Detection of Gene Involved in Biofilm Formation in Methecillin Resistance Staphylococcus aureus and Staphylococcus lentus and Effect of Camphor extract on These Genes

This research aimed to investigate the antibacterial activity of Camphor extract ,against biofilm formation of clinical isolates Staphylococcus aureus and Staphylococcus lentus .by detecting the presence and absence of virulence genes that responsible for the biofilm formation after treatment  with Camphor  extract. .thirty isolates were diagnosed initially as Staphylococci then selected five isolates depending on resistance to different type of antibiotics as virulence .After that the isolates test by VITEK-2compact system (ID and AST) to confirm the species of Staphylococci .The diagnostic result showed that three isolates belong to Staphylococcus aureus and two to Staphylococcus lentus. Investigation of the ability of camphor oil to inhibit the growth of bacteria using diffusion method in Muller Hinton agar ,the results showed the effect of camphor oil to prevent biofilm formation  on both bacterial species with concentration (0.1 g/ml )

A study of bacterial types associated with males infertility in AL-Najaf AL-Ashraf Governorate.

A total of 328 seminal fluid samples were collected from infertile patients with various types of infertility; Azoospermia, oligozoospermia, asthenozoospermia, teratozoospermia aged between 19 to 60 years, in comparsion with control group (Normospermia) during march until August 2013.      The biggest number of infertility were in age 19-29 years which reached 143(44%) ,followed by 30-39 years 109 (33%) and ˃ 40 years  76(23%) . There are  significant differences between various infertility types and age groups (p˂0.05).                                                                    A total of 48 isolations of Gram positive and negative bacterial Types were isolated  Staphylococcus aureus has a highly prevalence from (45) infertile patients numbered 15 isolates in percentage (29%) , followed by other bacterial types : Streptococcus pyogenes ,  Klebsiella  Spp. , Escherichia coli, Enterobacter Spp.  and  Neisseria gonorrhea that numbers of isolations and percent ages as follows: 14(29%),7(15%) ,3 (6%),and 2(4%)  respectively . statistically, there are significant differences between numbers of bacterial isolates (P˂0.05).