Sorta facelije NS priora za proizvodnju biomase u cilju dobijanja voluminozne stočne hrane

California bluebell (Phacelia tanacetifolia Benth) is a hardy plant forage. It is grown for seed production, for obtaining green forage, silage, hay, as well as crop protection, crop of green fertilizers and as bee pasture. The experiment the new variety California bluebell, NS Priora, was carried out in four replications on plots of the Institute of Field and Vegetable Crops in Backi Petrovac (φN 45 ° 20 ', λE 19 ° 40', m.s.l. 89), in 2016. Cellulose content in the grain of variety NS Priora was 4.91%, while the average yield of raw biomass was 25,000 kg ha-1. NS Priora has achieved high yields of raw biomass and good grain quality and proved to be a good feedstock for the production of seeds and roughage.Facelija (Phacelia tanacetifolia Benth) je jednogodišnja krmna biljka. Koristi se za proizvodnju semena, za dobijanje zelene krme, silaže, sena, kao zaštitni usev, usev za zelenišno đubrenje i kao pčelinja paša. Ogled sa novom sortom facelije, NS Priora, izveden je u četiri ponavljanja na parcelama Instituta za ratarstvo i povrtarstvo u Bačkom Petrovcu (φN 45°20', λE 19°40', m.s.l. 89), u 2016. godini. Sadržaj celuloze u zrnu sorte NS Priora iznosio je 4,91% dok je prosečan prinos sirove biomase iznosio 25.000 kg ha-1. NS Priora je ostvarila visoke prinose sirove biomase i dobar kvalitet zrna i pokazala se kao dobra sirovina za proizvodnju semena i voluminozne stočne hrane

An investigation of influence of solvent on the degradation kinetics of carotenoids in oil extracts of Calendula officinalis

The stability of carotenoids was studied in marigold oil extracts prepared with following solvents: Myritol 312®, paraffin oil, almond oil, olive oil, sunflower oil, grape seed oil, and soybean oil. The concentration of the carotenoids was determined by spectroscopic measurement at 450 nm. Degradation rate showed a first order dependence on the concentration of carotenoids with a faster first stage (which lasted 3550 days, depending on the solvent) and a slower second stage. The highest degradation rates were observed in extracts prepared with linoleic acid rich solvents (sunflower oil, soybean oil and grape seed oil), while the lowest were found in oil with saturated fatty acids (Myritol 312®) and paraffin oil. These results confirm the connection between the degradation of carotenoids and lipid autoxidation, and suggest that the influence of the oil solvents on the stability of oil extracts of Calendula officinalis is a factor that must be considered when selecting a solvent for the production of marigold oil extracts

Vehicle-Controlled Effect of Urea on Normal and SLS – Irritated Skin

It is known that, depending on the concentration, treatment with urea could improve skin barrier function, despite its penetration-enhancing properties. This controversial skin effect of urea has been explored systematically in this study in terms of the effect of vehicle on the performance of urea. In the first part, a series of four semi-solid emulsions with 5% (w/w) urea, varying in the type of emulsion, nature of emulsifier and polarity of oil ingredients, have been evaluated with regard to their skin hydrating and transepidermal water loss (TEWL)-modifying properties. Placebo samples were tested alongside the urea-containing ones. Two best performing moisturisers from the above were chosen for the second part of the study, in which sodium lauryl sulphate (SLS)-irritated skin was treated with both placebo and urea-containing samples. In addition to TEWL and skin hydration level, the erythema index (EI) was measured before, during and after the treatment. The results have shown that barrier-improving and hydrating abilities of urea are bi-directional and dependent on both the type of vehicle used for its delivery and the state of skin

Synthesis and characterization of thermosensitive hydrogels and the investigation of modified release of ibuprofen

The method of the synthesis of poly(N-isopropylacrylamide-co-2-hydroxypropyl methacrylate) hydrogels obtained by radical polymerization is described. Their characterization was carried out by the determination of the quantity of residual monomers and by investigating their structure using FTIR. Three glass transitions were detected by DSC method. The porous surfaces of hydrogels with incorporated ibuprofen were shown in SEM micrographs. The swelling ratio of hydrogels decreased with the temperature increase and the swelling transport mechanism changed from non-Fickian to Fickian. Ibuprofen was incorporated in the hydrogel as a drug carrier and the released quantity depending on the temperature was monitored by HPLC. The hydrogel with the lower cross-linker content had the highest swelling degree (α = 34.72) at 10°C and released the largest amount of ibuprofen (64.21 mg/gxerogel) at 40C.Prikazan je metod sinteze hidrogelova poli(N-izopropilakrilamid-ko-2-hidroksi-propilmetakrilata), p(NIPAM-ko-HPMet), sa 5 mol% monomera HPMet-a dobijenih radikalnom polimerizacijom. Karakterizacija sintetisanih hidrogelova je izvedena određivanjem količine rezidualnih monomera i izvršena je njihova strukturna analiza pomoću infracrvene spektroskopije (FTIR), diferencijalne skenirajuće kalorimetrije (DSC) i skenirajuće elektronske mikroskopije (SEM). FTIR spektri sintetisanih hidrogelova p(NIPAM-ko-HPMet)-a sa različitim sadržajem umreživača EGDM-a pokazuju uzajamnu sličnost u strukturi. Površinska struktura liofilizovanih hidrogelova je porozna i uočavaju se čestice uklopljenog ibuprofena. Termička svojstva kserogelova p(NIPAM-ko-HPMet)-a pokazuju tri staklasta prelaza sa endotermnim pikovima slabog intenziteta. Sadržaj rezidualnih monomera dovodi do zaključka da je konverzija monomera tokom sinteze gotovo kompletna. Kine-tika bubrenja hidrogelova pokazuje da se pri povećanju temperature od 20 na 40 °C mehanizam transporta tečnosti menja iz ne-Fikovog (proces bubrenja kontroliše difuzija tečnosti i relaksacija polimernih lanaca) u Fikov (kontrolisan samo difuzijom tečnosti). Mogućnost primene hidrogelova p(NIPAM-ko-HPMet)-a kao nosača lekova ispitivana je u zavisnosti od temperature. Ibuprofen, korišćen kao model lekovita supstanca, uklopljen je u hidrogel i količina oslobođene aktivne supstance određena je HPLC metodom. Utvrđeno je da uzorak hidrogela p(NIPAM-ko-HPMet)-a sa sadržajem umreživača od 1 mol% EGDM-a dostiže najveći stepen bubrenja (α = 34,72) na 10°C i otpušta najveću količinu ibuprofena na 40 °C (64,21 mg/gkserogel). Ispitana svojstva predstavljaju dobru osnovu za potencijalnu primenu hidrogelova kao nosača lekovitih supstanci sa modifikovanim oslobađanjem

