Maize core collection for increased grain quality

Formation of the maize (Zea mays L) core collection for increased macronutrient content is presented. Among 3,443 populations from Maize Research Institute (MRI), Zemun Polje (ZP), a total of 352 accessions (around 10.2%) were chosen for the core collection. The criteria were increased protein, oil and/or starch content in the kernel of the accessions, as well as good combining ability (CA) that was tested with two Lancaster and one each BSSS and Iodent testers. Average values for protein, oil and starch content for the whole collection were 11.5%, 4.2% and 68.9%, respectively. On the other hand, averages for the core collection were 13.6% for proteins, 7.7% for oil and 74.1% for starch, and they were significantly higher than for the whole collection (

Uticaj spoljašnje sredine na segregacioni odnos kvalitativnih svojstava kukuruza

The significant influence of environment was found on the segregation ratio in a dyhibrid inheritance in maize. Two possible causes are proposed for this segregation distortion: 1) environmental influence (selection) prior forming the gametes or/and 2) different preferential pairing in different environments. Further studies, however, on other self or cross-pollinated plant species, and with different traits are needed to better understand this phenomena.Značajan uticaj spoljašnje sredine na segregacioni odnos je utvrđen kod dihibridnog nasleđivanja kod kukuruza. Dva moguća uzorka segregacione distorzije su: 1) uticaj spoljašnje sredine (selekcija) pre formiranja gameta ili/i 2) različita preferencijalna ukrštanja u različitim sredinama. Dalja istraživanja, na drugim samooplodnim ili stranooplodnim biljnim vrstama i sa različitim svojstvima su potrebna da bi se ovaj fenomen bolje razumeo

The effects of cytoplasmic male sterility and xenia on the chemical composition of maize grain

Sterile hybrids often outyield their fertile counterparts, especially if pollinated by a genetically unrelated pollinator. The combined effect of cms and xenia is referred to as the Plus-hybrid effect. The objective of this study was to determine the individual, as well as, combining effect of cms and xenia on the maize grain chemical composition. The percent of oil, protein and starch in the grain was also observed. Two sterile hybrids, their fertile counterparts and five fertile pollinator-hybrids were selected for the studies. The three-replicate trial set up according to the split-plot experimental design was performed at Zemun Polje in 2008. The obtained results show that the effects of cms on the oil percent was not significant in the studied hybrid ZP 341, while it increased at the significance level of P = 0.1 in the second observed hybrid ZP 360. The effect of this factor on the protein and starch percent was also significant (P = 0.01) in some hybrid combinations. Xenia effects on all three chemical parameters were significant (P = 0.01) in some hybrid combinations. The gained results indicate that the identification of a good combination of two hybrids, in which one would be a sterile female component, and the other a pollinator, would end up not only in the increased yield, but also in the improved maize grain quality

Uticaj citoplazmatičke muške sterilnosti i ksenija na komponente prinosa kukuruza

