22 research outputs found

    Strategies for two-dimensional and three-dimensional field computation in the design of permanent magnet motors

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    This study discusses strategies for the design of permanent magnet motors (PMMs) exploiting two-dimensional (2D) and 3D field models. Five most common methodologies are compared and errors arising from 2D classical models considered. Examples comparing 2D and 3D results are presented and discussed for two selected types of motors. An approach has been put forward which allows the accuracy of classical 2D models to be improved by introducing correction coefficients arising from preliminary 3D simulations. A possibility of employing quasi-3D models has also been explored for the design and analysis of PMMs with the stator and rotor lamination packets of different lengths. Comparative analysis of results has been provided arising from the 2D and 3D models for a classical and a double rotor PMM

    Development of phoma lesions on oilseed rape leaves inoculated with ascospores of A-group or B-group Leptosphaeria maculans (stem canker) at different temperatures and wetness durations

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    In controlled-environment experiments, ascospores of both A-group and B-group Leptosphaeria maculans were able to infect leaves of oilseed rape and produce phoma leaf spot lesions at temperatures from 5 to 20 degreesC and wetness durations from 8 to 72 h after inoculation. Lesions formed on leaves inoculated with B-group ascospores had few pycnidia and were darker, smaller and less noticable than the larger, pale grey lesions with many pycnidia produced by A-group ascospores. Lesions formed by A-group or B-group L. maculans on naturally infected winter oilseed rape experimental crops were similar to lesions produced by the two groups on inoculated plants. The greatest numbers of lesions were produced with a leaf wetness duration of 48 h and at temperatures of 15-20 degreesC for both A-group and B-group ascospores. As leaf wetness duration and temperature decreased below the optimal values, the number of lesions decreased. The incubation period, estimated as the time from inoculation to the appearance of the first lesions (t(1)), or the time to the appearance of 50% of the lesions (t(50)), of B-group was often shorter than that of A-group L. maculans. As temperature decreased below 20 degreesC, the length of the incubation period of both A-group and B-group L. maculans increased.Peer reviewe

    Quantitative PCR analysis of abundance of airborne propagules of Leptosphaeria species in air samples from different regions of Poland

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    When airborne propagules of Leptosphaeria maculans and L. biglobosa were collected in Poland at three ecologically different sites from 1 September to 30 November in 2004 to 2008, using a Hirst-type seven-day volumetric spore trap, there were fluctuations in timing of ascospore release and diverse ratios between airborne propagules of both species depending on season, field location and weather conditions. The detection was done using the microscope as well as quantitative PCR with species-specific primers targeted against fragments of β-tubulin genes and quantified with a dual-labelled fluorescent probe approach. This detection chemistry is described for the first time for L. maculans and L. biglobosa. Its advantage over the previous ITS-based SYBR-Green chemistry resides in improved sensitivity and the virtual absence of false positives in the detection of these fungi. There were significant, positive correlations between data obtained using visual assessment of ascospore numbers and DNA concentrations that were measured by qPCR. Climatic differences between the oilseed rape growing regions could have significantly affected the biological processes of pseudothecial maturation and ascospore development of the pathogens. The data suggest that regular rain events of intermediate intensity recorded in the Maritime region favoured the maturation of the pathogen more than the drier weather recorded in the Silesia or Pomerania regions. It was observed that the number of rainy days was of greater importance than the cumulative rainfall to obtain the generative sporulation of the pathogen. Accurate detection of airborne inoculum of pathogenic Leptosphaeria spp. facilitates improved targeting of disease management decisions for oilseed rape protection against phoma stem canker and stem necrosis diseases.Peer reviewe
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