854 research outputs found

    Functional characterization of human CAP2.

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    Essential cellular processes such as cell motility, cell migration and endocytosis/exocytosis require a dynamic actin cytoskeleton. Actin filaments (F-actin) polymerize and depolymerize into monomeric actin (G-actin) in response to signals from the environment. Several different signaling pathways lead to changes in the actin cytoskeleton (e.g. Rac, RhoA, Cdc42). Cyclase Associated Proteins (CAPs) interact with monomeric actin and are conserved in many species. Humans have two CAP genes, CAP1 and CAP2. Previous studies have demonstrated a role for CAPs in regulating the actin cytoskeleton and a role in endocytosis/exocytosis through sequestration of actin monomers. The objective of this study was to determine the localization and regulation of human CAP2. Deletion and site directed mutagenesis mutants were constructed and immunoprecipitation reactions were performed with wild type and mutant HA epitope tagged CAP2. (Abstract shortened by UMI.)Dept. of Science. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis2003 .S85. Source: Masters Abstracts International, Volume: 43-05, page: 1669. Adviser: Andrew Hubberstey. Thesis (M.Sc.)--University of Windsor (Canada), 2004

    Synaptic Vesicles Caught Kissing Again

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    In this issue of Neuron, Harata et al. use a novel quenching technique to provide compelling evidence that kiss-and-run is the dominant mode of vesicle fusion at hippocampal synapses and that the prevalence of kiss-and-run can be modulated by stimulus frequency. The increased incidence of kiss-and-run at lower frequencies may ensure that vesicles are available for use during periods of high demand

    Environmental, ecological, and fishery effects on growth and size-at-age of Pacific halibut (Hippoglossus stenolepis)

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    Thesis (M.S.) University of Alaska Fairbanks, 2016Size-at-age of Pacific Halibut (Hippoglossus stenolepis) has declined significantly since the 1980s. For instance, the average weight of a 20-year-old female declined from 55 kg in 1988 to 20 kg in 2014. The decline in size-at-age corresponds to a period of declining Pacific Halibut recruitment, spawning biomass, and reductions in catch limits for the directed commercial Pacific Halibut longline fishery. The causes of changes in Pacific Halibut size-at-age are poorly understood. Our project investigates several hypotheses related to declines in size-at-age, including the effects of environmental and ecological variability on growth, and the cumulative effects of harvest and size-selective fishing. Specific potential environmental covariates include the Pacific Decadal Oscillation, which is an index of basin-wide sea surface temperatures, and summer sea surface temperatures along the continental shelf of the Gulf of Alaska. Specific ecological variables include annual biomass estimates of Arrowtooth Flounder (Atheresthes stomias) and Pacific Halibut to investigate the potential role of inter- and intraspecific competition, respectively. We used a population modeling approach to simulate the effects of fishing on size-at-age. We found that the large increase in Arrowtooth Flounder biomass since the 1970s corresponds to declines in Pacific Halibut size-at-age. Our results also suggest that periods of high Pacific Halibut biomass relates to poor growth and low size-at-age. Finally, we found that harvest and size-selective fishing explains between 30 and 65% of observed declines since the 1980s in the Gulf of Alaska, and up to 100% of the declines in Southeast Alaska and British Columbia where harvest rates were high in the 1990s and 2000s. Our findings have implications for fisheries management, including balancing tradeoffs associated with size limits, and understanding how changes in environmental and ecological conditions can shift management reference points such as maximum sustainable yield

    Preferences for Male or Female Counselors as Related to Student-Counselee Sex, Grade Level, Type of Presenting Concern and Self-Esteem

