessels. In this study we investigated the frequency of Tie2 expressing monocytes in the spleen tissue samples of patients with PMF, and healthy subjects (CTRLs), and evaluated their possible role in favouring spleen angiogenesis. We show by confocal microscopy that in the spleen tissue of patients with PMF, but not of CTRLs, the most of the CD14 + cells are Tie2 + and are close to vessels; by flow cytometry, we found that Tie2 expressing monocytes were Tie2 + CD14 low CD16 bright CDL62 - CCR2 - (TEMs) and their frequency was higher (p = 0.008) in spleen tissue-derived mononuclear cells (MNCs) of patients with PMF than in spleen tissue-derived MNCs from CTRLs undergoing splenectomy for abdominal trauma. By in vitro angiogenesis assay we evidenced that conditioned medium of immunomagnetically selected spleen tissue derived CD14 + cells of patients with PMF induced a denser tube like net than that of CTRLs; in addition, CD14 + Tie2 + cells sorted from spleen tissue derived single cell suspension of patients with PMF show a higher expression of genes involved in angiogenesis than that found in CTRLs. Our results document the enrichment of Tie2 + monocytes expressing angiogenic genes in the spleen of patients with PMF, suggesting a role for these cells in starting/maintaining the pathological angiogenesis in this organ