How Good is our Kentucky Haylage? A Summary of 2017-18 Farm Results

The ability to harvest moist forage as hay gives Kentucky producers many advantages, including timely harvest, higher forage quality, and less weathering loss over hay systems. The baleage system allows producers to utilize commonly available forage equipment (mowers, rakes, balers) rather than requiring choppers and silo structures or bags. Making high quality baleage requires timely access to bale wrappers

Round Bale Silage – Farmer Results in Kentucky

The ability to make silage in round bale packages allows producers to avoid rain damage and produce high quality stored forage. The proliferation of bale wrappers (both individual and inline types) has made this technology more available to producers. Although the process for making baleage is well documented, producers have experienced poor fermentation and in some cases botulism toxicity from baleage. The parameters of good silage are well known and include pH below 5.0 and lactic acid concentrations above 3% on a DM basis. However, tests of farmer produced baleage reveals that often these target values are not achieved. To better understand the fermentation characteristics of Kentucky baleage, a survey was conducted of round bale silage samples from several Kentucky counties in 2017, 2018 and 2019. Moisture content (MC) at baling was the greatest determinant of fermentation success as measured by pH and lactic acid concentration. Moisture contents were highly variable, with more outside the recommended range of MC (40 to 60%) than within. Farmer practices including wilting time and equipment used were recorded for the 2019 samples. All of the well‐accepted practices for making baleage were confirmed in this survey (cutting on time, wilting to proper MC, dense bales, achieving and maintaining anaerobic storage conditions). Baleage samples exceeding 65% MC had elevated butyric acid concentrations, indicating secondary fermentation by clostridial bacteria

Enhancer viruses and a transgenic platform for combinatorial cell subclass-specific labeling

The rapid pace of cell type identification by new single-cell analysis methods has not been met with efficient experimental access to the newly discovered types. To enable flexible and efficient access to specific neural populations in the mouse cortex, we collected chromatin accessibility data from individual cells and clustered the single-cell data to identify enhancers specific for cell classes and subclasses. When cloned into adeno-associated viruses (AAVs) and delivered to the brain by retro-orbital injections, these enhancers drive transgene expression in specific cell subclasses in the cortex. We characterize several enhancer viruses in detail to show that they result in labeling of different projection neuron subclasses in mouse cortex, and that one of them can be used to label the homologous projection neuron subclass in human cortical slices. To enable the combinatorial labeling of more than one cell type by enhancer viruses, we developed a three-color Cre-, Flp- and Nigri- recombinase dependent reporter mouse line, Ai213. The delivery of three enhancer viruses driving these recombinases via a single retroorbital injection into a single Ai213 transgenic mouse results in labeling of three different neuronal classes/subclasses in the same brain tissue. This approach combines unprecedented flexibility with specificity for investigation of cell types in the mouse brain and beyond

Whole-exome sequencing and clinical interpretation of FFPE tumor samples to guide precision cancer medicine

Translating whole exome sequencing (WES) for prospective clinical use may impact the care of cancer patients; however, multiple innovations are necessary for clinical implementation. These include: (1) rapid and robust WES from formalin-fixed paraffin embedded (FFPE) tumor tissue, (2) analytical output similar to data from frozen samples, and (3) clinical interpretation of WES data for prospective use. Here, we describe a prospective clinical WES platform for archival FFPE tumor samples. The platform employs computational methods for effective clinical analysis and interpretation of WES data. When applied retrospectively to 511 exomes, the interpretative framework revealed a “long tail” of somatic alterations in clinically important genes. Prospective application of this approach identified clinically relevant alterations in 15/16 patients. In one patient, previously undetected findings guided clinical trial enrollment leading to an objective clinical response. Overall, this methodology may inform the widespread implementation of precision cancer medicine

Stellar Spectroscopy in the Near-infrared with a Laser Frequency Comb

The discovery and characterization of exoplanets around nearby stars is driven by profound scientific questions about the uniqueness of Earth and our Solar System, and the conditions under which life could exist elsewhere in our Galaxy. Doppler spectroscopy, or the radial velocity (RV) technique, has been used extensively to identify hundreds of exoplanets, but with notable challenges in detecting terrestrial mass planets orbiting within habitable zones. We describe infrared RV spectroscopy at the 10 m Hobby-Eberly telescope that leverages a 30 GHz electro-optic laser frequency comb with nanophotonic supercontinuum to calibrate the Habitable Zone Planet Finder spectrograph. Demonstrated instrument precision <10 cm/s and stellar RVs approaching 1 m/s open the path to discovery and confirmation of habitable zone planets around M-dwarfs, the most ubiquitous type of stars in our Galaxy

Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex

Viral genetic tools to target specific brain cell types in humans and non-genetic model organisms will transform basic neuroscience and targeted gene therapy. Here we used comparative epigenetics to identify thousands of human neuronal subclass-specific putative enhancers to regulate viral tools, and 34% of these were conserved in mouse. We established an AAV platform to evaluate cellular specificity of functional enhancers by multiplexed fluorescent in situ hybridization (FISH) and single cell RNA sequencing. Initial testing in mouse neocortex yields a functional enhancer discovery success rate of over 30%. We identify enhancers with specificity for excitatory and inhibitory classes and subclasses including PVALB, LAMP5, and VIP/LAMP5 cells, some of which maintain specificity in vivo or ex vivo in monkey and human neocortex. Finally, functional enhancers can be proximal or distal to cellular marker genes, conserved or divergent across species, and could yield brain-wide specificity greater than the most selective marker genes

The consumer scam: an agency-theoretic approach

Despite the extensive body of literature that aims to explain the phenomenon of consumer scams, the structure of information in scam relationships remains relatively understudied. The purpose of this article is to develop an agency-theoretical approach to the study of information in perpetrator-victim interactions. Drawing a distinction between failures of observation and failures of judgement in the pre-contract phase, we introduce a typology and a set of propositions that explain the severity of adverse selection problems in three classes of scam relationships. Our analysis provides a novel, systematic explanation of the structure of information that facilitates scam victimisation, while also enabling critical scrutiny of a core assumption in agency theory regarding contract design. We highlight the role of scam perpetrators as agents who have access to private information and exercise considerable control over the terms and design of scam relationships. Focusing on the consumer scam context, we question a theoretical assumption, largely taken for granted in the agency literature, that contact design is necessarily in the purview of the uninformed principal

A sub-Neptune sized planet transiting the M2.5-dwarf G 9-40: Validation with the Habitable-zone Planet Finder

We validate the discovery of a 2 Earth radii sub-Neptune-size planet around the nearby high proper motion M2.5-dwarf G 9-40 (EPIC 212048748), using high-precision near-infrared (NIR) radial velocity (RV) observations with the Habitable-zone Planet Finder (HPF), precision diffuser-assisted ground-based photometry with a custom narrow-band photometric filter, and adaptive optics imaging. At a distance of $d=27.9\mathrm{pc}$, G 9-40b is the second closest transiting planet discovered by K2 to date. The planet's large transit depth ($\sim$3500ppm), combined with the proximity and brightness of the host star at NIR wavelengths (J=10, K=9.2) makes G 9-40b one of the most favorable sub-Neptune-sized planet orbiting an M-dwarf for transmission spectroscopy with JWST, ARIEL, and the upcoming Extremely Large Telescopes. The star is relatively inactive with a rotation period of $\sim$29 days determined from the K2 photometry. To estimate spectroscopic stellar parameters, we describe our implementation of an empirical spectral matching algorithm using the high-resolution NIR HPF spectra. Using this algorithm, we obtain an effective temperature of $T_{\mathrm{eff}}=3404\pm73$K, and metallicity of $\mathrm{[Fe/H]}=-0.08\pm0.13$. Our RVs, when coupled with the orbital parameters derived from the transit photometry, exclude planet masses above $11.7 M_\oplus$ with 99.7% confidence assuming a circular orbit. From its radius, we predict a mass of $M=5.0^{+3.8}_{-1.9} M_\oplus$ and an RV semi-amplitude of $K=4.1^{+3.1}_{-1.6}\mathrm{m\:s^{-1}}$, making its mass measurable with current RV facilities. We urge further RV follow-up observations to precisely measure its mass, to enable precise transmission spectroscopic measurements in the future.Comment: Accepted for publication in AJ, 22 pages, 15 figure