The combined effect of cms and xenia is referred to as the plus-hybrid effect and can be used in the maize production in order to increase maize grain yield and quality by growing the sterile version of one hybrid and the fertile version of another hybrid. Two sterile hybrids, their fertile counterparts and five fertile hybrids as pollinators were selected. The following traits were studied: ear length, kernel row number, number of kernels per row, kernel length and 1000-kernel weight. All plus-hybrid combinations of the first hybrid had a longer ear than their fertile counterpart, while this value was reduced in the second hybrid in three out of four combinations. The greatest increase in the ear length amounted to 3.04% (P=0.05). Although all plus-hybrid combinations of the first hybrid had a smaller kernel row number, the differences were not significant. All plus-hybrid combinations of the second hybrid had higher value of this observed trait, while the value of the best combination was higher by 4.2% than the value of its fertile counterpart (P=0.1). As far as the number of kernels per row is concerned, there were no significant differences between the plus-hybrid combinations and their fertile counterparts in any of observed hybrids. The plus-hybrid effect on the kernel length was positive in both hybrids. The best plus-hybrid combination of the first, i.e. the second hybrid had value of this trait higher by 6.06% (P=0.1), i.e. 4.07% (P=0.05), respectively. Two plus-hybrid combinations of the first hybrid had a higher 1000-kernel weight than their fertile counterparts. These differences were not significant. The value of the trait in the worst plus-hybrid combination of the second hybrid was lower by even 12.18% than the value of the fertile counterpart (P=0.01).Kombinovani efekat cms-a i ksenija naziva se Plus-hibrid kukuruza. Odabrali smo dva sterilna hibrida kukuruza, njihove fertilne analoge i pet fertilnih hibrida oprašivača. Ispitivana je dužinu klipa, broj redova zrna, broj zrna u redu, dužinu zrna i masa 1000 zrna. Dok su sve Plus-hibrid kombinacije prvog hibrida imale duži klip u odnosu na fertilnu verziju, kod drugog hibrida je došlo do smanjenja ove vrednosti kod tri od četiri kombinacije. Najviše povećanje dužine klipa iznosilo je 3,04% (P=0,05). Iako su sve Plus-hibrid kombinacije prvog hibrida imale manju vrednost broja redova zrna, ove razlike nisu bile statistički značajne. Sve Plus-hibrid kombinacije drugog hibrida su pokazale povećanje vrednosti ovog ispitivanog svojstva, a najbolja je imala za 4,2% višu vrednost od svog fertilnog analoga (P=0,1). Za svojstvo broj zrna u redu nije bilo značajne razlike između Plus-hibrid kombinacija i fertilnih analoga ni kod jednog od ispitivanih hibrida. Plus-hibrid efekat na dužinu zrna, kod oba hibrida, bio je pozitivan. Najbolja Plus-hibrid kombinacija prvog hibrida imala je za 6,06% (P=0,1), a najbolja kombinacija drugog hibrida za 4,07% (P=0,05) višu vrednost ovog ispitivanog svojstva. Dve Plus-hibrid kombinacije prvog hibrida imale su povećanu masu 1000 zrna u odnosu na svog fertilnog analoga, ali ne značajno. Kod drugog ispitivanog hibrida je najlošija plus-hibrid kombinacija pokazala smanjenu vrednost ispitivanog svojstva za čak 12,18% u odnosu na fertilnog analoga (P=0,01)

Devet ciklusa masovne selekcije na povećani sadržaj ulja u dve sintetičke populacije kukuruza (Zea mays L.)

The objectives of this study were to estimate changes in oil content and grain yield in the maize populations DS7u and YuSSSu. As estimations were performed at CO and C9 for both populations, it was possible to observe changes occurring following long-term mass selection for high oil content. The synthetic population DS7u was developed by recombination of 29 inbreeds of Yugoslav, Canadian and US origin. The synthetic population YuSSSu is an Iowa Stiff Stalk Synthetic - BSS(R)C5. The following statistical and genetic parameters were analyzed: means, frequency distribution, components of genetic variance and heritability. Progenies were derived according to the North Carolina Design II. Results were indicated that nine cycles of selection led to statistically significant increase in oil content and statistically significant decrease for grain yield in both populations. Estimates of additive and dominance variances for grain oil content were highly significant in C0 and C9 of the population DS7u. Dominance variance showed significance in the initial cycle of the population YuSSSu, but it disappeared in the course of nine cycles of mass selection. Additive and dominance variances for grain yield were highly significant in both initial populations. Loss of significance did not result from selection, while the proportion of dominance vs. additive variance became greater. High narrow-sense heritability was detected for grain yield and oil content in the initial cycles of both populations. Mass selection resulted in increased heritability for oil content in the DS7u population.Cilj ovog istraživanja je bio da se utvrde promene u sadržaju ulja i prinosu zrna u populacijama kukuruza DS7u i YuSSSu. Pošto su procene parametara rađene za CO i C9 za obe populacije, moguće je pratiti promene tokom dugoročne masovne selekcije za visok sadržaj ulja. Sintetička populacija DS7u dobijena je rekombinacijom 29 inbred linija jugoslavenskog, kanadskog i američkog porekla. Sintetička populacija YuSSSu je Iowa Stiff Stalk Synthetic -BSSSC5- Familije su dobijene prema North Carolina Design II modelu. Rezultati su pokazali daje 9 ciklusa selekcije dovelo do statistički značajnog povećanja sadržaja ulja i statistički značajnog smanjenja prinosa zrna u obe populacije. Aditivna i dominantna varijansa za sadržaj ulja su visoko značajne u CO i C9 populacije DS7u. Dominantna varijansa je značajna u početnom ciklusu populacije YuSSSu, ali je ova značajnost nestala posle devet ciklusa masovne selekcije. Aditivna i dominantna varijansa za prinos zrna su visoko značajne u obe početne populacije. Gubitak značajnosti nije nastao usled selekcije, dok se odnos dominantne prema aditivnoj varijansi povećao. Visoka heritabilnost u užem smislu dobijena je za prinos zrna i sadržaj ulja u početnom ciklusu obe populacije. Masovna selekcija dovela je do povećanja heritabilnosti za sadržaj ulja u populaciji DS7u

