Comprehensive microsatellite allele table for 11 loci

Microsatellite alleles used in study for each sampled site. Some individuals were less successful and may have been removed before final analysis. Location information can be found in the main paper (GPS, etc.)

Nuclear_520_sequence_alignment

Aligned nuclear marker 520 sequences for all locations

usat_tree_rey

Reynolds distance microsatellite tre

Nuclear_508_sequence_alignment

Aligned nuclear marker 508 sequences for all location

Species Tree

Species tree in Newick format

usat_tree_nei

Nei;s distance microsatellite tre

Dicamptodon copei microsatellite genotype data

Dicamptodon copei tissue samples were collected from the field in 2006-2008. DNA was extracted using DNeasy tissue kits (Qiagen) according to the manufacturer’s protocols. Eleven microsatellite loci were amplified using PCR with fluorescently labeled primers, using PCR conditions from Steele et al. (2008) (Table S1, Supporting Information). Microsatellite products were run on an ABI 3730XL automated sequencer (Applied Biosystems) at the Washington State University LBB1 core facility and genotyped using ABI GENEMAPPER 3.7 software. The data include microsatellite fragment lengths for each study site (rows) and (diploid) locus (columns)

Dcopei_landscape_distances

These data are landscape distances between study sites calculated using least cost path (LCP) analysis (columns B-JJ) and Circuitscape resistance analysis (columns JL-KB). LCP data represent the weighted averages of all landscape layers along a particular LCP (indicated in the row below the region name), and all LCPs were corrected for topography. Landscape cost surfaces were developed using the following GIS datasets downloaded from online databases (including abbreviations and source of data citations from Trumbo et al. 2012): stream networks (USGS 2001) (str10 = 10:1 cost of movement outside streams, str100 = 100:1 cost of movement outside streams), canopy cover (cc) (Homer et al. 2004), frost-free periods (ffp) (Rehfeldt 2006), growing season precipitation (gsp) (Rehfeldt 2006), landcover (lc) (Homer et al. 2004), and heat load index (hli) (McCune and Keon 2002). For streams, a "no" following abbreviation means that large rivers were excluded from the stream network. For all continuous variables, a "1" following the abbreviation means an exponentially increasing non-linear response, such that the transformed surface T1 = 100^ (original cost surface). A "2" following the abbreviation means an exponentially decreasing non-linear response such that the transformed surface T2 = 100 - 100^(1- original cost surface). The "strghtline" data are null models of isolation by Euclidean distance alone

Cytb_sequence_alignments

Aligned Mitochondrial cytb sequences for each locatio

usat_genotypes

Microsatellite genotypes in genepop format