Tendon Is Covered by a Basement Membrane Epithelium That Is Required for Cell Retention and the Prevention of Adhesion Formation

The ability of tendons to glide smoothly during muscle contraction is impaired after injury by fibrous adhesions that form between the damaged tendon surface and surrounding tissues. To understand how adhesions form we incubated excised tendons in fibrin gels (to mimic the homeostatic environment at the injury site) and assessed cell migration. We noticed cells exiting the tendon from only the cut ends. Furthermore, treatment of the tendon with trypsin resulted in cell extravagation from the shaft of the tendons. Electron microscopy and immunolocalisation studies showed that the tendons are covered by a novel cell layer in which a collagen type IV/laminin basement membrane (BM) overlies a keratinised epithelium. PCR and western blot analyses confirmed the expression of laminin β1 in surface cells, only. To evaluate the cell retentive properties of the BM in vivo we examined the tendons of the Col4a1+/Svc mouse that is heterozygous for a G-to-A transition in the Col4a1 gene that produces a G1064D substitution in the α1(IV) chain of collagen IV. The flexor tendons had a discontinuous BM, developed fibrous adhesions with overlying tissues, and were acellular at sites of adhesion formation. In further experiments, tenotomy of wild-type mice resulted in expression of laminin throughout the adhesion. In conclusion, we show the existence of a novel tendon BM-epithelium that is required to prevent adhesion formation. The Col4a1+/Svc mouse is an effective animal model for studying adhesion formation because of the presence of a structurally-defective collagen type IV-containing BM

Modeling sequential context effects in judgment analysis

In this article a broad perspective incorporating elements of time series theory is presented for conceptualizing the data obtained in multi-trial judgment experiments. Recent evidence suggests that sequential context effects, assimilation and contrast, commonly found in psychophysical judgment tasks, may be present in judgments of abstract magnitudes. A time series approach for analyzing single-subject data is developed and applied to expert prognostic judgments of risk for heart disease with an emphasis on detecting possible sequential context effects. The results demonstrate that sequential context effects do exist in such expert prognostic judgments. Contrast and assimilation were produced by cue series; the latter occurring more frequently. Experts also showed assimilation of prior responses that was independent of the cue series input. Time series analysis also revealed that abrupt or large trial-by-trial changes in the value of cues that receive the most attention in prognostic judgment tasks can disrupt the accuracy of these judgments. These findings suggest that a time series approach is a useful alternative to ordinary least squares regression, providing additional insights into the cognitive processes operating during multi-cue judgment experiments

Modeling sequential context effects in judgment analysis: A time series approach

In this article a broad perspective incorporating elements of time series theory is presented for conceptualizing the data obtained in multi-trial judgment experiments. Recent evidence suggests that sequential context effects, assimilation and contrast, commonly found in psychophysical judgment tasks, may be present in judgments of abstract magnitudes. A time series approach for analyzing single-subject data is developed and applied to expert prognostic judgments of risk for heart disease with an emphasis on detecting possible sequential context effects. The results demonstrate that sequential context effects do exist in such expert prognostic judgments. Contrast and assimilation were produced by cue series; the latter occurring more frequently. Experts also showed assimilation of prior responses that was independent of the cue series input. Time series analysis also revealed that abrupt or large trial-by-trial changes in the value of cues that receive the most attention in prognostic judgment tasks can disrupt the accuracy of these judgments. These findings suggest that a time series approach is a useful alternative to ordinary least squares regression, providing additional insights into the cognitive processes operating during multi-cue judgment experiments.expert judgment, time series, contrast and assimilation, single-subject analysis.

A note on determining the number of cues used in judgment analysis studies: The issue of type II error

Many judgment analysis studies employ multiple regression procedures to estimate the importance of cues. Some studies test the significance of regression coefficients in order to decide whether or not specific cues are attended to by the judge or decision maker. This practice is dubious because it ignores type II error. The purposes of this note are (1) to draw attention to this issue, specifically as it appears in studies of self-insight, (2) to illustrate the problem with examples from the judgment literature, and (3) to provide a simple method for calculating post-hoc power in regression analyses in order to facilitate the reporting of type II errors when regression models are used.judgment analysis, self-insight, multiple regression, post-hoc power.

Assessing Cross-Cultural Validity of Scales: A Methodological Review and Illustrative Example

In this article, we assessed the cross-cultural validity of the Women's Role Strain Inventory (WRSI), a multi-item instrument that assesses the degree of strain experienced by women who juggle the roles of working professional, student , wife and mother. Cross-cultural validity is evinced by demonstrating the measurement invariance of the WRSI. Measurement invariance is the extent to which items of multi -item scales function in the same way across different samples of respondents. We assessed measurement invariance by comparing a sample of working women in Taiwan with a similar sample from the United States. Structural equation models (SEMs) were employed to determine the invariance of the WRSI and to estimate the unique validity variance of its items. This article also provides nurse-researchers with the necessary underlying measurement theory and illustrates how SEMs may be applied to assess cross-cultural validity of instruments used in nursing research. Overall performance of the WRSI was acceptable but our analysis showed that some items did not display invariance properties across samples. Item analysis is presented and recommendations for improving the instrument are discussed. (C) 2006 Elsevier Ltd. All rights reserved

Motivation and the Senior Athlete: An Examination of the Psychometric Properties of the Sport Motivation Scale

The Sport Motivation Scale (SMS) is commonly used to measure motivation in young athletes. The purpose of this investigation was to examine the factorial validity of the SMS among a sample of senior athletes. A correlational research design, in addition to confirmatory factor analyses (CFAs), was used to examine the factor structure of the SMS in a sample of senior game participants at the Florida Senior State Championships held in Kissimmee, Fla, from November 28 to December 3, 2000. Results of psychometric testing on the SMS did not support the hypothesized factor structure of this instrument in assessing motivation in the senior athlete population