Identification of novel chondroitin proteoglycans in Caenorhabditis elegans: embryonic cell division depends on CPG-1 and CPG-2.

Vertebrates produce multiple chondroitin sulfate proteoglycans that play important roles in development and tissue mechanics. In the nematode Caenorhabditis elegans, the chondroitin chains lack sulfate but nevertheless play essential roles in embryonic development and vulval morphogenesis. However, assignment of these functions to specific proteoglycans has been limited by the lack of identified core proteins. We used a combination of biochemical purification, Western blotting, and mass spectrometry to identify nine C. elegans chondroitin proteoglycan core proteins, none of which have homologues in vertebrates or other invertebrates such as Drosophila melanogaster or Hydra vulgaris. CPG-1/CEJ-1 and CPG-2 are expressed during embryonic development and bind chitin, suggesting a structural role in the egg. RNA interference (RNAi) depletion of individual CPGs had no effect on embryonic viability, but simultaneous depletion of CPG-1/CEJ-1 and CPG-2 resulted in multinucleated single-cell embryos. This embryonic lethality phenocopies RNAi depletion of the SQV-5 chondroitin synthase, suggesting that chondroitin chains on these two proteoglycans are required for cytokinesis

The incorporation of matter into characteristic numerical relativity

A code that implements Einstein equations in the characteristic formulation in 3D has been developed and thoroughly tested for the vacuum case. Here, we describe how to incorporate matter, in the form of a perfect fluid, into the code. The extended code has been written and validated in a number of cases. It is stable and capable of contributing towards an understanding of a number of problems in black hole astrophysics.Comment: 15 pages + 4 (eps) figure

Review of risperidone for the treatment of pediatric and adolescent bipolar disorder and schizophrenia

Risperidone is a commonly used medication for the treatment of bipolar disorder and schizophrenia in children and adolescents. It has been studied as a monotherapy treatment in early onset schizophrenia and as both monotherapy and combination therapy for pediatric bipolar disorder. Studies to date indicate that risperidone is an effective treatment for positive and negative symptoms of schizophrenia and mania symptoms of bipolar disorder. In young patient populations, side effects such as weight gain, extrapyramidal side effects, and prolactin elevation require consideration when evaluating the risk benefit ratio for individual patients. Here we review published studies of risperidone for the treatment of bipolar disorder and schizophrenia in children and adolescents to provide practitioners with an overview of published data on the efficacy and safety of risperidone in these patient populations

Precision Pharmacotherapy Enables Precision Medicine

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/138280/1/phar1998_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/138280/2/phar1998.pd

High-powered Gravitational News

We describe the computation of the Bondi news for gravitational radiation. We have implemented a computer code for this problem. We discuss the theory behind it as well as the results of validation tests. Our approach uses the compactified null cone formalism, with the computational domain extending to future null infinity and with a worldtube as inner boundary. We calculate the appropriate full Einstein equations in computational eth form in (a) the interior of the computational domain and (b) on the inner boundary. At future null infinity, we transform the computed data into standard Bondi coordinates and so are able to express the news in terms of its standard $N_{+}$ and $N_{\times}$ polarization components. The resulting code is stable and second-order convergent. It runs successfully even in the highly nonlinear case, and has been tested with the news as high as 400, which represents a gravitational radiation power of about $10^{13}M_{\odot}/sec$.Comment: 24 pages, 4 figures. To appear in Phys. Rev.

Gravitational Waves from a Fissioning White Hole

We present a fully nonlinear calculation of the waveform of the gravitational radiation emitted in the fission of a vacuum white hole. At early times, the waveforms agree with close-approximation perturbative calculations but they reveal dramatic time and angular dependence in the nonlinear regime. The results pave the way for a subsequent computation of the radiation emitted after a binary black hole merger.Comment: 11 pages, 6 figures, RevTeX

On characteristic initial data for a star orbiting a black hole

We take further steps in the development of the characteristic approach to enable handling the physical problem of a compact self-gravitating object, such as a neutron star, in close orbit around a black hole. We examine different options for setting the initial data for this problem and, in order to shed light on their physical relevance, we carry out short time evolution of this data. To this end we express the matter part of the characteristic gravity code so that the hydrodynamics are in conservation form. The resulting gravity plus matter relativity code provides a starting point for more refined future efforts at longer term evolution. In the present work we find that, independently of the details of the initial gravitational data, the system quickly flushes out spurious gravitational radiation and relaxes to a quasi-equilibrium state with an approximate helical symmetry corresponding to the circular orbit of the star.Comment: 20 pages, 10 figure

Cauchy boundaries in linearized gravitational theory

We investigate the numerical stability of Cauchy evolution of linearized gravitational theory in a 3-dimensional bounded domain. Criteria of robust stability are proposed, developed into a testbed and used to study various evolution-boundary algorithms. We construct a standard explicit finite difference code which solves the unconstrained linearized Einstein equations in the 3+1 formulation and measure its stability properties under Dirichlet, Neumann and Sommerfeld boundary conditions. We demonstrate the robust stability of a specific evolution-boundary algorithm under random constraint violating initial data and random boundary data.Comment: 23 pages including 3 figures and 2 tables, revte

Harmonic Initial-Boundary Evolution in General Relativity

Computational techniques which establish the stability of an evolution-boundary algorithm for a model wave equation with shift are incorporated into a well-posed version of the initial-boundary value problem for gravitational theory in harmonic coordinates. The resulting algorithm is implemented as a 3-dimensional numerical code which we demonstrate to provide stable, convergent Cauchy evolution in gauge wave and shifted gauge wave testbeds. Code performance is compared for Dirichlet, Neumann and Sommerfeld boundary conditions and for boundary conditions which explicitly incorporate constraint preservation. The results are used to assess strategies for obtaining physically realistic boundary data by means of Cauchy-characteristic matching.Comment: 31 pages, 14 figures, submitted to Physical Review

Complete null data for a black hole collision

We present an algorithm for calculating the complete data on an event horizon which constitute the necessary input for characteristic evolution of the exterior spacetime. We apply this algorithm to study the intrinsic and extrinsic geometry of a binary black hole event horizon, constructing a sequence of binary black hole event horizons which approaches a single Schwarzschild black hole horizon as a limiting case. The linear perturbation of the Schwarzschild horizon provides global insight into the close limit for binary black holes, in which the individual holes have joined in the infinite past. In general there is a division of the horizon into interior and exterior regions, analogous to the division of the Schwarzschild horizon by the r=2M bifurcation sphere. In passing from the perturbative to the strongly nonlinear regime there is a transition in which the individual black holes persist in the exterior portion of the horizon. The algorithm is intended to provide the data sets for production of a catalog of nonlinear post-merger wave forms using the PITT null code.Comment: Revised version, to appear in Phys. Rev. D. July 15 (2001), 41 pages, 11 figures, RevTeX/epsf/psfi