EVALUATION OF ANTIOXIDANT AND ANTICANCER ACTIVITIES OF CIPADESSA BACCIFERA

Objective: This study was conducted to evaluate the antioxidant and anticancer activities of methanolic extract of Cipadessa baccifera leaves.Methods: The total phenolic content in the extract was assessed by using Folin–Ciocalteu reagent. Antioxidant activity was assesses by differentmethods such as phosphomolybdate assay, ferric reducing power, 1,1-Diphenyl-2-picryl-hydrazyl, superoxide scavenging assay. Cytotoxic potentialof the extract against Ehrlich Ascites carcinoma (EAC), colon carcinoma (HT-29), and breast adenocarcinoma (MCF-7) cell lines was evaluated by3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay.Results: The total phenolic content present in the methanolic extract of C. baccifera was found to be 338.38 mg GAL/L. In phosphomolybdate assay,C. baccifera extract showed good antioxidant activity (half maximal inhibitory concentration [IC]=0.42 mg/ml) which was comparable to thestandard ferulic acid (IC50=0.22 mg/ml). The extract showed better cytotoxicity against EAC (IC50503=4.22 mg/ml) and HT-29 cell lines (IC=1.86 mg/ml)compared the MCF-7 cell lines (IC50=34.28 mg/ml).Conclusion: The extract showed good radical scavenging activity and anticancer activity against EAC and HT-29 cell lines. However, the activity wasless compared to the standard. Further analysis is required to confirm the in vivo activity of the extract. The extract showed negligible cytotoxic effectagainst MCF-7 cell lines.Keywords: Anticancer, Antioxidant, Methanolic extract, Cipadessa baccifera, Radical scavenging, 1,1-Diphenyl-2-picryl-hydrazyl assay, Ehrlich Ascitescarcinoma, HT-29, MCF-7

Antimicrobial, Wound Healing And Antioxidant Activities Of Anthocephalus cadamba

Anthocephalus cadamba (Roxb.) Miq. Syn A. chinensis (Lamk) A. Rich (Rubiaceae) is ethnomedicinally widely used in the form of paste by tribe in western Ghats for treating skin diseases. In this context, antimicrobial potential of A. cadamba against a wide range of microorganisms was studied. To validate the ethnotherapeutic claims of the plant in skin diseases, wound healing activity was studied, besides antioxidant activity to understand the mechanism of wound healing. The alchoholic and aqueous extract of this plant showed significant antibacterial and antifungal activity against almost all the organisms: Micrococcus luteus, Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, and four fungi Candida albicans, Trichophyton rubrum—dermatophyte fungi, Aspergillus niger, Aspergillus flavus and Aspergillus nidulans—systemic fungi, with especially good activity against the dermatophyte (Trichophyton rubrum) and some infectious bacteria (Escherichia coli, Proteus mirabilis and Staphylococcus aureus) with an MIC of 2.5 μg/disc. The results show that A. cadamba extract has potent wound healing capacity as shown from the wound contraction and increased tensile strength. The results also indicated that A. cadamba extract possesses potent antioxidant activity by inhibiting lipid peroxidation and increase in the superoxide dismutase (SOD) and catalase activity. Keywords: Anthocephalus cadamba; Antimicrobial activity; Antioxidant; Wound healing;African Journal of Traditional and Complementary Medicine Vol. 4 (4) 2007: pp. 481-48

Analgesic and Anti-Inflammatory Activities of Citrus Maxima (J.Burm) Merr in Animal Models

ABSTRACT The present study was aimed to investigate the analgesic and anti-inflammatory activities of Citrus maxima in animal models

Synthesis, antineoplastic and cytotoxic activities of some mononuclear Ru(II) complexes

