Níveis plasmáticos de TNF-alfa, TRAIL e FasLigante/CD95L correlacionados com a disfunção ventricular em pacientes com cardiopatia chagásica crônica Ouro Preto 2008.

Apoptose de cardiomiócitos tem sido reportado estar envolvido na patogênese da cardiomiopatia chagásica crônica (CCC). Para determinar se a maquinaria apoptótica está correlacionada aos distúrbios arrítmicos ventriculares e funcionais na CCC, foi examinado os ligantes da superfamília do TNF (TNF-alfa, Fasligante/CD95L e TRAIL), quanto a sua capacidade de ativar o receptor da via de morte, no plasma de indivíduos chagásicos com insuficiência cardíaca tanto branda e grave quanto de indivíduos nãoinfectados correlacionando clinicamente (ecocardiografia e eletrocardiografia) em relação aos dados bioquímicos. A concentração de TNF-alfa e TRAIL foi claramente aumentada somente nos indivíduos com a forma grave de CCC enquanto Fas ligante/CD95L foi detectada em todos os indivíduos com doença de Chagas. Todos os ligantes de TNF apresentaram baixas concentrações no plasma de indivíduos de voluntários não-infectados pelo parasito Trypanosoma cruzi. Uma boa correlação foi encontrada entre TNF-alpha (r=0.85 e r=0.68) ou TRAIL (r=0.68 e r=0.60) e os parâmetros funcionais ventriculares como fração de ejeção ventricular esquerda (LVEF) e diâmetro diastólico ventricular esquerdo (LVDD), respectivamente. Apesar da sensibilidade destes, Fas ligante/CD95L apresentou uma fraca correlação (r=0.50) para ambos LVEF e LVDD. Curiosamente, os ligantes da superfamília do TNF foram mais correlacionados somente com os parâmetros funcionais, mas não com eventos arrítmicos. Mais estudos são necessários ainda, mas juntos nossos dados sugerem uma participação de eventos apoptóticos em distúrbios funcionais do ventrículo esquerdo durante a CCC e uma intervenção precoce na via de apoptose poderá preservar alguns parâmetros funcionais cardíacos durante a doença de Chagas crônica.Apoptosis of cardiomyocytes has been reported to be involved in the pathogenesis of human chronic Chagas cardiomyopathy (CCC). To determine wheter the apoptotic machinery is correlated with the ventricular arrhytmogenic and functional disturbances in CCC, we examined the TNF superfamily ligands (TNF-alpha, Fas ligand/CD95L and TRAIL), by their capacity to activate death receptor pathway, in plasma from chagasic individual with mild and severe heart failure as well as in noninfected subjects correlating clinical (echocardiography and eletrocardiography) and biochemical data. The concentration of TNF-alpha and TRAIL was clearly augmented only in individuals with servere form of CCC while Fas ligand/CD95L was more sensitive to be detected in all individuals with Chagas disease. All TNF ligands presented low concentrations in plasma from voluntaries non-infected by the parasite Trypanosoma cruzi. A good correlation was found among TNF-alpha (r=0.85 and r=0.68) or TRAIL (r=0.68 and r=0.60) and the functional ventricle parameters like left ventricular ejection fraction (LVEF) and left ventricle diastolic diameter (LVDD), respectivly. Notwithstanding its sensitivity, Fas ligand/CD95L presented a weak correlation (r=0.50) for both LVEF and LVDD. Interestingly, TNF superfamily ligands were more correlated only with functional parameters, but not with arrhytmogenic events. More studies are still necessary, but together our data suggest a participation of apoptotic events in left ventricle funcional disturbances during CCC and an early intervention in the apoptosis pathway could preserve some cardiac functional parameters during chronic Chagas disease

Níveis plasmáticos de TNF-alfa, TRAIL e FasLigante/CD95L correlacionados com a disfunção ventricular em pacientes com cardiopatia chagásica crônica Ouro Preto 2008.