Genetičke korelacije za važnija agronomska svojstva između S1 i HS familija u dva kompozita kukuruza (Zea mays L.)

Genetic correlations for more important agronomic traits between S1 and HS families of two composites of maize (Composite B and M) were investigated. For the grain yield genotypic correlation between S1 and HS families in Comp. B was significant, medium strong (0,359*), but too low for predictable purposes. Greater correlation for the grain yield was obtained in Comp. M, it was strong and highly significant (0,663**). Correlations for grain yield were lower than for the other investigated traits in both composites. For the ear height and 1000 kernel weight these correlations were higher in Comp. B, while for the rest of the traits they were greater in Comp. M, indicating greater share of the additive in the total genotypic variance in this composite. As far as the grain yield is concerned, combined S1-HS selection should be applied only in the Comp. M, based on the strong, highly significant correlation between investigated families. In Comp. B this is not the case. For this composite, alternative procedure, for the simultaneous improvement of the population per se and its combining ability, is first to apply S1, and after that HS selection, but with very low intensity (about 30%).U radu su proučavane genetičke korelacije važnijih agronomskih svojstava između S1 i HS familija u dva kompozita kukuruza (Kotnp. B i M). Za prinos zrna, genetička korelacija S1 i HS familija u Kotnp. B bila je značajna, srednje jaka (0,359*), ali i suviše niska da bi se sa sigurnošću, na osnovu prinosa S1 familije, sudilo o njenoj KS, ili obrnuto. Veća korelacija za prinos zrna utvrđena je u Komp. M i bila je jaka i visoko značajna (0.663**). Korelacije za prinos zrna bile su niže nego za druge ispitivane osobine u oba kompozita. Za visinu klipa i masu 1000zrna vrednosti ovih korelacija bile su veće u Komp. B, a za sve ostale osobine u Komp. M, pa je verovatno u Kompozitu M udeo aditivne u ukupnoj genetičkoj varijansi veći za većinu ispitivanih svojstava. Što se tiče prinosa zrna, kombinovana S1-HS selekcija bi se smela primeniti samo u Komp. M, na osnovu visoko značajne korelacije za ovu osobinu između ispitivanih tipova familija. U Komp. B ovo ne bi smelo da se uradi. Ovde je alternativa, za istovremenu popravku prinosa populacije per se i njene KS, naizmenična primena S1 i HS selekcije (najpre S1 a zatim HS), ali uz vrlo nizak intenzitet (oko 30%)

Crveni ZP hibridi kukuruza

The following three red kernel maize genotypes were observed: the variety ZP Rumenka and two commercial hybrids ZPH-1 red (FAO 350) and ZPH-2 red (FAO 600). The aim of the study was to determine possible superiority of commercial red maize hybrids over the variety Rumenka in order to produce functional food, i.e. specific flour from red maize. The hybrid ZPH-1 red was the best in the trial with the highest antioxidant activity and anthocyaninins content, as well as, with the highest grain yield. Therefore this hybrid is very suitable for the production of maize for special purposes.U radu su ispitivana tri genotipa kukuruza crvenog zrna: sorta ZP Rumenka i dva komercijalna hibrida, ZPH-1 crveni (FAO 350) i ZPH-2 crveni (FAO 600). Svrha je bila utvrđivanje eventualne superiornosti komercijalnih crvenih hibrida u odnosu na sortu crvenog zrna za dobijanje tzv. funkcionalne hrane, odnosno specifičnog brašna od crvenog kukuruza. Kao najpogodniji genotip pokazao se ZPH-1 crveni, sa daleko najvećom antioksidativnom aktivnošću i sadržajem antocijaninina, kao i najvećim prinosom zrna. Stoga je on veoma pogodan za proizvodnju kukuruza za specifične namene