Sorta facelije NS priora za proizvodnju biomase u cilju dobijanja voluminozne stočne hrane

California bluebell (Phacelia tanacetifolia Benth) is a hardy plant forage. It is grown for seed production, for obtaining green forage, silage, hay, as well as crop protection, crop of green fertilizers and as bee pasture. The experiment the new variety California bluebell, NS Priora, was carried out in four replications on plots of the Institute of Field and Vegetable Crops in Backi Petrovac (φN 45 ° 20 ', λE 19 ° 40', m.s.l. 89), in 2016. Cellulose content in the grain of variety NS Priora was 4.91%, while the average yield of raw biomass was 25,000 kg ha-1. NS Priora has achieved high yields of raw biomass and good grain quality and proved to be a good feedstock for the production of seeds and roughage.Facelija (Phacelia tanacetifolia Benth) je jednogodišnja krmna biljka. Koristi se za proizvodnju semena, za dobijanje zelene krme, silaže, sena, kao zaštitni usev, usev za zelenišno đubrenje i kao pčelinja paša. Ogled sa novom sortom facelije, NS Priora, izveden je u četiri ponavljanja na parcelama Instituta za ratarstvo i povrtarstvo u Bačkom Petrovcu (φN 45°20', λE 19°40', m.s.l. 89), u 2016. godini. Sadržaj celuloze u zrnu sorte NS Priora iznosio je 4,91% dok je prosečan prinos sirove biomase iznosio 25.000 kg ha-1. NS Priora je ostvarila visoke prinose sirove biomase i dobar kvalitet zrna i pokazala se kao dobra sirovina za proizvodnju semena i voluminozne stočne hrane

Inorganically modified diatomite as a potential prolonged-release drug carrier

Inorganic modification of diatomite was performed with the precipitation product of partially neutralized aluminum sulfate solution at three different mass ratios. The starting and the modified diatomites were characterized by SEM-EDS, FTIR, thermal analysis and zeta potential measurements and evaluated for drug loading capacity in adsorption batch experiments using diclofenac sodium (DS) as a model drug. In vitro drug release studies were performed in phosphate buffer pH 6.8 from comprimates containing: the drug adsorbed onto the selected modified diatomite sample (DAMD), physical mixture of the drug with the selected modified diatomite sample (PMDMD) and physical mixture of the drug with the starting diatomite (PMDD). In vivo acute toxicity testing of the modified diatomite samples was performed on mice. High adsorbent loading of the selected modified diatomite sample (similar to 250 mg/g in 2 h) enabled the preparation of comprimates containing adsorbed DS in the amount near to its therapeutic dose. Drug release studies demonstrated prolonged release of DS over a period of 8 h from both DAMD comprimates (18% after 8 h) and PMDMD comprimates (45% after 8 h). The release kinetics for DAMD and PMDMD comprimates fitted well with Korsmeyer-Peppas and Bhaskar models, indicating that the release mechanism was a combination of non-Fickian diffusion and ion exchange process

Experimental Design in Formulation of Diazepam Nanoemulsions: Physicochemical and Pharmacokinetic Performances

With the aid of experimental design, we developed and characterized nanoemulsions for parenteral drug delivery. Formulations containing a mixture of medium-chain triglycerides and soybean oil as oil phase, lecithin (soybean/egg) and polysorbate 80 as emulsifiers, and 0.1M phosphate buffer solution (pH 8) as aqueous phase were prepared by cold high-pressure homogenization. To study the effects of the oil content, lecithin type, and the presence of diazepam as a model drug and their interactions on physicochemical characteristics of nanoemulsions, a three factor two-level full factorial design was applied. The nanoemulsions were evaluated concerning droplet size and size distribution, surface charge, viscosity, morphology, drug-excipient interactions, and physical stability. The characterization revealed the small spherical droplets in the range 195-220nm with polydispersity index below 0.15 and zeta potential between -30 and -60mV. Interactions among the investigated factors, rather than factors alone, were shown to more profoundly affect nanoemulsion characteristics. In vivo pharmacokinetic study of selected diazepam nanoemulsions with different oil content (20%, 30%, and 40%, w/w) demonstrated fast and intense initial distribution into rat brain of diazepam from nanoemulsions with 20% and 30% (w/w) oil content, suggesting their applicability in urgent situations