Apoptose de cardiomiócitos tem sido reportado estar envolvido na patogênese da cardiomiopatia chagásica crônica (CCC). Para determinar se a maquinaria apoptótica está correlacionada aos distúrbios arrítmicos ventriculares e funcionais na CCC, foi examinado os ligantes da superfamília do TNF (TNF-alfa, Fasligante/CD95L e TRAIL), quanto a sua capacidade de ativar o receptor da via de morte, no plasma de indivíduos chagásicos com insuficiência cardíaca tanto branda e grave quanto de indivíduos nãoinfectados correlacionando clinicamente (ecocardiografia e eletrocardiografia) em relação aos dados bioquímicos. A concentração de TNF-alfa e TRAIL foi claramente aumentada somente nos indivíduos com a forma grave de CCC enquanto Fas ligante/CD95L foi detectada em todos os indivíduos com doença de Chagas. Todos os ligantes de TNF apresentaram baixas concentrações no plasma de indivíduos de voluntários não-infectados pelo parasito Trypanosoma cruzi. Uma boa correlação foi encontrada entre TNF-alpha (r=0.85 e r=0.68) ou TRAIL (r=0.68 e r=0.60) e os parâmetros funcionais ventriculares como fração de ejeção ventricular esquerda (LVEF) e diâmetro diastólico ventricular esquerdo (LVDD), respectivamente. Apesar da sensibilidade destes, Fas ligante/CD95L apresentou uma fraca correlação (r=0.50) para ambos LVEF e LVDD. Curiosamente, os ligantes da superfamília do TNF foram mais correlacionados somente com os parâmetros funcionais, mas não com eventos arrítmicos. Mais estudos são necessários ainda, mas juntos nossos dados sugerem uma participação de eventos apoptóticos em distúrbios funcionais do ventrículo esquerdo durante a CCC e uma intervenção precoce na via de apoptose poderá preservar alguns parâmetros funcionais cardíacos durante a doença de Chagas crônica.Apoptosis of cardiomyocytes has been reported to be involved in the pathogenesis of human chronic Chagas cardiomyopathy (CCC). To determine wheter the apoptotic machinery is correlated with the ventricular arrhytmogenic and functional disturbances in CCC, we examined the TNF superfamily ligands (TNF-alpha, Fas ligand/CD95L and TRAIL), by their capacity to activate death receptor pathway, in plasma from chagasic individual with mild and severe heart failure as well as in noninfected subjects correlating clinical (echocardiography and eletrocardiography) and biochemical data. The concentration of TNF-alpha and TRAIL was clearly augmented only in individuals with servere form of CCC while Fas ligand/CD95L was more sensitive to be detected in all individuals with Chagas disease. All TNF ligands presented low concentrations in plasma from voluntaries non-infected by the parasite Trypanosoma cruzi. A good correlation was found among TNF-alpha (r=0.85 and r=0.68) or TRAIL (r=0.68 and r=0.60) and the functional ventricle parameters like left ventricular ejection fraction (LVEF) and left ventricle diastolic diameter (LVDD), respectivly. Notwithstanding its sensitivity, Fas ligand/CD95L presented a weak correlation (r=0.50) for both LVEF and LVDD. Interestingly, TNF superfamily ligands were more correlated only with functional parameters, but not with arrhytmogenic events. More studies are still necessary, but together our data suggest a participation of apoptotic events in left ventricle funcional disturbances during CCC and an early intervention in the apoptosis pathway could preserve some cardiac functional parameters during chronic Chagas disease

Plasma concentrations of tumour necrosis factor-alpha, tumour necrosis factor-related apoptosis-inducing ligand, and FasLigand/CD95L in patients with Chagas cardiomyopathy correlate with left ventricular dysfunction.

Cardiomyocyte apoptosis is reported to be involved in the pathogenesis of human chronic Chagas cardiomyopathy (CCC). Members of the tumour necrosis factor (TNF) superfamily (TNF-a, FasLigand/CD95L, and TNF-related apoptosis-inducing ligand) are known to activate the death receptor pathway. We therefore investigated whether levels of TNF-a, FasLigand/CD95L, and TRAIL correlated with changes in heart function of patients with Chagas disease (n ? 31). Concentrations of TNF-a and TRAIL were clearly augmented in individuals with severe form CCC (n ? 16). Levels of FasLigand/CD95L were greater in chagasic patients than in non-infected individuals (n ? 15) but did not differentiate between clinical forms of Chagas disease. There was a good correlation between TNF-a (r ? 0.85 and r ? 0.68, P , 0.0001) or TRAIL (r ? 0.68 and r ? 0.60, P , 0.001) and left ventricular ejection fraction (LVEF) and left ventricular diastolic diameter (LVDD), respectively. In addition, TNF-a (r ? 0.57, P ? 0.0001), TRAIL (r ? 0.56, P ? 0.001), and FasLigand/CD95L (r ? 0.51, P ? 0.001) showed a good correlation with brain natriuretic peptide, a well-known parameter of ventricular dysfunction in CCC. There was a weak correlation between levels of FasLigand/CD95L (r ? 0.50, P , 0.004) and both LVEF and LVDD. There was no correlation between levels of TNF superfamily ligands and chronotropic incompetence, maximal heart rate, or number of ventricular premature beats in 24 h. Plasma levels of TNF superfamily ligands are elevated in patients with functional but not arrhythmogenic disturbances, and these death receptor ligands may be potential markers of ventricular dysfunction in CCC

Neotropical freshwater fisheries : A dataset of occurrence and abundance of freshwater fishes in the Neotropics

The Neotropical region hosts 4225 freshwater fish species, ranking first among the world's most diverse regions for freshwater fishes. Our NEOTROPICAL FRESHWATER FISHES data set is the first to produce a large-scale Neotropical freshwater fish inventory, covering the entire Neotropical region from Mexico and the Caribbean in the north to the southern limits in Argentina, Paraguay, Chile, and Uruguay. We compiled 185,787 distribution records, with unique georeferenced coordinates, for the 4225 species, represented by occurrence and abundance data. The number of species for the most numerous orders are as follows: Characiformes (1289), Siluriformes (1384), Cichliformes (354), Cyprinodontiformes (245), and Gymnotiformes (135). The most recorded species was the characid Astyanax fasciatus (4696 records). We registered 116,802 distribution records for native species, compared to 1802 distribution records for nonnative species. The main aim of the NEOTROPICAL FRESHWATER FISHES data set was to make these occurrence and abundance data accessible for international researchers to develop ecological and macroecological studies, from local to regional scales, with focal fish species, families, or orders. We anticipate that the NEOTROPICAL FRESHWATER FISHES data set will be valuable for studies on a wide range of ecological processes, such as trophic cascades, fishery pressure, the effects of habitat loss and fragmentation, and the impacts of species invasion and climate change. There are no copyright restrictions on the data, and please cite this data paper when using the data in publications