The impact of multi-location work on reaching carbon neutrality objectives:the carbon footprint of ICT equipment use at the University of Oulu

Abstract. This thesis project aimed to quantify the impact of multi-location work on the carbon footprint of the University of Oulu by conducting a survey and monitoring the electricity consumption of ICT equipment. The work presents the concept of carbon neutrality, the Greenhouse Gas Protocol quantifying carbon emissions. In addition, global decarbonization commitments and the pledges of higher education sector are introduced. Furthermore, a benchmarking of decarbonization actions of higher education institutes was done, reviewing 29 case studies. The scopes of emissions considered in their carbon footprint were reviewed, as well as their chosen mitigation methods. The effect of multi-location work on Higher Education Institutions and especially its impacts on the use of Information and Communication Technologies (ICT) are presented. The carbon footprint of ICT equipment, the internet, and their calculation methods are also illustrated. In addition, a survey was conducted among the staff of the University of Oulu, to collect data about the various types of ICT equipment that staff use on-campus, in their home office, and those being carried in-between these two workplaces. The survey also uncovered information about the staff’s ICT equipment usage habits. In addition, electricity consumption monitoring of laptops and monitors was done to increase the accuracy of the carbon footprint calculation. The results achieved from electricity consumption monitoring of ICT equipment demonstrate that the electricity consumption of laptops during meeting hours is approximately 2.5 times higher than the electricity consumption during regular work. Further, when leaving the laptop on and not using it, it could consume almost as much as using it on a regular basis. On the other hand, putting the laptop in sleep mode consumes only 17% electricity compared with when it is left on and not used. Furthermore, it was also found out that the laptop would consume up to 65% more when the VPN is on. The staff survey also revealed that, on average, staff would prefer working three days from home per week. This would indicate a 36% increase of the carbon footprint of the ICT equipment. It is expected that the results of this work will help raising awareness and change habits regarding ICT equipment usage, in order to contribute to the carbon mitigation goals of the University of Oulu

Phylogeography of cliff racer (Platyceps rhodorachis Jan, 1865) from Punjab, Pakistan

The present study reports the existence of cliff racer, Platyceps rhodorachis from the plains of Punjab, Pakistan. A total of 10 specimens were captured during the field surveys from June to September, 2018 from different sites of Punjab. Platyceps rhodorachis was identify on the basis of morphology and confirmed through COI gene sequences. The obtained DNA sequences have shown reliable and exact species identification. Newly produced DNA sequences of Platyceps rhodorachis were submitted to GenBank and accession numbers were obtained (MK936174.1, MK941839.1 and MT790210.1). N-J tree based on COI sequences of Platyceps rhodorachis clearly separated as out-group with other members of family Colubridae based on p-distance. The intra-specific genetic variation ranges from 12% to 18%. The DNA sequences of Platyceps rhodorachis kashmirensis, Platyceps rhodorachis ladacensis, Platyceps ventromaculatus, Platyceps ventromaculatus bengalensis and Platyceps ventromaculatus indusai are not available at NCBI to validate their taxonomic positions. In our recommendations, a large scale molecular based identification of Pakistan’s herpetofauna is required to report more new or subspecies from country.S. Malika, A. Javida, Hamidullahb, M. A. Iqbalc, A. Hussaina, S. M. Bukharia, A. L. Chaberd, F. Hemmatzadehd, N. Ismate, A. Alif, S. M. Azamg, G. Mustafaa, S. Hussaina, U. Ahmada, A. Ahmadh, and W. Ali

Frequent pUL27 Variations in HIV-Infected Patients

Objective: Drug-resistant isolates of human cytomegalovirus (HCMV) have led to the development of new anti-HCMV drugs. Maribavir (MBV) is a novel inhibitor of the HCMV viral kinase. Resistance to MBV is mapped to gene UL27, a viral nuclear protein. In this study, we investigated UL27 polymorphisms in MBV-naive HIV-positive and HCMV congenitally infected clinical samples. Methods: DNA was extracted from 20 CMV-positive HIV (9/20) and congenitally infected (11/20) patients and used for UL27 polymerase chain reaction amplification. Sanger sequencing and multiple sequence alignment of products was performed. Results: K90 was the most prevalent polymorphism in both HIV-positive and congenitally infected patients. Polymorphisms Q54, D123, and R107 (10) were seen in more than one sample. There were significantly more polymorphisms in the HIV-positive samples (p = 0.038). Conclusion: HCMV pUL27 is highly variable in adult immunocompromised HIV-positive patients. © 2017 S. Karger AG, Base

Human cytomegalovirus pUL97 drug-resistance mutations in congenitally neonates and HIV-infected, no-drug-treated patients

Aim: Human cytomegalovirus (HCMV) treatment is hard to achieve because of viral protein target sequence variations. Objectives: We aimed to find HCMV pUL97 kinase variations in HIV- and congenitally infected patients. Methods: Twenty HCMV-positive DNA samples from nonganciclovir treated congenitally infected neonates and HIV positive patients were used for PCR restriction fragment length polymorphism. Variations were assessed computationally for pUL97 functionality. Results: P521L, D605E and N597Y substitutions were prevalent significantly in congenital infection. Furthermore, we found those mutations have neutral or low impact on pUL97 functionality. In addition, we found a new K599Q substitution in an HIV-infected individual. Conclusion: More prevalent substitutions related to low-grade ganciclovir resistance were found in congenitally infected neonates in comparison with HIV-infected patients. © 2017 Future Medicine Ltd

Comparison of p.o. or i.v. proton pump inhibitors on 72-h intragastric pH in bleeding peptic ulcer

BACKGROUND AND AIMS: After successful endoscopic hemostasis in bleeding peptic ulcer, addition of proton pump inhibitors reduce the rate of recurrent bleeding by maintaining intragastric pH at neutral level. The aim of the present study was to evaluate the effect of various proton pump inhibitors given through different routes on intragastric pH over 72 h after endoscopic hemostasis in bleeding peptic ulcer

Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and Azerbaijan

The objective of this study was to identify gliadin band patterns and the extent of genetic diversity in durum wheat genotypes from Northwestern Iran and the Republic of Azerbaijan. Gliadins from 46 landraces and four cultivars were evaluated through acid PAGE analyses. Sixty-six polymorphic bands and 81 patterns were identified. Twenty-four different motility bands and 22 patterns were found in the ω gliadin region with 14 polymorph bands and 20 patterns for α and γ gliadins, and 14 bands and 19 different patterns for β gliadins. The combination of these patterns generated 38 and 39 combinations for Gli-1 and Gli-2 loci, respectively. The genetic diversity index (H) was higher for α gliadins (0.924), followed by ω and γ gliadins (0.899 and 0.878, respectively), and for β gliadin patterns (0.866). Extensive polymorphism (H = 0.875) was observed in four gliadin pattern regions, with higher genetic diversity in the Iranian landraces than in the Azerbaijani ones. Each genotype had special identifying patterns in the gliadin acid PAGE analysis, and cluster analysis based on Jaccard's similarity coefficients formed six groups. Gliadin has a simple, repeatable and economic analysis, and can be used in genetic studie