Prototype of a new Engineering Masters project model: Working with marketing and software faculties to commercially kickstart university research

We describe a Master of Engineering (500-level) project modelled on the real-world arrangement where engineers work with marketing and software groups to prepare a product for commercialisation. A 4-member software team to develop and test embedded firmware and support applications on a mobile platform was provided through a final-year undergraduate software-engineering project course based outside the engineering school, in a separate faculty. A marketing team consisting of interns prepared logos, product names, and advertising materials, with input from a creative 200-level class. This team also considered possible exit strategies based on analysis of the market size and activity. This marketing effort was organised through the management communications group in the management school. The masters student acts as project manager and it is their remit to guide the product towards release on the crowd-sourced venture-capital site kickstarter.com. A small but original product idea is required to provide a viable vehicle for the project. Financial commitment to manufacture, even on a small scale, represents a novel outcome for a university project

Obstacles to option setting: Initial results with a heuristic walkthrough method

Abstract. This short paper is the first step in a line of research that aims to deepen understanding of the difficulties that users often have with option setting interfaces: those parts of a system that allow the user to set parameters that influence the system's behavior and appearance. On the basis of a theoretical distinction of three things that users may fail to understand about a given option, we introduce a simple variant of the heuristic walkthrough method that helps evaluators to uncover likely obstacles. We give a quantitative and qualitative overview of the obstacles found through the application of this heuristic walkthrough to parts of four popular applications. Option Setting Interfaces as the Stepchild of Interface Design With regard to most aspects of mainstream graphical user interfaces and web-based systems, we have an abundance of design guidelines, along with convenient methods for inspection-based evaluation (e.g., heuristic evaluation and cognitive walkthrough). But one common part of these systems tends to remain in the shadows: those screens full of "options" (or "preferences") that each user can set to make the system work in a way that he or she will find satisfying in the long run. Everyday experience and conventional wisdom suggest that many users find it unduly difficult and/or timeconsuming to figure out suitable option settings for themselves; and several studies conducted in the 1990s (e.g., An exception is found in research on privacy and security options and preferences (see, e.g.

Uric acid and thiocyanate as competing substrates of lactoperoxidase

The physiological function of urate is poorly understood. It may act as a danger signal, an antioxidant, or a substrate for heme peroxidases. Whether it reacts sufficiently rapidly with lactoperoxidase (LPO) to act as a physiological substrate remains unknown. LPO is a mammalian peroxidase that plays a key role in the innate immune defense by oxidizing thiocyanate to the bactericidal and fungicidal agent hypothiocyanite. We now demonstrate that urate is a good substrate for bovine LPO

In vitro Di-ubiquitin Formation Assay and E3 Cooperation Assay

Ubiquitination is a post-translational modification conserved across eukaryotic species. It contributes to a variety of regulatory pathways, including proteasomal degradation, DNA repair, and cellular differentiation. The ubiquitination of substrate proteins typically requires three ubiquitination enzymes: a ubiquitin-activating E1, a ubiquitin-conjugating E2, and an E3 ubiquitin ligase. Cooperation between E2s and E3s is required for substrate ubiquitination, but some ubiquitin-conjugating E2s are also able to catalyze by themselves the formation of free di-ubiquitin, independently or in cooperation with a ubiquitin E2 variant. Here, we describe a method for assessing (i) di-ubiquitin formation by an E1 together with an E2 and an E2 variant, and (ii) the cooperation of an E3 with an E1 and E2 (with or without the E2 variant). Reaction products are assessed using western blotting with one of two antibodies: the first detects all ubiquitin conjugates, while the second specifically recognizes K63-linked ubiquitin. This allows unambiguous identification of ubiquitinated species and assessment of whether K63 linkages are present. We have developed these methods for studying ubiquitination proteins of Leishmania mexicana, specifically the activities of the E2, UBC2, and the ubiquitin E2 variant UEV1, but we anticipate the assays to be applicable to other ubiquitination systems with UBC2/UEV1 orthologues