A series of mononuclear Ru(II) complexes of the type [Ru(S)(2)(K)](2+), where S = 1,10-phenanthroline/2,2'-bipyridine and K = 4-OH-btsz, 4-CH(3)-btsz, 3,4-di-OCH(3)-btsz, 4-OH-binh, 4-CH(3)-binh, 3,4-di-OCH(3)-binh, were prepared and characterized by elemental analysis, FTIR, (1)H-NMR, and mass spectroscopy. The complexes displayed metal-ligand charge transfer (MLCT) transitions in the visible region. These ligands formed bidentate octahedral ruthenium complexes. The title complexes were evaluated for their in vivo anticancer activity against a transplantable murine tumor cell line, Ehrlisch's ascites carcinoma (EAC), and in vitro cytotoxic activity against human cancer cell lines Molt 4/C(8) and CEM and murine tumor cell line L1210. The ruthenium complexes showed promising biological activity especially in decreasing tumor volume and viable ascites cell counts. Treatment with these complexes prolonged the life span of mice bearing EAC tumors by 10-52%. In vitro evaluation of these ruthenium complexes revealed cytotoxic activity from 0.21 to 24 muM against Molt 4/C(8), 0.16 to 19 muM aginst CEM, and 0.75 to 32 muM against L1210.status: publishe

Estimation of bioflavonoids in Amaranthus caudatus Linn. by HPLC

Flavonoids are present in the Amaranthus caudatus Linn, Flavonoids like Rutin and quercetin possess many biochemical effects like inhibition of enzymes, regulatory role on different hormones and pharmacological activities like antimicrobial, antioxidant, anticancer, antihepatotoxic, protection of cardio vascular system. An HPLC method was developed for the estimation of rutin and quercetin from methanol herbal extract of Amaranthus caudatus

Anti-diarrhoeal activity of methanolic root extract of elephantopus scaber L

A study was undertaken to evaluate the effect of methanolic extract of the root of Elephantopus scaber L (MEES) against several experimental models of diarrhoea in rats. MEES showed significant inhibitory effect against castor-oil induced diarrhoea and PGE2 induced enteropooling in rats. The extract also showed a significant reduction in gastrointestinal motility in the charcoal meal test in rats. The results obtained establish the efficacy and substantiate the folkloric claim of the plant as an anti-diarrhoeal agent

Antimicrobial effects of Indian medicinal plants against acne-inducing bacteria

Propionibacterium acnes and Staphylococcus epidermidis have been recognized as pus-forming bacteria triggering an inflammation in acne. The present study was conducted to evaluate antimicrobial activities of Indian medicinal plants against these etiologic agents of acne vulgaris. Ethanolic extracts of Hemidesmus indicus (roots), Eclipta alba(fruits), Coscinium fenestratum (stems), Curcubito pepo (seeds), Tephrosia purpurea (roots), Mentha piperita (leaves), Pongamia pinnata (seeds), Tinospora cordyfolia (barks), Euphorbia hirta (roots), Tinospora cordyfolia (roots), Thespesia populnea(roots), and Jasminum officinale (flowers) were tested for antimicrobial activities by disc diffusion and broth dilution methods. The results from the disc diffusion method showed that 07 medicinal plants could inhibit the growth of Propionibacterium acnes. Among those Hemidesmus indicus, Coscinium fenestratum , Tephrosia purpurea , Euphorbia hirta, Tinospora cordyfolia , Curcubito pepo and Eclipta albahad strong inhibitory effects. Based on a broth dilution method, the Coscinium fenestratum extract had the greatest antimicrobial effect. The MIC values were the same (0.049 mg/ml) for both bacterial species and the MBC values were 0.049 and 0.165 mg/ml against Propionibacterium acnes and Staphylococcus epidermidis, respectively. In bioautography assay, the Coscinium fenestratum extract produced strong inhibition zones against Propionibacterium acnes. Phytochemical screening of Coscinium fenestratum revealed the presence of alkaloid which could be responsible for activity. Taken together, our data indicated that Coscinium fenestratum had a strong inhibitory effect on Propionibacterium acnes and Staphylococcus epidermidis