26,902 research outputs found
A DAQ System for Linear Collider TPC Prototypes based on the ALEPH TPC Electronics
Within the international studies of a high energy linear electron positron
collider, several groups are developing and testing prototypes for a Linear
Collider TPC. This detector is planned to be used as a central part in the
tracking system of a detector at such a machine. In this note we describe a DAQ
system, which has been developed for the use in tests of TPC prototypes. It is
based on electronics used at the ALEPH experiment at CERN.Comment: 15 pages, 4 figure
Specificity and Kinetics of Haloalkane Dehalogenase
Haloalkane dehalogenase converts halogenated alkanes to their corresponding alcohols. The active site is buried inside the protein and lined with hydrophobic residues. The reaction proceeds via a covalent substrate-enzyme complex. This paper describes a steady-state and pre-steady-state kinetic analysis of the conversion of a number of substrates of the dehalogenase. The kinetic mechanism for the “natural” substrate 1,2-dichloroethane and for the brominated analog and nematocide 1,2-dibromoethane are given. In general, brominated substrates had a lower Km, but a similar kcat than the chlorinated analogs. The rate of C-Br bond cleavage was higher than the rate of C-Cl bond cleavage, which is in agreement with the leaving group abilities of these halogens. The lower Km for brominated compounds therefore originates both from the higher rate of C-Br bond cleavage and from a lower Ks for bromo-compounds. However, the rate-determining step in the conversion (kcat) of 1,2-dibromoethane and 1,2-dichloroethane was found to be release of the charged halide ion out of the active site cavity, explaining the different Km but similar kcat values for these compounds. The study provides a basis for the analysis of rate-determining steps in the hydrolysis of various environmentally important substrates.
Onderzoek naar de klantwachttijd in relatie met de personeelsinzet bij de ANWB-wegenwacht district Zuid
Spins in organic semiconductors : revealing the dominant mechanisms of organic magnetoresistance
Unsupervised image segmentation with neural networks
The segmentation of colour images (RGB), distinguishing clusters of image points, representing for example background, leaves and flowers, is performed in a multi-dimensional environment. Considering a two dimensional environment, clusters can be divided by lines. In a three dimensional environment by planes and in an n-dimensional environment by n-1 dimensional structures. Starting with a complete data set the first neural network, represents an n-1 dimensional structure to divide the data set into two subsets. Each subset is once more divided by an additional neural network: recursive partitioning. This results in a tree structure with a neural network in each branching point. Partitioning stops as soon as a partitioning criterium cannot be fulfilled. After the unsupervised training the neural system can be used for the segmentation of images
Drived diffusion of vector fields
A model for the diffusion of vector fields driven by external forces is
proposed. Using the renormalization group and the -expansion, the
dynamical critical properties of the model with gaussian noise for dimensions
below the critical dimension are investigated and new transport universality
classes are obtained.Comment: 11 pages, title changed, anisotropic diffusion further discussed and
emphasize
Influence of mutations of Val226 on the catalytic rate of haloalkane dehalogenase
Haloalkane dehalogenase converts haloalkanes to their corresponding alcohols. The 3D structure, reaction mechanism and kinetic mechanism have been studied. The steady state kcat with 1,2-dichloroethane and 1,2-dibromoethane is limited mainly by the rate of release of the halide ion from the buried active-site cavity. During catalysis, the halogen that is cleaved off (Clα) from 1,2-dichloroethane interacts with Trp125 and the Clβ interacts with Phe172. Both these residues have van der Waals contacts with Val226. To establish the effect of these interactions on catalysis, and in an attempt to change enzyme activity without directly mutating residues involved in catalysis, we mutated Val226 to Gly, Ala and Leu. The Val226Ala and Val226Leu mutants had a 2.5-fold higher catalytic rate for 1,2-dibromoethane than the wild-type enzyme. A pre-steady state kinetic analysis of the Val226Ala mutant enzyme showed that the increase in kcat could be attributed to an increase in the rate of a conformational change that precedes halide release, causing a faster overall rate of halide dissociation. The kcat for 1,2-dichloroethane conversion was not elevated, although the rate of chloride release was also faster than in the wild-type enzyme. This was caused by a 3-fold decrease in the rate of formation of the alkyl-enzyme intermediate for 1,2-dichloroethane. Val226 seems to contribute to leaving group (Clα or Brα) stabilization via Trp125, and can influence halide release and substrate binding via an interaction with Phe172. These studies indicate that wild-type haloalkane dehalogenase is optimized for 1,2-dichloroethane, although 1,2-dibromoethane is a better substrate.
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