157 research outputs found
Purchases, Penalties, and Power: The Relationship between Earnings and Housework
Studies examining the association of housework with earnings have not tested for causal directionality despite competing theories about causal ordering. Autonomy theory, and the relative resources, gender display, and gender deviance neutralization hypotheses suggest personal and relative earnings affect time in housework while human capital theory implies the opposite. Using data from N = 3,719 continuously married couples in Waves 1 and 2 of the National Survey of Families and Households and structural equation models, we find that wives\u27 personal earnings and housework are reciprocally related. Her earnings have a stronger effect on housework than vice versa. For husbands, time in routine housework affects earnings only. We find little evidence that relative earnings affect husbands’ or wives’ housework time, rather we identify a significant effect of housework on one’s share of couple’s earnings. The results provide support for autonomy theory for wives and a human capital perspective for both spouses
The Effect of a Novel Proactive First Day Prescription Counseling Program on Adherence to Select Cardiovascular Medications
Objective: To determine the impact of a proactive first day prescription counseling program on medication adherence to new cardiovascular maintenance medicationsDesign: Cross-sectional studySetting: Regional chain community pharmacy in Pittsburgh, Pennsylvania; August 2009 through November 2010Patients: Data was collected from all patients aged 18-89 presenting with a new or transferred prescription or change in dosage within the study dates at four study locationsInterventions: Patients presenting with a new or transferred prescription or change in dosage were identified to receive pharmacist or student pharmacist counseling. Data from the counseling session was tracked weekly to determine if the program increased adherence to statins, ACEIs and/or ARBs.Main Outcome Measured: Adherence to statins, ACEIs and/or ARBs was determined by differences in proportion of days covered (PDC) at six months and medication persistence to therapy.Results: Analysis was conducted using IDNA sm software. Results of the 6,916 prescriptions included in the study revealed that persistence rates for statins was 32.5% (intervention) and 34.2% (control) (p<0.001); ACEI/ARBs persistence was 37.3% (intervention) and 43.2% (control) (p<0.001). PDC was nonsignificant with respect to statins; 43.2% (intervention) and 45.1% (control); and 50.2% (intervention) and 57.1% (control) (p<0.001) for the ACEI/ARBs.Conclusion: Results from this study showed no improvement in adherence of statins or ACEIs/ARBs with the D1TC program versus control pharmacies, although several important limitations were identified. It is clear that a variety of methods and programs are needed to consistently improve adherence to maintenance medications
The transcriptomic evolution of mammalian pregnancy:gene expression innovations in endometrial stromal fibroblasts
The endometrial stromal fibroblast (ESF) is a cell type present in the uterine lining of therian mammals. In the stem lineage of eutherian mammals, ESF acquired the ability to differentiate into decidual cells in order to allow embryo implantation. We call the latter cell type “neo-ESF” in contrast to “paleo-ESF” which is homologous to eutherian ESF but is not able to decidualize. In this study, we compare the transcriptomes of ESF from six therian species: Opossum (Monodelphis domestica; paleo-ESF), mink, rat, rabbit, human (all neo-ESF), and cow (secondarily nondecidualizing neo-ESF). We find evidence for strong stabilizing selection on transcriptome composition suggesting that the expression of approximately 5,600 genes is maintained by natural selection. The evolution of neo-ESF from paleo-ESF involved the following gene expression changes: Loss of expression of genes related to inflammation and immune response, lower expression of genes opposing tissue invasion, increased markers for proliferation as well as the recruitment of FOXM1, a key gene transiently expressed during decidualization. Signaling pathways also evolve rapidly and continue to evolve within eutherian lineages. In the bovine lineage, where invasiveness and decidualization were secondarily lost, we see a re-expression of genes found in opossum, most prominently WISP2, and a loss of gene expression related to angiogenesis. The data from this and previous studies support a scenario, where the proinflammatory paleo-ESF was reprogrammed to express anti-inflammatory genes in response to the inflammatory stimulus coming from the implanting conceptus and thus paving the way for extended, trans-cyclic gestation
