4 research outputs found
The effects of anthocyaninrich wheat diet on the oxidative status and behavior of rats
Aim To evaluate the effect of food containing anthocyanin-
rich wheat on oxidative status and behavior of healthy
rats.
Methods Twenty male rats were divided into the control
and anthocyanin group. Oral glucose tolerance test was
performed, and proteinuria and creatinine clearance were
measured. Behavioral analysis was performed in Phenotyper
cages. Serum and tissues were collected to measure
the markers of oxidative stress and antioxidant status.
Results Anthocyanins significantly increased total antioxidant
capacity in serum (P = 0.039), decreased advanced
oxidation protein products in the kidney (P = 0.002), but
increased thiobarbituric acid reactive substances in the
kidney compared to the control group. No significant difference
between the groups was found in the markers
of oxidative stress in the liver and colon, as well as in renal
functions and glucose metabolism. The anthocyanin
group spent significantly less time in the spotlight zone of
the Phenotyper cages (P = 0.040), indicating higher anxiety-
like behavior.
Conclusion Food containing anthocyanin-rich wheat had
positive effects on serum antioxidant status and kidney
protein oxidation, but increased lipid peroxidation in the
kidney and modified animal behavior related to anxiety.
The molecular mechanisms leading to observed effects
should be further elucidated
Oxidative Stress in Animal Models of Acute and Chronic Renal Failure
Introduction. Kidney disease is a worldwide health and economic burden, with rising prevalence. The search for biomarkers for earlier and more effective disease screening and monitoring is needed. Oxidative stress has been linked to both, acute kidney injury (AKI) and chronic kidney disease (CKD). The aim of our study was to investigate whether the concentrations of systemic markers of oxidative stress and antioxidant status are affected by AKI and CKD, and to identify potential biomarkers. Methods. In adult male Wistar rats, AKI was induced by bilateral nephrectomy, and CKD was induced by 5/6 nephrectomy. Blood was collected 48 hours after surgery in AKI and 6 months after surgery in CKD. Advanced oxidation protein products (AOPP), thiobarbituric acid reactive substances (TBARS), advanced glycation end products (AGEs), fructosamine, total antioxidant capacity (TAC), and ferric reducing antioxidant power (FRAP) were measured. Results. Impaired renal function was confirmed by high concentrations of plasma creatinine and urea in AKI and CKD animals. AOPP and fructosamine were higher by 100% and 54% in AKI, respectively, and by 100% and 199% in CKD, respectively, when compared to corresponding control groups. Similarly, there was approximately a twofold increase in AGEs (by 92%) and TAC (by 102%) during AKI. In CKD, concentrations of FRAP, as an antioxidative status marker, were doubled (by 107%) when compared to the control group, but concentration of TAC, another marker of antioxidative status, did not differ between the groups. Conclusions. AKI and CKD led to increased systemic oxidative stress. AOPP and fructosamine could be considered potential biomarkers for both, acute and chronic kidney damage. On the other hand, AGEs, TAC, and FRAP seem to be disease specific, which could help to differentiate between acute and chronic kidney injuries. However, this needs further validation in clinical studies