693 research outputs found
Population dynamics and harvest management of eastern mallards
Managing sustainable harvest of wildlife populations requires regular collection of demographic data and robust estimates of demographic parameters. Estimates can then be used to develop a harvest strategy to guide decisionâmaking. Mallards (Anas platyrhynchos) are an important species in the Atlantic Flyway for many users and they exhibited exponential growth in the eastern United States between the 1970s and 1990s. Since then, estimates of mallard abundance have declined 16%, prompting the Atlantic Flyway Council and United States Fish and Wildlife Service to implement more restrictive hunting regulations and develop a new harvest strategy predicated on an updated population model. Our primary objective was to develop an integrated population model (IPM) for use in an eastern mallard harvest management strategy. We developed an IPM using annual estimates of breeding abundance, 2âseason banding and recovery data, and hunterharvest data from 1998 to 2018.When developing the model, we used novel model selection methods to test various forms of a submodel for survival including estimating the degree of harvest additivity and any ageâspecific trends. The top survival subâmodel included a negative annual trend on juvenile survival. The IPM posterior estimates for population abundance tracked closely with the observed estimates and estimates of mean annual population growth rate ranged from 0.88 to 1.08. Our population model provided increased precision in abundance estimates compared to survey methods for use in an updated harvest strategy. The IPM
posterior estimates of survival rates were relatively stable for adult cohorts, and annual growth rate was positively correlated with the female age ratio, a measure of reproduction. Either or both of those demographic parameters, juvenile survival or reproduction, could be a target for management efforts to address the population decline. The resulting demographic parameters provided information on the equilibrium population size and can be used in an adaptive harvest strategy for mallards in eastern North America
Population dynamics and harvest management of eastern mallards
Managing sustainable harvest of wildlife populations requires regular collection of demographic data and robust estimates of demographic parameters. Estimates can then be used to develop a harvest strategy to guide decisionâmaking. Mallards (Anas platyrhynchos) are an important species in the Atlantic Flyway for many users and they exhibited exponential growth in the eastern United States between the 1970s and 1990s. Since then, estimates of mallard abundance have declined 16%, prompting the Atlantic Flyway Council and United States Fish and Wildlife Service to implement more restrictive hunting regulations and develop a new harvest strategy predicated on an updated population model. Our primary objective was to develop an integrated population model (IPM) for use in an eastern mallard harvest management strategy. We developed an IPM using annual estimates of breeding abundance, 2âseason banding and recovery data, and hunterharvest data from 1998 to 2018.When developing the model, we used novel model selection methods to test various forms of a submodel for survival including estimating the degree of harvest additivity and any ageâspecific trends. The top survival subâmodel included a negative annual trend on juvenile survival. The IPM posterior estimates for population abundance tracked closely with the observed estimates and estimates of mean annual population growth rate ranged from 0.88 to 1.08. Our population model provided increased precision in abundance estimates compared to survey methods for use in an updated harvest strategy. The IPM posterior estimates of survival rates were relatively stable for adult cohorts, and annual growth rate was positively correlated with the female age ratio, a measure of reproduction. Either or both of those demographic parameters, juvenile survival or reproduction, could be a target for management efforts to address the population decline. The resulting demographic parameters provided information on the equilibrium population size and can be used in an adaptive harvest strategy for mallards in eastern North America
Biodiversity and Ecosystem Function in the Gulf of Maine: Pattern and Role of Zooplankton and Pelagic Nekton
This paper forms part of a broader overview of biodiversity of marine life in the Gulf of Maine area (GoMA), facilitated by the GoMA Census of Marine Life program. It synthesizes current data on species diversity of zooplankton and pelagic nekton, including compilation of observed species and descriptions of seasonal, regional and cross-shelf diversity patterns. Zooplankton diversity in the GoMA is characterized by spatial differences in community composition among the neritic environment, the coastal shelf, and deep offshore waters. Copepod diversity increased with depth on the Scotian Shelf. On the coastal shelf of the western Gulf of Maine, the number of higher-level taxonomic groups declined with distance from shore, reflecting more nearshore meroplankton. Copepod diversity increased in late summer, and interdecadal diversity shifts were observed, including a period of higher diversity in the 1990s. Changes in species diversity were greatest on interannual scales, intermediate on seasonal scales, and