Detection of Crab Giant Pulses Using the Mileura Widefield Array Low Frequency Demonstrator Field Prototype System
We report on the detection of giant pulses from the Crab Nebula pulsar at a
frequency of 200 MHz using the field deployment system designed for the Mileura
Widefield Array's Low Frequency Demonstrator (MWA-LFD). Our observations are
among the first high-quality detections at such low frequencies. The measured
pulse shapes are deconvolved for interstellar pulse broadening, yielding a
pulse-broadening time of 670100 s, and the implied strength of
scattering (scattering measure) is the lowest that is estimated towards the
Crab nebula from observations made so far. The sensitivity of the system is
largely dictated by the sky background, and our simple equipment is capable of
detecting pulses that are brighter than 9 kJy in amplitude. The brightest
giant pulse detected in our data has a peak amplitude of 50 kJy, and the
implied brightness temperature is K. We discuss the giant pulse
detection prospects with the full MWA-LFD system. With a sensitivity over two
orders of magnitude larger than the prototype equipment, the full system will
be capable of detecting such bright giant pulses out to a wide range of
Galactic distances; from 8 to 30 kpc depending on the frequency.
The MWA-LFD will thus be a highly promising instrument for the studies of giant
pulses and other fast radio transients at low frequencies.Comment: 10 pages, 6 figures, Accepted for publication in the Astrophysical
Journa
The Murchison Widefield Array: Design Overview
The Murchison Widefield Array (MWA) is a dipole-based aperture array
synthesis telescope designed to operate in the 80-300 MHz frequency range. It
is capable of a wide range of science investigations, but is initially focused
on three key science projects. These are detection and characterization of
3-dimensional brightness temperature fluctuations in the 21cm line of neutral
hydrogen during the Epoch of Reionization (EoR) at redshifts from 6 to 10,
solar imaging and remote sensing of the inner heliosphere via propagation
effects on signals from distant background sources,and high-sensitivity
exploration of the variable radio sky. The array design features 8192
dual-polarization broad-band active dipoles, arranged into 512 tiles comprising
16 dipoles each. The tiles are quasi-randomly distributed over an aperture
1.5km in diameter, with a small number of outliers extending to 3km. All
tile-tile baselines are correlated in custom FPGA-based hardware, yielding a
Nyquist-sampled instantaneous monochromatic uv coverage and unprecedented point
spread function (PSF) quality. The correlated data are calibrated in real time
using novel position-dependent self-calibration algorithms. The array is
located in the Murchison region of outback Western Australia. This region is
characterized by extremely low population density and a superbly radio-quiet
environment,allowing full exploitation of the instrumental capabilities.Comment: 9 pages, 5 figures, 1 table. Accepted for publication in Proceedings
of the IEE
Species and population specific gene expression in blood transcriptomes of marine turtles
Background: Transcriptomic data has demonstrated utility to advance the study of physiological diversity and organisms’ responses to environmental stressors. However, a lack of genomic resources and challenges associated with collecting high-quality RNA can limit its application for many wild populations. Minimally invasive blood sampling combined with de novo transcriptomic approaches has great potential to alleviate these barriers. Here, we advance these goals for marine turtles by generating high quality de novo blood transcriptome assemblies to characterize functional diversity and compare global transcriptional profiles between tissues, species, and foraging aggregations. Results: We generated high quality blood transcriptome assemblies for hawksbill (Eretmochelys imbricata), loggerhead (Caretta caretta), green (Chelonia mydas), and leatherback (Dermochelys coriacea) turtles. The functional diversity in assembled blood transcriptomes was comparable to those from more traditionally sampled tissues. A total of 31.3% of orthogroups identified were present in all four species, representing a core set of conserved genes expressed in blood