Electron transport phosphorylation in rumen butyrivibrios: unprecedented ATP yield for glucose fermentation to butyrate.

From a genomic analysis of rumen butyrivibrios (Butyrivibrio and Pseudobutyrivibrio sp.), we have re-evaluated the contribution of electron transport phosphorylation (ETP) to ATP formation in this group. This group is unique in that most (76%) genomes were predicted to possess genes for both Ech and Rnf transmembrane ion pumps. These pumps act in concert with the NifJ and Bcd-Etf to form a electrochemical potential (ΔμH(+) and ΔμNa(+)), which drives ATP synthesis by ETP. Of the 62 total butyrivibrio genomes currently available from the Hungate 1000 project, all 62 were predicted to possess NifJ, which reduces oxidized ferredoxin (Fdox) during pyruvate conversion to acetyl-CoA. All 62 possessed all subunits of Bcd-Etf, which reduces Fdox and oxidizes reduced NAD during crotonyl-CoA reduction. Additionally, 61 genomes possessed all subunits of the Rnf, which generates ΔμH(+) or ΔμNa(+) from oxidation of reduced Fd (Fdred) and reduction of oxidized NAD. Further, 47 genomes possessed all six subunits of the Ech, which generates ΔμH(+) from oxidation of Fdred. For glucose fermentation to butyrate and H2, the electrochemical potential established should drive synthesis of ∼1.5 ATP by the F0F1-ATP synthase (possessed by all 62 genomes). The total yield is ∼4.5 ATP/glucose after accounting for three ATP formed by classic substrate-level phosphorylation, and it is one the highest yields for any glucose fermentation. The yield was the same when unsaturated fatty acid bonds, not H(+), served as the electron acceptor (as during biohydrogenation). Possession of both Ech and Rnf had been previously documented in only a few sulfate-reducers, was rare in other rumen prokaryotic genomes in our analysis, and may confer an energetic advantage to rumen butyrivibrios. This unique energy conservation system might enhance the butyrivibrios' ability to overcome growth inhibition by unsaturated fatty acids, as postulated herein

The sciences in America, circa 1880

For many years American science in the late 19th century was regarded as an intellectual backwater. This view derived from the assumption that the health of American science at the time was equivalent to the condition of pure science, especially pure physics. However, a closer look reveals that there was considerable vitality in American scientific research, especially in the earth and life sciences. This vitality is explainable in part by the natural scientific resources of the American continent but also in part by the energy given science from religious impulses, social reformism, and practicality. Furthermore, contrary to recent assumptions, the federal government was a significant patron of American science. The portrait of American science circa 1880 advanced in this article suggests that the nation's scientific enterprise was characterized by pluralism of institutional support and motive and that such pluralism has historically been the normal mode

INCOME REMINDER AND THE DIVERGENCE BETWEEN WILLINGNESS-TO-PAY ESTIMATES ASSOCIATED WITH DICHOTOMOUS CHOICE AND OPEN-ENDED ELICITATION FORMATS

This study investigates whether an income reminder can reduce the divergence between the willingness-to-pay (WTP) estimates associated with the open-ended (OE) and dichotomous choice (DC) elicitation formats. Results show that without an income reminder, WTP estimate associated with DC elicitation format is about 1.66 times as large as that associated with OE elicitation format. With an income reminder, the WTP estimate associated with DC format decreased while the WTP estimate associated with OE format increased, and the divergence between the WTP estimates was almost eliminated.dichotomous choice, open-ended, income reminder, water quality improvement, willingness-to-pay, Research Methods/ Statistical Methods,

Models of verbal working memory capacity: What does it take to make them work?

Theories of working memory (WM) capacity limits will be more useful when we know what aspects of performance are governed by the limits and what aspects are governed by other memory mechanisms. Whereas considerable progress has been made on models of WM capacity limits for visual arrays of separate objects, less progress has been made in understanding verbal materials, especially when words are mentally combined to form multiword units or chunks. Toward a more comprehensive theory of capacity limits, we examined models of forced-choice recognition of words within printed lists, using materials designed to produce multiword chunks in memory (e.g., leather brief case). Several simple models were tested against data from a variety of list lengths and potential chunk sizes, with test conditions that only imperfectly elicited the interword associations. According to the most successful model, participants retained about 3 chunks on average in a capacity-limited region of WM, with some chunks being only subsets of the presented associative information (e.g., leather brief case retained with leather as one chunk and brief case as another). The addition to the model of an activated long-term memory component unlimited in capacity was needed. A fixed-capacity limit appears critical to account for immediate verbal recognition and other forms of WM. We advance a model-based approach that allows capacity to be assessed despite other important processing contributions. Starting with a psychological-process model of WM capacity developed to understand visual arrays, we arrive at a more unified and complete model

Quantum logic as superbraids of entangled qubit world lines

Presented is a topological representation of quantum logic that views entangled qubit spacetime histories (or qubit world lines) as a generalized braid, referred to as a superbraid. The crossing of world lines is purely quantum in nature, most conveniently expressed analytically with ladder-operator-based quantum gates. At a crossing, independent world lines can become entangled. Complicated superbraids are systematically reduced by recursively applying novel quantum skein relations. If the superbraid is closed (e.g. representing quantum circuits with closed-loop feedback, quantum lattice gas algorithms, loop or vacuum diagrams in quantum field theory), then one can decompose the resulting superlink into an entangled superposition of classical links. In turn, for each member link, one can compute a link invariant, e.g. the Jones polynomial. Thus, a superlink possesses a unique link invariant expressed as an entangled superposition of classical link invariants.Comment: 4 page

Overview and Summary of the Third AIAA High Lift Prediction Workshop

The third AIAA CFD High-Lift Prediction Workshop was held in Denver, Colorado, in June 2017. The goals of the workshop continued in the tradition of the first and second high-lift workshops: to assess the numerical prediction capability of current-generation computational fluid dynamics (CFD) technology for swept, medium/high-aspect-ratio wings in landing/takeoff (high-lift) configurations. This workshop analyzed the flow over two different configurations, a clean high-lift version of the NASA Common Research Model, and the JAXA Standard Model. The former was a CFD-only study, as experimental data were not available prior to the workshop. The latter was a nacelle/pylon installation study that included comparison with experimental wind tunnel data. The workshop also included a 2-D turbulence model verification exercise. Thirty-five participants submitted a total of 79 data sets of CFD results. A variety of grid systems (both structured and unstructured) as well as different flow simulation methodologies (including Reynolds-averaged Navier-Stokes and Lattice-Boltzmann) were used. This paper analyzes the combined results from all workshop participants. A statistical summary of the CFD results is also included