Hibridi kukuruza specifičnih svojstava za industrijsku preradu

Extremely high genetic variability of maize provides the possibility to develop new types for various purposes in the process of selection. By the method of selection, the grain composition can be significantly modified in respect to quantity and quality of certain components. It enables "designing" of new maize types with specific traits necessary for particular purposes. Maize is mainly grown as an energetic crop, but there is a broad use of types with specific traits such as: sweet corn, popcorn and white-seeded maize. Maize types with specific traits require remarkable attention not only in the process of breeding and seed production, but also in the commercial production and processing.Izuzetno velika genetička varijabilnost kukuruza omogućava da se u procesu selekcije razvijaju tipovi za raznovrsne namene. Kompozicija zrna se selekcijom može značajno menjati u pogledu količine i kvaliteta pojedinih sastavnih delova. To omogućava da se u određenim granicama "dizajniraju" novi tipovi specifičnih svojstava potrebnih za određene namene. Kukuruz se gaji prvenstveno kao energetski usev, ali se dosta široko koriste i različiti specifični tipovi kao što su: šećerci, kokičari i kukuruz belog zrna. Kod specifičnih tipova kukuruza zastupljene su osobine koje zahtevaju posebnu pažnju, kako u toku procesa oplemenjivanja tako i u procesu proizvodnje semena, u komercijalnoj proizvodnji i tokom procesa prerade

Linije kukuruza L-70/9 i B-73Rfc nose gen Rf4 na istom lokusu

The inheritance of the fertility restoration in cms-C in maize is very complex, and there are many contradictory results in the literature. There is an indication that there are different parallel or duplicated systems some of which include at least three complementary dominant genes - Rf4, Rf5, Rf6. Also, there is an indication of the duplication of the gene Rf4, which was tested in this work. We used three lines of different genetic constitution regarding Rf genes (B-73Bbms, L-70/9 and B-73cmsC-RfC) and produced different genotypes, combinations of genotypes and generations between them for the screening of fertility/sterility. The results of this work are negative, i.e. we did not prove the duplication of the Rf4 gene. This may be because of the relatively small number of lines that we have used (only three), or because there really is no duplication of the investigated gene.Nasleđivanje povraćaja fertilnosti kod cms-C tipa muške sterilnosti kukuruza je po svoj prilici veoma složeno. Smatra se da postoji više paralelnih genetičkih sistema, koji mogu biti i duplikacije istih, od kojih su neki sistemi od bar tri komplementarna dominantna gena. Postoji indikacija o duplikaciji gena Rf4 (Sisco, 1991), koja je testirana u ovom radu uz pomoć linija L-70/9 i B-73RfC, koje obe poseduju dominantan alel Rf4. Naši rezultati su negativni, odnosno duplikacija ovog gena u našem radu nije potvrđena

Kvalitet zrna i mogućnosti korišćenja ZP hibrida kukuruza

The study presents results of a long-term scientific and research work on the improvement of maize utilization at the Department of Technology of the Maize Research Institute, Zemun Polje. This research was aimed at the development of new assortment of plant food, feed and maize-based technical products of high quality. The application of modern processing technologies resulted in the production of highly valuable food: whole grain of micronised red-, yellow- and white- maize, concentrate for bread production bread and traditional Serbian corn bread with biologically valuable ingredients of red- and yellow- maize. Besides these food products, the original technology of maize cob processing has been developed. Various lignocelluloses granules intended for utilization in different sectors of industry, agriculture and environmental protection.U radu su prikazani rezultati višegodišnjeg naučnoistraživačkog rada na unapređenju korišćenja kukuruza u Odseku za tehnološka istraživanja Instituta za kukuruz. Ova istraživanja usmerena su ka stvaranju novog asortimana kvalitetne biljne hrane i tehničkih proizvoda. Primenom savremenih tehnologija prerade osvojena je proizvodnja nove i jedinstvene visokovredne hrane: integralno brašno mikronizovanog zrna crvenog, žutog i belog kukuruza, koncentrat za proizvodnju hleba, hleb i proja sa biološki vrednim sastojcima kukuruza crvenog i žutog zrna. Pored toga, razrađena je originalna tehnologija prerade kukuruznog oklaska. Dobijeni su različiti lignocelulozni granulati namenjeni za upotrebu u različitim granama industrije, poljoprivredi i zaštiti životne sredine