smallest across regions, in contrast to abundance patterns, suggesting that zooplankton diversity may be a more sensitive indicator of ecosystem response to interannual climate variation than zooplankton abundance. Local factors such as bathymetry, proximity of the coast, and advection probably drive zooplankton and pelagic nekton diversity patterns in the GoMA, while ocean-basin-scale diversity patterns probably contribute to the increase in diversity at the Scotian Shelf break, a zone of mixing between the cold-temperate community of the shelf and the warm-water community offshore. Pressing research needs include establishment of a comprehensive system for observing change in zooplankton and pelagic nekton diversity, enhanced observations of underknown\u27\u27 but important functional components of the ecosystem, population and metapopulation studies, and development of analytical modeling tools to enhance understanding of diversity patterns and drivers. Ultimately, sustained observations and modeling analysis of biodiversity must be effectively communicated to managers and incorporated into ecosystem approaches for management of GoMA living marine resources
Detection of postoperative granulation tissue with an ICG-enhanced integrated OI-/X-ray System
<p>Abstract</p> <p>Background</p> <p>The development of postoperative granulation tissue is one of the main postoperative risks after lumbar spine surgery. This granulation tissue may lead to persistent or new clinical symptoms or complicate a follow up surgery. A sensitive non-invasive imaging technique, that could diagnose this granulation tissue at the bedside, would help to develop appropriate treatments. Thus, the purpose of this study was to establish a fast and economic imaging tool for the diagnosis of granulation tissue after lumbar spine surgery, using a new integrated Optical Imaging (OI)/X-ray imaging system and the FDA-approved fluorescent contrast agent Indocyanine Green (ICG).</p> <p>Methods</p> <p>12 male Sprague Dawley rats underwent intervertebral disk surgery. Imaging of the operated lumbar spine was done with the integrated OI/X-ray system at 7 and 14 days after surgery. 6 rats served as non-operated controls. OI/X-ray scans of all rats were acquired before and after intravenous injection of the FDA-approved fluorescent dye Indocyanine Green (ICG) at a dose of 1 mg/kg or 10 mg/kg. The fluorescence signal of the paravertebral soft tissues was compared between different groups of rats using Wilcoxon-tests. Lumbar spines and paravertebral soft tissues were further processed with histopathology.</p> <p>Results</p> <p>In both dose groups, ICG provided a significant enhancement of soft tissue in the area of surgery, which corresponded with granulation tissue on histopathology. The peak and time interval of fluorescence enhancement was significantly higher using 10 mg/kg dose of ICG compared to the 1 mg/kg ICG dose. The levels of significance were p < 0.05. Fusion of OI data with X-rays allowed an accurate anatomical localization of the enhancing granulation tissue.</p> <p>Conclusion</p> <p>ICG-enhanced OI is a suitable technique to diagnose granulation tissue after lumbar spine surgery. This new imaging technique may be clinically applicable for postoperative treatment monitoring. It could be also used to evaluate the effect of anti-inflammatory drugs and may even allow evaluations at the bedside with new hand-held OI scanners.</p
Looking for a needle in a haystack: inference about individual fitness components in a heterogeneous population
Studies of wild vertebrates have provided evidence of substantial differences in lifetime reproduction among individuals and the sequences of life history âstatesâ during life (breeding, nonbreeding, etc.). Such differences may reflect âfixedâ differences in fitness components among individuals determined before, or at the onset of reproductive life. Many retrospective life history studies have translated this idea by assuming a âlatentâ unobserved heterogeneity resulting in a fixed hierarchy among individuals in fitness components. Alternatively, fixed differences among individuals are not necessarily needed to account for observed levels of individual heterogeneity in life histories. Individuals with identical fitness traits may stochastically experience different outcomes for breeding and survival through life that lead to a diversity of âstateâ sequences with some individuals living longer and being more productive than others, by chance alone. The question is whether individuals differ in their underlying fitness components in ways that cannot be explained by observable âstatesâ such as age, previous breeding success, etc. Here, we compare statistical models that represent these opposing hypotheses, and mixtures of them, using data from kittiwakes. We constructed models that accounted for observed covariates, individual random effects (unobserved heterogeneity), first-order Markovian transitions between observed states, or combinations of these features. We show that individual sequences of states are better accounted for by models incorporating unobserved heterogeneity than by models including first-order Markov processes alone, or a combination of both. If we had not considered individual heterogeneity, models including Markovian transitions would have been the best performing ones. We also show that inference about age-related changes in fitness components is sensitive to incorporation of underlying individual heterogeneity in models. Our approach provides insight into the sources of individual heterogeneity in life histories, and can be applied to other data sets to examine the ubiquity of our results across the tree of life
