Weather and Gremmeiella in Sweden

Weather and Gremmeiella in Swede

Amino acids at non-synonymous SNP sites in gene RGA1 for the parents and two grandparents of mapping population S₁.

<p>Amino acids at non-synonymous SNP sites in gene RGA1 for the parents and two grandparents of mapping population S₁.</p

Graphical representation the genomic location of the QTL and RGA1 gene.

<p>(a) Location of the QTL on the <i>Salix</i> linkage group S₁Ib. General similarities in chromosomal regions to <i>Populus trichocarpa</i> identified by Berlin et al. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0168776#pone.0168776.ref007" target="_blank">7</a>] are shown. Specific positions of markers XII-9_sa, XI_14om_sa and Ro_9_sa are highlighted. (b) Conservation between chromosome 17 regions in <i>Salix pupurea</i> and <i>P</i>. <i>trichocarpa</i>. Arrows represent hypothetical genes. A duplicated region containing ten genes (<i>A-J</i>) on poplar chromosome 17 is shown. Homologous genes in (b) and (c) are labelled with the same letter and/or colour. (c) <i>S</i>. <i>pupurea</i> Scaffold 0941 and <i>P</i>. <i>trichocarpa</i> chromosome 1 regions showing homologous genes (<i>K-N</i>). Function/products of genes <i>A-N</i>: <i>A</i> = Diacylglycerol acyltransferase, <i>B</i> = Transferase, <i>C</i> = Diacylglycerol acyltransferase, <i>D</i> = TIR-NBS-LRR resistance protein, <i>E</i> = Jacalin-like lectin domain, <i>F</i> = Ribosome inactivating protein, <i>G</i> = F-box domain, <i>H</i> = Plastocyanin-like domain, <i>I</i> = Transcription-coupled repair protein, <i>J</i> = Predicted transcriptional regulator, <i>K</i> = Transferase, <i>L</i> = IQ calmodulin-binding motif, <i>M</i> = Electron transporter, <i>N</i> = NAC transcription factor.</p

Alignment of RGA1 amino acid sequence from <i>Salix</i> genotypes ‘Björn’ (resistant) and ‘78183’ (susceptible).

<p>Predicted domains are highlighted. Non-synonymous SNPs are shown with the extent of the amino acid change indicated.</p

Gene expression data for gene RGA1 in susceptible (‘78183’) and resistant (‘Björn’) <i>Salix</i> genotypes when challenged with rust spores, at 6, 12, 24 and 48 hours post inoculation.

<p>Relative fold expression is shown for control and inoculated plants with three biological replicates for each sample and condition. * indicates significance at p = 0.05. Error bars indicate standard deviation. P-values for significant differences between genotypes and between treatments are indicated: * p<0.05; ** p<0.01; *** p<0.001.</p

Infection pattern on inoculated leaves from the gene expression experiment 13 days post inoculation.

<p>Leaves from the resistant <i>Salix</i> genotype ‘Björn’ to the left, and the susceptible genotype ‘78183’ to the right.</p

Lesion activity each month at 'the three sites'.

<p>Percentage of active lesions and the growth rate measured as the sum of extension (cm²) of all active lesions each month divided by number of active lesions.</p

Growth rate (mm/day) at different temperatures for seventeen isolates cultured on malt-agar media.

<p>Growth rate (mm/day) at different temperatures for seventeen isolates cultured on malt-agar media.</p

Number of lesions at the four localities each month.

<p>At Gnesta, Österbybruk and Åkersberga one lesion per tree was surveyed, and additional trees were included in the survey during 2008. At Ultuna, all lesions were included in the survey as they emerged on the tree. Lesions were lost during the survey due to the death of the tree or a part of the tree due to another lesion girdling the branch or shoot. At Ultuna, lesions were also lost due to fusion of lesions.</p

Percentage of active lesions each month at Gnesta, Österbybruk and Åkersberga.

<p>Percentage of active lesions each month at Gnesta, Österbybruk and Åkersberga.</p