PENGARUH EKSTRAK BUAH DELIMA MERAH TERHADAP KADAR SOD PADA KULTUR HUVECs YANG DIPAPAR PLASMA PREEKLAMPSI

Preeklampsi merupakan sindroma yang berkaitan dengan adanya gejala vasospasme, peningkatan terhadap resistensi pembuluh darah perifer dan juga penurunan perfusi organ. Salah satu teori etiologi preeklampsi yang dianut saat ini mengatakan adanya ketidakseimbangan antara produksi radikal bebas dan sistem pertahanan antioksidan yang menyebabkan timbulnya stres oksidatif, ditandai dengan adanya penurunan aktivitas antioksidan disertai dengan adanya peningkatan kadar lipid peroksida (oksidan / radikal bebas). Keadaan ini tentu saja menimbulkan terganggunya fungsi endotel bahkan rusaknya seluruh struktur sel endotel yang disebut sebagai disfungsi endotel. Keadaan stres oksidatif bisa dikendalikan dengan pemberian anti oksidan yang terbagi dalam antioksidan enzimatis dan non enzimatis. Antioksidan enzimatis terdiri dari superoksida dismutase (SOD), glutation peroksidase dan katalase. Buah delima mengandung fitokimia dan kaya akan senyawa yang berfungsi sebagai antioksidan yaitu polifenol, tanin dan anthocyanin. Tujuan penelitian ini adalah untuk mengetahui efek pemberian ekstrak buah delima merah merah (Punica granatum) terhadap kadar SOD dan MDA pada kultur HUVECs yang dipapar plasma preeklampsi. Rancangan percobaan berupa rancangan acak lengkap dengan 5 kelompok perlakuan yaitu, Kelompok I : Kontrol negatif (K-) HUVECs + plasma kehamilan normal 2%, Kelompok II : Kontrol positif (K+) HUVECs + plasma preeklampsi 2%, Kelompok III : Perlakuan 1 (P1) HUVECs + plasma preeklampsi 2% + ekstrak buah delima merah 14 ppm, kelompok IV : Perlakuan 2 (P2) HUVECs + plasma preeklampsi 2% + ekstrak buah delima merah 28 ppm dan Kelompok V : Perlakuan 3 (P3) HUVECs + plasma preeklampsi 2% + ekstrak buah delima merah 56 ppm. Berdasarkan pada hasil uji LSD 5 % menunjukkan bahwa kelompok kontrol negatif (K-) memiliki rata-rata kadar SOD berbeda nyata dengan kelompok kontrol positif (K+) (p = < 0,05). Peningkatan kadar SOD secara signifikan ditunjukkan oleh pemberian ekstrak buah delima merah pada konsentrasi 56 ppm. Bahkan, pemberian ekstrak buah delima merah konsentrasi 56 ppm mampu meningkatkan kadar SOD hingga lebih tinggi daripada kelompok kontrol negatif (p = < 0,05), sedangkan pemberian ekstrak buah delima merah pada dengan konsentrasi 14 ppm dan 28 ppm, belum mampu meningkatkan kadar SOD secara signifikan (p = > 0,05). Sehingga dapat disimpulkan, bahwa pemberian ekstrak buah delima merah (Punica granatum) terbukti dapat meningkatkan kadar SOD pada kultur HUVECs yang dipapar plasma preeklampsi.   Kata kunci : delima merah, SOD dan Preeklamps

Red Pomegranate Extracts on Catalase Levels in Huvecs Culture Which are Exposed Preeclampsia Plasma

In a normal pregnancy, there is an increase in the production of free radicals, compared to on not pregnant and when the preeclampsia occurs the production is even more. This situation controlled with anti-oxidants. In general, antioxidants divided into two groups, namely: enzymatic antioxidants / primary antioxidants / antioxidant deterrents and nonenzymatic antioxidants consist of SOD, GPx and CAT. The purpose of this study is to determine the effect of giving Pomegranate Fruit extract to CAT levels in HUVECs who exposed to plasma preeclampsia. This study used a laboratory exploration method with samples: 1 negative control group (HUVECs culture exposed to normal 2% plasma pregnancy). One positive control group (HUVECs culture exposed to 2% preeclamptic plasma) and three treatment groups (HUVECs culture exposed to plasma 2% preeclampsia was given red pomegranate extract at different doses of 14 ppm, 28 ppm, 56 ppm, followed by calculating the amount of CAT which was a marker of intracellular antioxidants. Using the assumption of normality and homogeneity of variance tests that was using the Levene test as a prerequisite for parametric statistical testing, (2) One Way ANOVA Test (F Test), and (3) Pearson correlation test. The result is Red Pomegranate extract can prevent a decrease in CAT levels at a dose of 56 ppm. In conclusion, the Pomegranate Fruit extract can increase CAT level

PELAKSANAAN PENINGKATAN PENGETAHUAN DAN KETERAMPILAN SENAM HAMIL OLEH SELURUH BIDAN DI DESA PADA WILAYAH PUSKESMAS KARANG INTAN 2 TAHUN 2022