Next-generation sequencing identifies the natural killer cell microRNA transcriptome
Natural killer (NK) cells are innate lymphocytes important for early host defense against infectious pathogens and surveillance against malignant transformation. Resting murine NK cells regulate the translation of effector molecule mRNAs (e.g., granzyme B, GzmB) through unclear molecular mechanisms. MicroRNAs (miRNAs) are small noncoding RNAs that post-transcriptionally regulate the translation of their mRNA targets, and are therefore candidates for mediating this control process. While the expression and importance of miRNAs in T and B lymphocytes have been established, little is known about miRNAs in NK cells. Here, we used two next-generation sequencing (NGS) platforms to define the miRNA transcriptomes of resting and cytokine-activated primary murine NK cells, with confirmation by quantitative real-time PCR (qRT-PCR) and microarrays. We delineate a bioinformatics analysis pipeline that identified 302 known and 21 novel mature miRNAs from sequences obtained from NK cell small RNA libraries. These miRNAs are expressed over a broad range and exhibit isomiR complexity, and a subset is differentially expressed following cytokine activation. Using these miRNA NGS data, miR-223 was identified as a mature miRNA present in resting NK cells with decreased expression following cytokine activation. Furthermore, we demonstrate that miR-223 specifically targets the 3âČ untranslated region of murine GzmB in vitro, indicating that this miRNA may contribute to control of GzmB translation in resting NK cells. Thus, the sequenced NK cell miRNA transcriptome provides a valuable framework for further elucidation of miRNA expression and function in NK cell biology
Crystalline Color Superconductivity
In any context in which color superconductivity arises in nature, it is
likely to involve pairing between species of quarks with differing chemical
potentials. For suitable values of the differences between chemical potentials,
Cooper pairs with nonzero total momentum are favored, as was first realized by
Larkin, Ovchinnikov, Fulde and Ferrell (LOFF). Condensates of this sort
spontaneously break translational and rotational invariance, leading to gaps
which vary periodically in a crystalline pattern. Unlike the original LOFF
state, these crystalline quark matter condensates include both spin zero and
spin one Cooper pairs. We explore the range of parameters for which crystalline
color superconductivity arises in the QCD phase diagram. If in some shell
within the quark matter core of a neutron star (or within a strange quark star)
the quark number densities are such that crystalline color superconductivity
arises, rotational vortices may be pinned in this shell, making it a locus for
glitch phenomena.Comment: 40 pages, LaTeX with eps figs. v2: New paragraph on Ginzburg-Landau
treatment of LOFF phase in section 5. References added. v3: Small changes
only. Version to appear in Phys. Rev.
Daptomycin versus standard therapy for bacteremia and endocarditis caused by Staphylococcus aureus.
BACKGROUND: Alternative therapies for Staphylococcus aureus bacteremia and endocarditis are needed.
METHODS: We randomly assigned 124 patients with S. aureus bacteremia with or without endocarditis to receive 6 mg of daptomycin intravenously per kilogram of body weight daily and 122 to receive initial low-dose gentamicin plus either an antistaphylococcal penicillin or vancomycin. The primary efficacy end point was treatment success 42 days after the end of therapy.
RESULTS: Forty-two days after the end of therapy in the modified intention-to-treat analysis, a successful outcome was documented for 53 of 120 patients who received daptomycin as compared with 48 of 115 patients who received standard therapy (44.2 percent vs. 41.7 percent; absolute difference, 2.4 percent; 95 percent confidence interval, -10.2 to 15.1 percent). Our results met prespecified criteria for the noninferiority of daptomycin. The success rates were similar in subgroups of patients with complicated bacteremia, right-sided endocarditis, and methicillin-resistant S. aureus. Daptomycin therapy was associated with a higher rate of microbiologic failure than was standard therapy (19 vs. 11 patients, P=0.17). In 6 of the 19 patients with microbiologic failure in the daptomycin group, isolates with reduced susceptibility to daptomycin emerged; similarly, a reduced susceptibility to vancomycin was noted in isolates from patients treated with vancomycin. As compared with daptomycin therapy, standard therapy was associated with a nonsignificantly higher rate of adverse events that led to treatment failure due to the discontinuation of therapy (17 vs. 8, P=0.06). Clinically significant renal dysfunction occurred in 11.0 percent of patients who received daptomycin and in 26.3 percent of patients who received standard therapy (P=0.004).
CONCLUSIONS: Daptomycin (6 mg per kilogram daily) is not inferior to standard therapy for S. aureus bacteremia and right-sided endocarditis. (ClinicalTrials.gov number, NCT00093067 [ClinicalTrials.gov].)
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