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    Problem: The purpose of this study was to investigate the relationship between the selected variables of student-counselee -- sex, grade level, and self-esteem -- and the preference for a male or a female counselor by types of presenting concerns. Procedure: The subjects of this study consisted of 375 students, 189 males and 186 females, enrolled in a junior-senior high school in a rural community in north central Minnesota The subjects were all adolescents in grades seven through twelve, inclusive. The sample size for each grade level ranged from a minimum of sixty to a maximum of sixty-nine. The instruments used in this study were a personal data questionnaire and the Coopersmith Self-Esteem Inventory. The statistical tests employed included multiple regression analysis, chi square and canonical correlation. The .05 level of confidence was used, where applicable, to evaluate the significance of the obtained results Findings: The major findings of this study were as follows: 1. There was a significant difference in the preference for a male or a female counselor by type of presenting concern and the sex of the student-counselee. A preference existed for a female counselor by both sexes for the concern areas of health-physical, homeschool, boy-girl, moral-religious, and personal-social, while a preference existed for a male counselor for the school and vocational educational concern areas Significant proportional differences of males and females in counselor choice existed in all concern areas except for the school concern area, where no significant difference was found. 2. There was no significant relationship between the preference for a male or a female counselor by type of presenting concern and the grade level of the student-counselee. 3. There was no significant relationship between the preference for a male or a female counselor when type of presenting concern and the self-esteem of the student-counselee were considered. 4. There were significant relationships between the first set of variables measuring student-counselee presenting concerns and the second set of variables of student-counselee sex, grade level, .age and self-esteem Health-physical concerns from the first set and sex of the student-counselee from the second set contributed most to the canonical correlation. Conclusions: Female counselors were preferred for all personal-social concern areas and male counselors were preferred for school and vocational educational concerns with that preference increasing with each succeeding grade.· In addition, self-esteem was not found to be an important variable in determining counselor preference. An additional conclusion was that student-counselee sex and the health-physical concern made the most important contribution to the canonical correlation Finally, it was suggested that rising social consciousness may have altered stereotypic perceptions of women\u27s roles as evidenced by the preference for a female counselor for all personal and social concern areas. Recommendations: Several recommendations were offered for further research to promote a better understanding of preferences for male or female counselors. 1. A replication of the study was recommended using other populations to determine if the results of this study may be generalized. 2. It was recommended that the self-esteem variable be explored in conjunction with the preference for a male or a female counselor where students have actually made a counselor choice. 3. It was further recommended that school boards hire female counselors, in appropriate numbers, in order to allow for choice by student-counselees

    The journey from provider to patient: Lessons learned

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    The lens through which one experiences a health crisis is influenced by their personal beliefs and experiences as well as by their professional training and circumstances. Parotid (salivary) gland tumors are rare. Having more than one parotid tumor is rarer still. My experience as a clinician and researcher who was diagnosed with two parotid gland tumors was influenced by my life experiences and my professional background. This affected my reaction to the situation and the questions I asked. It also affected my expectations of my providers. This is a reflection on my six-plus-year journey and the lessons learned, both personally and professionally. The journey will be a lifelong one, hopefully full of promise and health. Experience Framework This article is associated with the Patient, Family & Community Engagement lens of The Beryl Institute Experience Framework (https://theberylinstitute.org/experience-framework/). Access other PXJ articles related to this lens. Access other resources related to this lens

    Pregnancy, prison and perinatal outcomes in New South Wales, Australia: a retrospective cohort study using linked health data

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    BACKGROUND Studies from the United States and the United Kingdom have found that imprisoned women are less likely to experience poorer maternal and perinatal outcomes than other disadvantaged women. This population-based study used both community controls and women with a history of incarceration as a control group, to investigate whether imprisoned pregnant women in New South Wales, Australia, have improved maternal and perinatal outcomes. METHODS Retrospective cohort study using probabilistic record linkage of routinely collected data from health and corrective services in New South Wales, Australia. Comparison of the maternal and perinatal outcomes of imprisoned pregnant women aged 18-44 years who gave birth between 2000-2006 with women who were (i) imprisoned at a time other than pregnancy, and (ii) community controls. OUTCOMES OF INTEREST onset of labour, method of birth, pre-term birth, low birthweight, Apgar score, resuscitation, neonatal hospital admission, perinatal death. RESULTS Babies born to women who were imprisoned during pregnancy were significantly more likely to be born pre-term, have low birthweight, and be admitted to hospital, compared with community controls. Pregnant prisoners did not have significantly better outcomes than other similarly disadvantaged women (those with a history of imprisonment who were not imprisoned during pregnancy). CONCLUSIONS In contrast to the published literature, we found no evidence that contact with prison health services during pregnancy was a "therapunitive" intervention. We found no association between imprisonment during pregnancy and improved perinatal outcomes for imprisoned women or their neonates. A history of imprisonment remained the strongest predictor of poor perinatal outcomes, reflecting the relative health disadvantage experienced by this population of women.This work was undertaken with funding from the National Health and Medical Research Council of Australia. Project Grant ID 457515
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