In vivo X-ray elemental imaging of single cell model organisms manipulated by laser-based optical tweezers

We report on a radically new elemental imaging approach for the analysis of biological model organisms and single cells in their natural, in vivo state. The methodology combines optical tweezers (OT) technology for non-contact, laser based sample manipulation with synchrotron radiation confocal X-ray fluorescence (XRF) microimaging for the first time. The main objective of this work is to establish a new method for in vivo elemental imaging in a two-dimensional (2D) projection mode in free-standing biological microorganisms or single cells, present in their aqueous environment. Using the model organism Scrippsiella trochoidea, a first proof of principle experiment at beamline ID13 of the European Synchrotron Radiation Facility (ESRF) demonstrates the feasibility of the OT XRF methodology, which is applied to study mixture toxicity of Cu-Ni and Cu-Zn as a result of elevated exposure. We expect that the new OT XRF methodology will significantly contribute to the new trend of investigating microorganisms at the cellular level with added in vivo capability

Inferior outcome of addition of the aminopeptidase inhibitor tosedostat to standard intensive treatment for elderly patients with aml and high risk mds

Treatment results of AML in elderly patients are unsatisfactory. We hypothesized that addition of tosedostat, an aminopeptidase inhibitor, to intensive chemotherapy may improve outcome in this population. After establishing a safe dose in a run-in phase of the study in 22 patients, 231 eligible patients with AML above 65 years of age (median 70, range 66–81) were randomly assigned in this open label randomized Phase II study to receive standard chemotherapy (3+7) with or without tosedostat at the selected daily dose of 120 mg (n = 116), days 1–21. In the second cycle, patients received cytarabine 1000 mg/m2 twice daily on days 1-6 with or without tosedostat. CR/CRi rates in the 2 arms were not significantly different (69% (95% C.I. 60–77%) vs 64% (55–73%), respectively). At 24 months, event-free survival (EFS) was 20% for the standard arm versus 12% for the tosedostat arm (Cox-p = 0.01) and overall survival (OS) 33% vs 18% respectively (p = 0.006). Infectious complications accounted for an increased early death rate in the tosedostat arm. Atrial fibrillation w

Endocriene aspecten van de o6genese bij enkele insectensoorten

Available from KULeuven, Campusbib. Exacte Wetenschappen, Celestijnenlaan 300A, 3001 Heverlee, Belgium / UCL - Université Catholique de LouvainSIGLEBEBelgiu

Application of Bayesian Networks in the development of herbs and spices sampling monitoring system

Knowing which products and hazards to monitor along the food supply chain is crucial for ensuring food safety. In this study, we developed a model to predict which types of herbs and spices products and food safety hazards should preferentially be monitored at each level of the supply chain (suppliers, border inspection points, market and consumers). A Bayesian Network method was used to develop a model based on notifications reported in the Rapid Alert System for Food and Feed and the database of the Dutch national monitoring program for chemical contaminants in food and feed over the period 2005-2014. The model was constructed by randomly selecting ca. 80% of the 3126 data records and validated using the remaining ca. 20% of the records. Model validation showed that the prediction accuracy was higher than 85%. Results showed that the sampling plan is closely related to the place where the products are checked along the supply chain, the products and the country of origin. Our approach of integrating different data sources and considering the entire supply chain can support industry and authorities at border inspection points and at all control points along the herbs and spices supply chain in setting priorities for their monitoring program

Social Media: A Harsher Reality for Adolescents with Depression

Social media use is often highlighted as an important cause of the recent rise in depression among adolescents. However, this perspective overlooks a crucial reverse causality, namely that levels of depression might also shape adolescents’ social media use. In a diary study among 479 adolescents (Mage = 15.98; 16.9% clinically depressed), we assessed their level of depression and then monitored their social media activities and experiences daily for 100 subsequent days. Depressed adolescents did not differ from their non-depressed peers in the frequency of posting and the time spent scrolling. However, they reported feeling twice as insecure after scrolling, nearly twice as rejected during online communication with friends, and significantly more preoccupied with feedback. Our findings underscore the need for further research into the mechanisms of how depressive symptoms influence adolescents’ social media experiences, to inform the development of effective digital tools and therapeutic approaches to support adolescent mental health

Amaranth (Amaranthus spp.), buckwheat (Fagopyrum spp.), and quinoa (Chenopodium spp.) proteins: a food science and technology perspective

