Spectrofluorimetric Determination of Human Serum Albumin Using Terbium-Danofloxacin Probe

A spectrofluorimetric method is proposed for the determination of human serum albumin (HSA) and bovine serum albumin (BSA) using terbium-danofloxacin (Tb3+-Dano) as a fluorescent probe. These proteins remarkably enhance the fluorescence intensity of the Tb3+-Dano complex at 545 nm, and the enhanced fluorescence intensity of Tb3+-Dano is proportional to the concentration of proteins (HSA and BSA). Optimum conditions for the determination of HSA were investigated and found that the maximum response was observed at: pH = 7.8, [Tb3+] = 8.5 × 10−5 mol L−1, [Dano] = 1.5 × 10−4 mol L−1. The calibration graphs for standard solutions of BSA, HSA, and plasma samples of HSA were linear in the range of 0.2 × 10−6 − 1.3 × 10−6 mol L−1, 0.2 × 10−6 − 1.4 × 10−6 mol L−1, and 0.2 × 10−6 − 1 × 10−6 mol L−1, respectively. The detection limits (S/N = 3) for BSA, HSA, and plasma sample of HSA were 8.7 × 10−8 mol L−1, 6.2 × 10−8 mol L−1, and 8.1 × 10−8 mol L−1, respectively. The applicability of the method was checked using a number of real biological plasma samples and was compared with the UV spectrometric reference method. The results was showed that the method could be regarded as a simple, practical, and sensitive alternative method for determination of albumin in biological samples

Solubility of Anthracene in Binary and Ternary Mixtures of Cyclohexanone, Ethyl Acetate, and Methanol at 298.2 K

This article discusses the solubility of anthracene in binary and ternary mixtures of cyclohexanone, ethyl acetate, and methanol at 298.2 K

Solubility of Anthracene in C 1

Article discussing the solubility of anthracene in C1-C3 alcohols from (298.2 to 333.2) K and their mixtures with 2-propanone at 298.2 K

Solubility of Anthracene in C1-C3 Alcohols from (298.2 to 333.2) K and Their Mixtures with 2-Propanone at 298.2 K

Article discussing the solubility of anthracene in C1-C3 alcohols from (298.2 to 333.2) K and their mixtures with 2-propanone at 298.2 K

Spectrofluorimetric and cyclodextrin-enhanced spectrofluorimetric methods for the determination of naphazoline in nasal and eye drops

2988-2992A spectrofluorimetric method has been developed for the determination of naphazoline based on its fluorescence activity. This method allows the determination of 0.1-3.0μg ml-l naphazoline in aqueous solution with excitation and emission wavelengths at 281 and 330 nm respectively. The complexation between naphazoline and β-cyclodextrin gives a 1: 1 complex. A cyclodextrin-enhanced spectrofluorimetric method has also been developed which has better sensitivity and limit of detection (15ng ml-l) as compared to that in the absence of cyclodextrin. Both methods have been applied satisfactorily to the determination of naphazoline in pharmaceutical preparations

Development of a Simple and Sensitive Terbium SensitizedFluorescence Method forDetermination of Epinephrine andNorepinephrine in Serum: Determination of nor/epinephrine in serum

Asimple, rapid, selective and highly sensitive fluorimetric method fordetermination of two catecholamines, i.e. norepinephrine (NE) and epinephrine (EP),in serum samples was developed. The method is based on the fluorescencesensitization of terbium (Tb3+) by complexation with both catecholamines in thepresence of lanthanum (La3+), as a co-cation, and in a Na2SO3solution (chemicaldeoxygenating agent), which fluoresces intensely with an emission maximum at 545nm when excited at 312 nm. We found that fluorescence enhancement effects wereobserved when La3+was added to the system (co-luminescence effect). In thepresence of this element the fluorescence of the Tb-catecholamines system wasenhanced by a factor of about 15 compared with that of the system without La3+.Optimum conditions for the formation of the complexes were investigated. Underoptimum conditions, a linear relationship was obtained between the fluorescenceintensity and catecholamines concentration in the range of 2.5×10-3to 0.22 μg.ml-1. The detection limits were 0.59 and 0.58 μg.l-1for EPand NE, respectively.Relative standard deviation (RSD) values for two compounds were in the range of1.2-2.1% indicating excellent reproducibility. The proposed method was successfullyapplied to the determination of EPand NE in spiked serum samples after deproteiniza-tion of the samples with acetonitrile. Analytical recoveries from treated serumsamples were in the range of 95-105%

