Trichomonas tenax u usnoj šupljini

Fifty-one patients were investigated in order to determine the presence of Trichomonas tenax in various oral structures (saliva, dental plaque from physiologically clean and unclean tooth areas, carious dentin, root canal contents). Samples were cultivated in liquid Diamond’s axenic broth medium. The results were considered positive if living oral Trichomonas was found in smears. The prevalence of Trichomonas tenax in the study patients was 35.5%. Trichomonas tenax was most frequently found in dental plaque (23.2%) and in the root canal content (30.0%) (p > 0.05). The variables observed in this study (patient’s age and sex, oral hygiene, gingivitis, number of carious teeth, smoking and coffee intake) did not influence the presence of Trichomonas tenax. According to the results obtained, a mature dental plaque appears to be the oral structure favoring the growth and survival of oral Trichomonas in the physiologic conditions. It is possible that Trichomonas tenax spreads from dental plaque into the diseased endodontium.Prisutnost Trichomonas tenaxa na različitim strukturama usne šupljine (slina, dentobakterijski plak s fiziološki čistoga i nečistoga mjesta na zubu, karijesni dentin, sadržaj korijenskih kanala) istražena je u 51 ispitanika. Uzorci su kultivirani na Diamondovoj akseničnoj hranjivoj tekućoj podlozi. Nalaz živoga parazita u uzorku smatran je pozitivnim nalazom. Prevalencija Trichomonas tenaxa u ispitanika iznosila je 35,3%, a nađen je u zubnom plaku (23,2%) i u korijenskim kanalima (30,0%) (p > 0,05). U našem istraživanju ispitivane varijable (dob i spol ispitanika, stupanj oralne higijene, upala gingive, broj karijesnih zubi, pušenje i konzumiranje kave) nisu utjecale na pojavnost Trichomonas tenaxa. Prema rezultatima ovoga rada, zreli zubni plak omogućuje rast i razvoj oralnoga Trichomonasa u fiziološkim uvjetima u usnoj šupljini, a Trichomonas može prijeći iz zubnoga plaka u bolesni endodont

Subcutaneous dirofilariosis — a case report

Humana dirofilarioza je u Hrvatskoj rijetka. Prikazali smo dva slučaja, koja su se manifestirala bezbolnim supkutanim čvorićima u trajanju od nekoliko mjeseci. Prvi bolesnik je 44-godišnja kućanica iz ruralne sredine. Drugi je bolesnik 66-godišnji muškarac iz Zagreba, koji nema životinja i nije izbivao izvan Hrvatske. U oba slučaja su potkožni čvorići kirurški ekscidirani i histološki obrađeni nakon čega su bolesnici bili izliječeni. Ističemo značaj dirofilarioze u diferencijalnoj dijagnozi potkožnih čvorića.Human dirofilariosis is very rare in Croatia. We present two cases that manifested in painless subcutaneous nodules lasting for several months. The first patient was a 44-year-old housewife from rural environment. The second patient was a 66-year-old male from Zagreb that kept no domestic animals, and did not reside outside Croatia. In both cases subcutaneous nodules were surgically removed and histological analysis was performed, after which both patients were cured. The authors stress the importance of dirofilariosis in differential diagnostics of subcutaneous nodules

Abdominal ultrasound diagnostics of infectious diseases

Abdominalni ultrazvuk rabi transmisiju i refleksiju ultrazvučnih valova za prikaz unutarnjih organa kroz trbušnu stijenku. Opisali smo klinički značaj i sonografske karakteristike različitih oblika žarišnih i difuznih jetrenih lezija te upalnih stanja žučnog sustava, gušteraće i slezene kao i diferencijalno dijagnostičke mogućnosti. Izuzevši jednostavne ciste i tipične hemangiome, definitivna karakterizacija jetrenih lezija nije uvijek moguća konvencionalnim ultrazvukom. Ovaj pregledni rad također opisuje karakteristike transabdominalnog ultrazvuka tankog i debelog crijeva kod različitih infektivnih bolesti i upalnih stanja.Abdominal ultrasonography uses the transmission and reflection of ultrasound waves to visualise internal organs through the abdominal wall. We describe the clinical significance and sonographic features of various types of liver focal and diffuse infections, biliary, pancreatic and splenic inflammatory conditions and differential diagnosis. With the exception of cysts and typical haemangiomas, a definitive characterisation of liver lesions is often not possible using conventional ultrasound. This review also provides an overview of transabdominal ultrasonography of the small and large bowel, while summarizing its use in a variety of gastrointestinal infectious and inflammatory diseases

Proteolytic Activity of Trichomonas tenax from Dentobacterial Plaque and Root Canal Contens

Ispitivala se je proteolitička aktivnost oralnoga protozoona Trichomonas tenax kultiviranog izravno iz zubnoga plaka i sadržaja korijenskih kanala zuba s upalno promijenjenom ili gangrenoznom pulpom, ili periapeksnim procesom. Nakon pripreme lizata, utvrđivala se je proteolitička aktivnost oralnoga trihomonasa elektroforezom na poliakrilamidnom gelu i elektroforezom na poliakrilamidnom gelu s dodatkom želatine. Najbrža i najizrazitija aktivnost obiju vrsta izolata oralnoga trihomonasa opažena je pri inkubiranju gela u pH 4,6; zatim u pH 5,6. Aktivnost je bila još očita kod pH 2,8, a kod pH 7,2 izrazito slaba. Proteolitička aktivnost bila je najizraženija u području proteina molekulske težine 36 kDa. Rezultati ovisni o pH inkubirajučih medija pokazuju pstojanje različitih endopeptidaza u lizatima stanica protozoona Trichomonas tenax iz zubnoga plaka i korijenskih kanala.The purpose of this study was to examine proteolytic activity of Trichomonas tenax collected directly from dentobacterial plaque and root canal contents o f gangrenous teeth or teeth with periapical processes. Both electrophoretic method involving polyacrylamide gels and electrophoretic method involving gelatin - containing polyacrylamide gels, have been used to analyse Trichomonas tenax proteolytic activity. The most obvious and fastest activities were obtained when gels were incubated in pH 4.6, followed by the results of incubating in pH 5.6, while in pH 2.8 activity was less but still obvious. Proteolytic activities were the most effective in the area of protein MW 36 kDa. The different activity of enzymes depending on the pH o f incubated media indicate the presence of different endopeptidases in cell lysates of protozoon Trichomonas tenax from dentobacterial plaque and root canal contents

Peryferie w języku, literaturze i kulturze chorwackiej

Księga abstraktów oraz program międzynarodowej konferencji kroatystycznej, zorganizowanej w związku z jubileuszem 25-lecia filologii chorwackiej na Uniwersytecie Śląskim w Katowicach