Estimation of the subunit stoichiometry of the membrane-associated daptomycin oligomer by FRET

The final publication is available at Elsevier via http://doi.org/10.1016/j.bbamem.2012.02.019 © 2012. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/Daptomycin is a lipopeptide antibiotic that kills Gram-positive bacteria by depolarizing their cell membranes. This antibacterial action of daptomycin is correlated with the formation of membrane-associated oligomers. We here examine the number of subunits contained in one oligomer using fluorescence resonance energy transfer (FRET). The results suggest that the oligomer contains approximately 6 to 7 subunits, or possibly twice this number if it spans both membrane monolayers.This work was supported by an NSERC operating grant to M. Palmer. J. Muraih was supported by a scholarship from the government of Iraq

Sensitive Cloud Point Extraction Methodology for Separation Preconcentration of Co(II) Followed by Spectrophotometric Determination in Different Samples

With application CPE methodology to separation preconcentration and extraction Co2+ion used 2-[4-antybyren zolylazo]-1,2-dihydroxy-9,10-anthracene-dione (AADAD) as complexing agent to form ion pair complex as well as used non-ionic surfactant 1% Tritonx-100 at PH=8 , also this research involved limitation optimum conditions as well as thermodynamic study , Synergism effect and spectrophotometric determination of Co(II) in different samples with detection limit(D.L) =(1.6559×10-5µg.mL-1) and Sandell’s sensitivity =(7.99×10-5mg.cm-2) and ε =(7374.12L.mol-1.cm-1 )and RSD% =(%0.0065). Keywords:Cobalt(II) , Cloud point layer ,TritonX-100

On best one-sided approximation by multivariate Lagrange Interpolation in weighted spaces

Degree of best one-sided approximation of multivariate function  that lies in weighted space ( -space) by construct an operator which is dependent on multivariate Lagrange Interpolation  that satisfies....

Fractal Geometry: An Attractive Choice for Miniaturized Planar Microwave Filter Design

Various fractal geometries are characterized by the self-similarity and space-filling properties. The space-filling feature has been successfully applied to design multiband antenna structures for a wide variety of multifunction wireless systems. On another hand, the second feature has proved its validity to produce miniaturized antennas and passive microwave circuits including the band-pass filters (BPF). This chapter demonstrates the design of miniaturized microstrip BPFs that are derived from fractal-based DGS resonators. Many microstrip BPFs based on the Minkowski fractal DGS resonators will be presented together with those based on Moore and Peano fractal geometries. Simulation results, of all of the presented BPFs, show that an extra-size reduction can be obtained as the iteration level becomes higher. Measured and simulated results agree well with each other. A comparison has been conducted with other filters based on Peano and Hilbert fractal geometries. The results reveal that the proposed BPF offers acceptable performance and a significant decrease of higher harmonics

SENSITIVE SOLVATION METHOD FOR SEPARATION SO DETERMINATION OF ZINC(II) AND CADMIUM(II) IN DIFFERENT SAMPLES.

ABSTRACTObjective: Separation and extraction of Zn(II) and Cd(II) as a neutral compound by used methyl stearate as extractant dissolved in chloroform.Methods: Applications of separation and extraction according to solvation method from nitrate aqueous solution by used methyl stearate which isbinding coordinally with nitrate salt of metal under study Zn(II) and Cd(II).Results: The extracted species have λmax=263 nm for Zn2+ and λmax=346 nm for Cd. The studies about limitation optimum condition for extractionshow 0.3 M salting out effect KNO3 for Zn2+ and 0.7 M KNO3 for Cd2+2+ in present 100 µg Zn2+/5 ml and 50 µg Cd/5 ml with optimum shaking time15 minutes for both ions.2+Conclusion: The separation by solvation method shows high sensitivity and selectivity in addition to high extraction efficiency for separation Zn(II)and Cd(II). As well illustrate the important rule of kind and concentration of salting out agent and different extractions efficiency with differ extractant.Key words: Solvation method, Separation and determination, Zinc(II), Cadmium

Cloud Point Extraction Methodology for Separation and Extraction Platinum (II) as Chloro Complex Anion Coupled with Spectrophotometric Method for Determination in Different Samples