The use of pseudocereals as raw materials for developing nutritious food products is skyrocketing. Pseudocereals are dicotyledonous crops - unlike true cereals, which are monocotyledonous - with starch-rich seeds. The three major pseudocereals in terms of world production are amaranth, buckwheat, and quinoa. Apart from starch, they contain high levels of protein, dietary fiber, minerals, vitamins, and other bioactive constituents. Their proteins have well-balanced amino acid compositions, are more sustainable than those from animal sources, and can safely be incorporated in the diet of celiac patients. While prolamins and glutelins are the most important protein fractions of true cereals, the proteins of pseudocereals are predominantly albumins and globulins. The traditional Osborne fractionation scheme is not always rigorously followed for pseudocereals, particularly in the methods used to extract globulins and glutelins. It is also of note here that, in order to establish in-depth structure-function relationships, future research should aim at (i) unlocking the genome sequence of pseudocereal proteins and (ii) unraveling the primary, secondary, tertiary, and quaternary structures of amaranth, buckwheat, and quinoa proteins. Basic foaming, emulsifying, and gelling properties of pseudocereal proteins are often similar to or even better than those of industrially relevant protein sources such as casein, which makes them potential substitutes for animal proteins in many food applications. It is, however, imperative to emphasize that, in most studies concerning the functionality of pseudocereal proteins, the protein isolates were obtained by alkaline extraction and subsequent isoelectric precipitation at acidic pH. Such harsh extraction conditions provoke protein denaturation and thus alter the native protein conformation and, as a consequence, the techno-functional properties. Increasing knowledge on structural changes in the native proteins from amaranth, buckwheat, and quinoa during processing steps relevant to food production and the effect thereof on their techno-functional properties is essential to expand the prospects for exploitation of pseudocereals.status: accepte

Incentives to stimulate European wheat farmers to adapt their Fusarium species mycotoxin management

A questionnaire was distributed amongst wheat farmers from Italy, the Netherlands, Serbia, and the United Kingdom. Using the respondents’ data, Bayesian Network modelling was applied to estimate the probability that farmers would adapt their current agronomic management under eight different incentives given the conditions set by their farm and farmer characteristic

Meesters in bouwen dankzij Bouwmeester

Open brief als protest tegen de afschaffing van het bouwmeesterschap.status: publishe

Baculovirus immediate early gene promoter based expression vectors for transient and stable transformation of insect cells

A recombinant plasmid vector was constructed in which the bacterial LacZ gene was placed under the control of a Bombyx mori baculovirus early promoter. The vector proved to be active in transfected cultured dipteran and lepidopteran cells. Co-transfection carried out with this recombinant plasmid vector and a plasmid containing the hygromycin phosphotransferase gene followed by selection with the antibiotic hygromycin B, resulted in stable transformation of cultured Drosophila melanogaster Schneider 2 cells. Southern blot analysis of the host cell's genomic DNA in combination with chromosomal in situ hybridization demonstrated that multiple copies of both plasmids were integrated in the host cell's genome.status: publishe

Proteins of Amaranth (Amaranthus spp.), Buckwheat (Fagopyrum spp.), and Quinoa (Chenopodium spp.): A Food Science and Technology Perspective

© 2016 Institute of Food Technologists® There is currently much interest in the use of pseudocereals for developing nutritious food products. Amaranth, buckwheat, and quinoa are the 3 major pseudocereals in terms of world production. They contain high levels of starch, proteins, dietary fiber, minerals, vitamins, and other bioactives. Their proteins have well-balanced amino acid compositions, are more sustainable than those from animal sources, and can be consumed by patients suffering from celiac disease. While pseudocereal proteins mainly consist of albumins and globulins, the predominant cereal proteins are prolamins and glutelins. We here discuss the structural properties, denaturation and aggregation behaviors, and solubility, as well as the foaming, emulsifying, and gelling properties of amaranth, buckwheat, and quinoa proteins. In addition, the technological impact of incorporating amaranth, buckwheat, and quinoa in bread, pasta, noodles, and cookies and strategies to affect the functionality of pseudocereal flour proteins are discussed. Literature concerning pseudocereal proteins is often inconsistent and contradictory, particularly in the methods used to obtain globulins and glutelins. Also, most studies on protein denaturation and techno-functional properties have focused on isolates obtained by alkaline extraction and subsequent isoelectric precipitation at acidic pH, even if the outcome of such studies is not necessarily relevant for understanding the role of the native proteins in food processing. Finally, even though establishing in-depth structure–function relationships seems challenging, it would undoubtedly be of major help in the design of tailor-made pseudocereal foods.status: publishe