Identification of a novel retroviral gene unique to human immunodeficiency virus type 2 and simian immunodeficiency virus SIVMAC

Human and simian immunodeficiency-associated retroviruses are extraordinarily complex, containing at least five genes, tat, art, sor, R, and 3' orf, in addition to the structural genes gag, pol, and env. Recently, nucleotide sequence analysis of human immunodeficiency virus type 2 (HIV-2) and simian immunodeficiency virus SIVMAC revealed the existence of still another open reading frame, termed X, which is highly conserved between these two viruses but absent from HIV-1. In this report, we demonstrate for the first time that the X open reading frame represents a functional retroviral gene in both HIV-2 and SIVMAC and that it encodes a virion-associated protein of 14 and 12 kilodaltons, respectively. We also describe the production of recombinant TrpE/X fusion proteins in Escherichia coli and show that sera from some HIV-2-infected individuals specifically recognize these proteins

Terahertz imaging and spectroscopy of large-area single-layer graphene

We demonstrate terahertz (THz) imaging and spectroscopy of a 15x15-mm^2 single-layer graphene film on Si using broadband THz pulses. The THz images clearly map out the THz carrier dynamics of the graphene-on-Si sample, allowing us to measure sheet conductivity with sub-mm resolution without fabricating electrodes. The THz carrier dynamics are dominated by intraband transitions and the THz-induced electron motion is characterized by a flat spectral response. A theoretical analysis based on the Fresnel coefficients for a metallic thin film shows that the local sheet conductivity varies across the sample from {\sigma}s = 1.7x10^-3 to 2.4x10^-3 {\Omega}^-1 (sheet resistance, {\rho}s = 420 - 590 {\Omega}/sq).Comment: 6 pages, 5 figure

Cold gas outflows from the Small Magellanic Cloud traced with ASKAP

Feedback from massive stars plays a critical role in the evolution of the Universe by driving powerful outflows from galaxies that enrich the intergalactic medium and regulate star formation. An important source of outflows may be the most numerous galaxies in the Universe: dwarf galaxies. With small gravitational potential wells, these galaxies easily lose their star-forming material in the presence of intense stellar feedback. Here, we show that the nearby dwarf galaxy, the Small Magellanic Cloud (SMC), has atomic hydrogen outflows extending at least 2 kiloparsecs (kpc) from the star-forming bar of the galaxy. The outflows are cold, $T<400~{\rm K}$, and may have formed during a period of active star formation $25 - 60$ million years (Myr) ago. The total mass of atomic gas in the outflow is $\sim 10^7$ solar masses, ${\rm M_{\odot}}$, or $\sim 3$% of the total atomic gas of the galaxy. The inferred mass flux in atomic gas alone, $\dot{M}_{HI}\sim 0.2 - 1.0~{\rm M_{\odot}~yr^{-1}}$, is up to an order of magnitude greater than the star formation rate. We suggest that most of the observed outflow will be stripped from the SMC through its interaction with its companion, the Large Magellanic Cloud (LMC), and the Milky Way, feeding the Magellanic Stream of hydrogen encircling the Milky Way.Comment: Published in Nature Astronomy, 29 October 2018, http://dx.doi.org/10.1038/s41550-018-0608-

Molecular dynamics simulations of oxide memristors: thermal effects

We have extended our recent molecular-dynamic simulations of memristors to include the effect of thermal inhomogeneities on mobile ionic species appearing during operation of the device. Simulations show a competition between an attractive short-ranged interaction between oxygen vacancies and an enhanced local temperature in creating/destroying the conducting oxygen channels. Such a competition would strongly affect the performance of the memristive devices.Comment: submit/0169777; 6 pages, 4 figure

Ultraviolet Emission Line Ratios of Cataclysmic Variables

We present a statistical analysis of the ultraviolet emission lines of cataclysmic variables (CVs) based on $\approx 430$ ultraviolet spectra of 20 sources extracted from the International Ultraviolet Explorer Uniform Low Dispersion Archive. These spectra are used to measure the emission line fluxes of N V, Si IV, C IV, and He II and to construct diagnostic flux ratio diagrams. We investigate the flux ratio parameter space populated by individual CVs and by various CV subclasses (e.g., AM Her stars, DQ Her stars, dwarf novae, nova-like variables). For most systems, these ratios are clustered within a range of $\sim 1$ decade for log Si IV/C IV $\approx -0.5$ and log He II/C IV $\approx -1.0$ and $\sim 1.5$ decades for log N V/C IV $\approx -0.25$. These ratios are compared to photoionization and collisional ionization models to constrain the excitation mechanism and the physical conditions of the line-emitting gas. We find that the collisional models do the poorest job of reproducing the data. The photoionization models reproduce the Si IV/C IV line ratios for some shapes of the ionizing spectrum, but the predicted N V/C IV line ratios are simultaneously too low by typically $\sim 0.5$ decades. Worse, for no parameters are any of the models able to reproduce the observed He II/C IV line ratios; this ratio is far too small in the collisional and scattering models and too large by typically $\sim 0.5$ decades in the photoionization models.Comment: LaTeX format, uses aaspp4.sty, 28 pages, 11 Postscript figures, accepted for publication in The Astrophysical Journal 10/16/9

γδ T Cells Are Reduced and Rendered Unresponsive by Hyperglycemia and Chronic TNFα in Mouse Models of Obesity and Metabolic Disease

Epithelial cells provide an initial line of defense against damage and pathogens in barrier tissues such as the skin; however this balance is disrupted in obesity and metabolic disease. Skin γδ T cells recognize epithelial damage, and release cytokines and growth factors that facilitate wound repair. We report here that hyperglycemia results in impaired skin γδ T cell proliferation due to altered STAT5 signaling, ultimately resulting in half the number of γδ T cells populating the epidermis. Skin γδ T cells that overcome this hyperglycemic state are unresponsive to epithelial cell damage due to chronic inflammatory mediators, including TNFα. Cytokine and growth factor production at the site of tissue damage was partially restored by administering neutralizing TNFα antibodies in vivo. Thus, metabolic disease negatively impacts homeostasis and functionality of skin γδ T cells, rendering host defense mechanisms vulnerable to injury and infection