Whereas the platinum is very important metal with several special properties to provide it as expensive metal with important using , for separation preconcentration and extraction platinum as Chloroanion PtCl4= according to cloud point extraction method used Triton X-100 at optimum conditions such as 1M HCl with 1x10-4M Janus green (JG) and 0.5 mL of 1%  Triton X-100 Cloud point layer Formation at 75?C of heating in electrostatic water bath for 15 minutes , The stoichiometric study show the more probable structure of ion association Complex extraction was [JG+ ; HPtCl4-] , with thermodynamic data ?Hex = 0.0814KJ mol-1 , ?Gex =-61.42 KJ mol-1 and ?Sex = 176.7J mol-1 K-1, the main method for extraction show D.L = 6.8028*10-6 S=0.003133 µg cm-1 R.S.D= 0.00885                               the maximum absorbance wave length was = 690 nm  and ?= 6227.861 L mol-1 cm-1 Keywords: platinum, cloud point extractio

A high security and noise immunity of speech based on double chaotic masking

It is known that increasing the security of the information and reducing the noise effect through public channels are two of the main priorities in developing any communication system. In this article, an efficient, secure communication system with two levels of encryption has been applied to the speech signal. The suggested security approach was implemented by using two different stages of chaotic masking on the signal; one masking was conducted by using Lorenz system and the other masking was built by using Rӧssler chaotic flow system. The main goal of developing this two-chaotic masking approach is to increase the key space and the security of the information. Also, an immunity technique has been implemented in the suggested approach to reduce the noise effect. For practical application purposes, this system was tested with additive white gaussian noise (AWGN) channel. The simulation results show that the quality of reconstructed speech signal is changeable according to the used signal to noise ratio (SNR); therefore, a proposed technique based on digital processing method (DPM) was applied to the first masked signal by converting the sampled data from the analog to the binary format. The simulation results show that an 22 dB (SNR) is sufficient to recover the speech signal with minimum noise by using the suggested approach

Maximizing Resource Effectiveness of Highway Infrastructure Maintenance Inspection and Scheduling for Efficient City Logistics Operations

AbstractThe safe and efficient movement of people and cargo on roadways is dependent on a well functioning highway system which requires effective maintenance policies focused on the maximum use of invested resources. For efficient city logistics operations various components of highway infrastructure, such as pavements, guardrails, and roadside signs must be maintained and kept in acceptable operating condition as elements of an integrated highway network. Due to the shrinking budget for undertaking infrastructure inspection and maintenance activities Resource Effectiveness (RE) becomes paramount. Theoretically, Resource Effectiveness (RE) is intended to get the optimum performance out of a project or investment. Instead of adding more resources (like labor, equipment, etc.), it will concentrate on the prudent utilization of resources or investment. Thus, RE in a broader sense, is concerned with the prudent use of labor, equipment, and material. In this paper we develop an optimization approach for maximizing resource effectiveness of highway infrastructure maintenance investments, subject to budget constraints, based on the concept of the well-known Cobb-Douglas production function. Several examples are presented

An Analytic Solution for Riccati Matrix Delay Differential Equation using Coupled Homotopy-Adomian Approach

في هذا البحث تناولنا طريقة فعالة وجديدة وهي الدمج بين طريقتي الهوموتوبي و الادوميان مع إستخدام مفهوم المعادلات التفاضلية الاعتيادية التباطئية لحل معادلة المصفوفات التباطئية لمعادلة ريكاتي والحصول على حل تقريبي دقيق جدا قليل الخطأ ويقترب من الحل المظبوط او الحل الحقيقي. في هذه الطريقة تم الكشف على نتائج ادق خلال فترة التأخير التي مرت بها المعادلة. أيضا في هذه الطريقة ان الاقتراب للحل الحقيقي يأخذ منطقة او فترة اوسع وكلما استمرينا ب التكرارا حيث نحصل على نتائج دقيقة جدا ويكون الخطأ جدا صغير.An efficient modification and a novel technique combining the homotopy concept with  Adomian decomposition method (ADM) to obtain an accurate analytical solution for Riccati matrix delay differential equation (RMDDE) is introduced  in this paper  . Both methods are very efficient and effective. The whole integral part of ADM is used instead of the integral part of homotopy technique. The major feature in current technique gives us a large convergence region of iterative approximate solutions .The results acquired by this technique give better approximations for a larger region as well as previously. Finally, the results conducted via suggesting an efficient and easy technique, and may be addressed to other non-linear problems