Angka Kematian Ibu di Kabupaten Banjar tahun 2019 turun dibandingkan pada tahun 2018. Hal tersebut terlihat dari data dimana angka kematian ibu pada tahun 2018 sebesar 93,3 / 100.000 kelahiran hidup dengan jumlah kasus 10 orang sedangkan tahun 2019 sebesar 83 / 100.000 kelahiran hidup dengan jumlah kasus 5 orang. Puskesmas Karang Intan 2 mengalami pengulangan kasus 1 kematian ibu di tahun 2018 dan 1 kematian ibu di tahun 2019 disebabkan oleh perdarahan karena robekan perineum. Salah satu hal untuk mencegah terjadinya robekan perineum yaitu dengan menjaga keelastisan perineum. Peningkatan elastisitas perineum dapat dilakukan dengan senam hamil. Senam hamil ialah suatu bentuk latihan guna memperkuat dan mempertahankan elastisitas otot-otot dinding perut, ligament-ligament, otot dasar panggul yang berhubungan dengan proses persalinan

PENDAMPINGAN IBU HAMIL RESIKO TINGGI PAPARAN DEBU BATUBARA DI DESA BUNTU KARAU KABUPATEN BALANGAN

Bayi berat lahir rendah (BBLR) adalah berat badan saat lahir kurang dari 2500 gram. WHO mencatat bahwa Indonesia berada diperingkat ke-9 dunia dengan persentasi >15,5% dari kelahiran bayi setiap tahunnya. Data Riset Kesehatan Dasar (Riskesdas) tahun 2018 menunjukan bahwa proporsi BBLR sebanyak 6,2%.3 Kasus BBLR di Kalimantan Selatan sebanyak 4,53%.4 Data Dinas Kesehatan Kabupaten Balangan tahun 2018 menunjukkan bahwa pada tahun 2017 persentase BBLR mengalami kenaikan sebesar 3,4% dibandingkan dengan tahun 2016 yaitu sebesar 3,3%. Kasus BBLR terbanyak di Kabupaten Balangan adalah wilayah kerja Puskesmas Margasari yaitu terdapat kasus BBLR sebanyak 7,5%, kasus ini meningkat dari tahun sebelumnya yaitu sebesar 2,2%. Bayi berat lahir rendah (BBLR) akan meningkatkan angka morbiditas dan mortalitas bayi. BBLR dapat  menyebabkan risiko infeksi yang tinggi, kekurangan gizi, kondisi cacat pada masa kanak-kanak, risiko penyakit degenerative ketika beranjak dewasa, dan masalah-masalah lain yang berkaitan dengan produktivitas anak. Wilayah Desa Buntu Karau Kecamatan Juai Kabupaten Balangan terkena dampak langsung dari kegiatan operasional pertambangan, merupakan lokasi dari keberadaan fasilitas utama perusahaan, dimana masyarakat memiliki frekuensi hubungan tinggi dengan perusahaan. Lingkupnya adalah satu atau beberapa desa yang wilayah atau area pencarian hidupnya terkena dampak langsung dari kegiatan perusahaan, baik yang bersifat dampak lingkungan dan social berdasarkan studi baseline (pendahuluan) dan Amdal (Analisa mengenai dampak lingkungan)  sehingga masuk dalam kriteria Ring 1 untuk daerah tambang Batubar

Effects of Otarian Gymnastics on Malondialdehyde (GoM) Levels in Postpartum Women

If a woman is in a physiological postpartum condition, her body will increase the production of free radicals. This situation will be worse if the woman is undergoing pathological postpartum, the production of free radicals in her body will be even greater. This condition is known as oxidative stress. This situation can be prevented by postpartum mothers doing Otaria exercises. The design used in this research was Post Test Only Control Group Design or Post-test with an experimental group of 20 respondents and 20 respondents in a control group of 20 respondents. The research was conducted in the PKM Karang Intan 2 working area, Banjar Regency, South Kalimantan Province, which includes 13 villages. The research instrument or measuring tool used to see the effect of exercise on MDA levels in postpartum mothers is the GoM Assay Kit (TBA method) from Assay Genie with product catalog: MAES0037. In the treatment group with a low average GoM, it can be seen that the average GoM shows an increase in the control group. This shows that Otaria exercise is able to reduce GoM levels significantly. Oxidative stress can be prevented with antioxidants as a mechanism for the body's defense system against free radicals. Oxidative stress in mothers during the post-partum period can be prevented by doing Otaria exercises which have been proven to be able to reduce GoM levels in post-partum maternal plasm