and shared across marine turtle species. We observed strong species-specific expression of these genes, as well as distinct transcriptomic profiles between green turtle foraging aggregations that inhabit areas of greater or lesser anthropogenic disturbance. Conclusions: Obtaining global gene expression data through non-lethal, minimally invasive sampling can greatly expand the applications of RNA-sequencing in protected long-lived species such as marine turtles. The distinct differences in gene expression signatures between species and foraging aggregations provide insight into the functional genomics underlying the diversity in this ancient vertebrate lineage. The transcriptomic resources generated here can be used in further studies examining the evolutionary ecology and anthropogenic impacts on marine turtles
Field Deployment of Prototype Antenna Tiles for the Mileura Widefield Array--Low Frequency Demonstrator
Experiments were performed with prototype antenna tiles for the Mileura
Widefield Array--Low Frequency Demonstrator (MWA-LFD) to better understand the
widefield, wideband properties of their design and to characterize the radio
frequency interference (RFI) between 80 and 300 MHz at the site in Western
Australia. Observations acquired during the six month deployment confirmed the
predicted sensitivity of the antennas, sky-noise dominated system temperatures,
and phase-coherent interferometric measurements. The radio spectrum is
remarkably free of strong terrestrial signals, with the exception of two narrow
frequency bands allocated to satellite downlinks and rare bursts due to
ground-based transmissions being scattered from aircraft and meteor trails.
Results indicate the potential of the MWA-LFD to make significant achievements
in its three key science objectives: epoch of reionziation science,
heliospheric science, and radio transient detection.Comment: Accepted by AJ. 17 pages with figure
BAC-Based Sequencing of Behaviorally-Relevant Genes in the Prairie Vole
The prairie vole (Microtus ochrogaster) is an important model organism for the study of social behavior, yet our ability to correlate genes and behavior in this species has been limited due to a lack of genetic and genomic resources. Here we report the BAC-based targeted sequencing of behaviorally-relevant genes and flanking regions in the prairie vole. A total of 6.4 Mb of non-redundant or haplotype-specific sequence assemblies were generated that span the partial or complete sequence of 21 behaviorally-relevant genes as well as an additional 55 flanking genes. Estimates of nucleotide diversity from 13 loci based on alignments of 1.7 Mb of haplotype-specific assemblies revealed an average pair-wise heterozygosity (8.4×10−3). Comparative analyses of the prairie vole proteins encoded by the behaviorally-relevant genes identified >100 substitutions specific to the prairie vole lineage. Finally, our sequencing data indicate that a duplication of the prairie vole AVPR1A locus likely originated from a recent segmental duplication spanning a minimum of 105 kb. In summary, the results of our study provide the genomic resources necessary for the molecular and genetic characterization of a high-priority set of candidate genes for regulating social behavior in the prairie vole
Ordering of mutations in preinvasive disease stages of esophageal carcinogenesis.
Cancer genome sequencing studies have identified numerous driver genes, but the relative timing of mutations in carcinogenesis remains unclear. The gradual progression from premalignant Barrett's esophagus to esophageal adenocarcinoma (EAC) provides an ideal model to study the ordering of somatic mutations. We identified recurrently mutated genes and assessed clonal structure using whole-genome sequencing and amplicon resequencing of 112 EACs. We next screened a cohort of 109 biopsies from 2 key transition points in the development of malignancy: benign metaplastic never-dysplastic Barrett's esophagus (NDBE; n=66) and high-grade dysplasia (HGD; n=43). Unexpectedly, the majority of recurrently mutated genes in EAC were also mutated in NDBE. Only TP53 and SMAD4 mutations occurred in a stage-specific manner, confined to HGD and EAC, respectively. Finally, we applied this knowledge to identify high-risk Barrett's esophagus in a new non-endoscopic test. In conclusion, mutations in EAC driver genes generally occur exceptionally early in disease development with profound implications for diagnostic and therapeutic strategies
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