Spectrofluorimetric Determination of Doxorubicin in Spiked Serum and Urine Samples

.A simple spectrofluorimetric method is described for the determination of doxorubicin (DXR) based on its quenching effect on the fluorescence intensity of Tb3+- deferasirox (DFX) complex as a fluorescent probe. The excitation and emission wavelengths were 328 and 545 nm, respectively. The effects of pH, time, order of addition of reagents, concentrations of Tb3+ and DFX and the buffer volume on the quenched fluorescence intensity were investigated and optimized. In the optimum conditions, the decrease of the fluorescence intensity of the system showed a good linear relationship with the concentration of DXR in the range of 20-1000 μg L-1, with a correlation coefficient 0.998. The detection limit (3s) was 6.1 μg L-1 and the relative standard deviation for four replicate determinations of different concentrations of DXR was in the range of 1.7–4.4%. The procedure was successfully applied to the determination of doxorubicin in urine and serum samples</table

Spectrofluorimetric determination of cefixime using terbium-danofloxacin probe

Objective(s):Cefixime (Cfx), is a semi-synthetic third-generation oral cephalosporin antibiotic that is prescribed for the treatment of susceptible infections. There are some procedures for the determination of Cfx in pharmaceutical formulations and biological samples. Herein a spectrofluorimetric method was proposed for Cfx determination based on the fluorescence quenching of terbium-danofloxacin (Tb3+-Dano) in the presence of Cfx. Materials and Methods: Cfx was detected based on fluorescence quenching of terbium-danofloxacin (Tb3+-Dano) in the presence of Cfx with maximum excitation and emission wavelengths at 347 nm and 545 nm, respectively. The quenched fluorescence intensity of Tb3+- Dano system is proportional to the concentration of Cfx. The optimum conditions for the determination of Cfx were studied. Results: The maximum response was achieved under optimum conditions of [Tris buffer]= 0.008 mol/l (pH 6.5), [Tb3+]=1×10-4 mol/l  and [Dano]=1×10-4 mol/l. The developed method was evaluated in terms of accuracy, precision and limit of detection. The linear concentration ranges for quantification of Cfx were 8.8×10-8-8.8×10-7 mol/l and 1.1×10-7-8.8×10-7 mol/l in standard and human serum samples with the detection limits (S/N=3) of 2.8×10-8 mol/l and 3.9×10-8 mol/l, respectively. The Cfx was determined in pharmaceutical tablets and spiked serum samples and the results were satisfactory.   Conclusion: This method is simple, practical and relatively interference-free for determination of Cfx in pharmaceutical tablets and serum samples

Spectrofluorimetric Determination of Human Serum Albumin Using Terbium-Danofloxacin Probe

A spectrofluorimetric method is proposed for the determination of human serum albumin (HSA) and bovine serum albumin (BSA) using terbium-danofloxacin (Tb 3+ -Dano) as a fluorescent probe. These proteins remarkably enhance the fluorescence intensity of the Tb 3+ -Dano complex at 545 nm, and the enhanced fluorescence intensity of Tb 3+ -Dano is proportional to the concentration of proteins (HSA and BSA). Optimum conditions for the determination of HSA were investigated and found that the maximum response was observed at: pH = 7.8, [Tb 3+ , respectively. The applicability of the method was checked using a number of real biological plasma samples and was compared with the UV spectrometric reference method. The results was showed that the method could be regarded as a simple, practical, and sensitive alternative method for determination of albumin in biological samples