PENYEGARAN BIDAN DI DESA DALAM PENGETAHUAN DAN KETERAMPILAN SENAM HAMIL DI PUSKESMAS KARANG INTAN 2 TAHUN 2021

Angka Kematian Ibu di Kabupaten Banjar tahun 2019 turun dibandingkan pada tahun 2018. Hal tersebut terlihat dari data dimana angka kematian ibu pada tahun 2018 sebesar 93,3 / 100.000 kelahiran hidup dengan jumlah kasus 10 orang sedangkan tahun 2019 sebesar 83 / 100.000 kelahiran hidup dengan jumlah kasus 5 orang. Puskesmas Karang Intan 2 mengalami pengulangan kasus 1 kematian ibu di tahun 2018 dan 1 kematian ibu di tahun 2019 disebabkan oleh perdarahan karena robekan perineum. Salah satu hal untuk mencegah terjadinya robekan perineum yaitu dengan menjaga keelastisan perineum. Peningkatan elastisitas perineum dapat dilakukan dengan senam hamil. Senam hamil ialah suatu bentuk latihan guna memperkuat dan mempertahankan elastisitas otot-otot dinding perut, ligament-ligament, otot dasar panggul yang berhubungan dengan proses persalinan

Association of Obesity, Parity, and History of Knee Injury with Knee Osteoarthritis in Female

Background: Osteoarthritis is the most joint disorder among elderly. There are a lot of risk factors of knee osteoarthritis. Obesity and high frequent of parity can increase the load on knee joint. Knee injury also decreases the stability of knee joint on bearing body load. This study was conducted to observe the association of obesity, parity ≥3 times and history of knee injury with the occurrence of knee  osteoarthritis in female aged >50 years old.Methods: Case control design was chosen in this study. Female patients with age >50 years were recruited by consecutive sampling from rheumatology clinic at Dr. Hasan Sadikin General Hospital Bandung. Data were collected from August to September 2013. Minimal sample size was 60 samples calculated by rule of thumb formula. Obesity was measured by body mass index (BMI). Parity and history of knee injury were assessed by questionnaire. Those variables were analyzed by logistic regression method.Result: Obesity did not associate with knee osteoarthritis in this study, p=0.549 (odd ratio (OR) 1.32, 95% confidence interval (CI) 0.52–3.32). Parity was associated with knee osteoarthritis in this study p=0.001 (OR 4.7, 95% CI 1.89–11.68). History of knee injury was associated with knee osteoarthritis in this study, p=0.001 (OR 6.19, 95% CI 2.01–18.99).Conclusions: Parity ≥3 times and history of knee injury were associated with the occurrence of knee osteoarthritis. Obesity was not associated with the occurrence of knee osteoarthritis. [AMJ.2015;2(4):492–6] DOI: 10.15850/amj.v2n4.63

Relation between Preinfarction Angina and Coronary Collateral Circulation in Patients with Acute Myocardial Infarction

Background: Coronary collateral circulation conduits an alternative blood flow to the ischemic myocardium in the setting of coronary artery occlusion which can prevent the infarction area to extend more widely. Well-developed coronary collaterals are closely related with the presence of preinfarction angina. However, the duration of preinfarction angina which can induce well-developed coronary collateralization is in controversy. The aim of this study was to evaluate the relation between duration of preinfarction angina and coronary collaterals circulation in patients with acute myocardial infarction.Methods: This cross-sectional study was conducted from May to November 2013 in Dr. Hasan Sadikin General Hospital, Bandung, Indonesia. Seventy three acute myocardial infarction (AMI) patients were included in the study. The patients were divided into Group 1 (<7 days) and Group 2 (≥7 days) based on their preinfarction angina history. The coronary collaterals were assesed and graded as good (Rentrop score 2−3) and poor (Rentrop score 0−1).Statistical analysis was performed using the chi-square test.Result: The presence of a well-developed coronary collateral was not significantly different in <7days than ≥7 days duration of preinfarction angina [50.8% v 75.0%, p=0.124].Conclusions: There is no relation between the duration of preinfarction angina and coronary collaterals circulation in patients with acute myocardial infarction. [AMJ.2016;3(1):28–33]DOI: 10.15850/amj.v3n1.70

Profile of Blood Transfusion Requests from Hospitals to Bandung Blood Transfusion Unit, Indonesian Red Cross in 2011

Background: Blood transfusion as a part health services should be provided under appropriate indications and in a safe manner. In Indonesia, blood collection is run by the Blood Transfusion Unit of Indonesian Red Cross, where the blood is screened, processed into blood components, and finally distributed to hospitals. The purpose of this study was to describe the profile of blood transfusion requests from hospitals that do not have blood bank facility to the Bandung Blood Transfusion Unit, Indonesian Red Cross.Methods: A descriptive study was carried out using secondary data from Bandung Blood Transfusion Unit Indonesian Red Cross (UTD PMI Bandung).  All blood request forms from hospitals during 2011 were collected and analyzed. Variables in this study were the amount of  blood units, blood components, blood type, and indications of blood transfusion.Results: The number of blood units requested by hospitals were 35,841 units. The most blood units requested was in August 2011. The blood component requested was the packed red cell (61.1%), whole blood (17.4%), thrombocyte concentrate (10.6%), and fresh frozen plasma (7%). The total percentage of O, A, B and AB blood types were 36.1%, 28.6%, 27.5%, and 7.9% respectively. The most frequent indication for transfusion was anemia (61.7%), followed by surgery and other causes of bleeding.  Conclusions: The total blood units requested by hospitals vary each month. The most blood component requested is Packed Red Cell and the type of blood is O blood type. The most frequent indication is anemia. DOI: 10.15850/amj.v2n3.51