Mapping neighborhood scale survey responses with uncertainty metrics

This paper presents a methodology of mapping population-centric social, infrastructural, and environmental metrics at neighborhood scale. This methodology extends traditional survey analysis methods to create cartographic products useful in agent-based modeling and geographic information analysis. It utilizes and synthesizes survey microdata, sub-upazila attributes, land use information, and ground truth locations of attributes to create neighborhood scale multi-attribute maps. Monte Carlo methods are employed to combine any number of survey responses to stochastically weight survey cases and to simulate survey cases\u27 locations in a study area. Through such Monte Carlo methods, known errors from each of the input sources can be retained. By keeping individual survey cases as the atomic unit of data representation, this methodology ensures that important covariates are retained and that ecological inference fallacy is eliminated. These techniques are demonstrated with a case study from the Chittagong Division in Bangladesh. The results provide a population-centric understanding of many social, infrastructural, and environmental metrics desired in humanitarian aid and disaster relief planning and operations wherever long term familiarity is lacking. Of critical importance is that the resulting products have easy to use explicit representation of the errors and uncertainties of each of the input sources via the automatically generated summary statistics created at the application\u27s geographic scale

Trends in E-Cigarette and Tobacco Cigarette Purchasing Behaviors by Youth in the United States, Canada, and England, 2017–2022

Objectives: This paper describes trends in youth e-cigarette (EC) and tobacco cigarette (TC) purchasing behaviors in Canada, England, and the United States (US) in relationship to changing minimum legal age (MLA) laws.Methods: Data are from eight cross-sectional online surveys among national samples of 16- to 19-year-olds in Canada, England, and the US conducted from 2017 to 2022 (N = 104,467). Average wave percentage change in EC and TC purchasing prevalence and purchase locations were estimated using Joinpoint regressions.Results: EC purchasing increased between 2017 and 2022, although the pattern of change differed by country. EC purchasing plateaued in 2019 for the US and in 2020 for Canada, while increasing through 2022 for England. TC purchasing declined sharply in the US, with purchasing from traditional retail locations declining, while purchasing from social sources increased. Vape shops were the most common location for EC purchasing, although declining in England and the US.Conclusion: Trends in EC and TC purchasing trends in the US are consistent with the expected impact of the federal MLA law increasing the legal age to 21 years in December 2019

Timeless Links Replication Termination to Mitotic Kinase Activation

The mechanisms that coordinate the termination of DNA replication with progression through mitosis are not completely understood. The human Timeless protein (Tim) associates with S phase replication checkpoint proteins Claspin and Tipin, and plays an important role in maintaining replication fork stability at physical barriers, like centromeres, telomeres and ribosomal DNA repeats, as well as at termination sites. We show here that human Tim can be isolated in a complex with mitotic entry kinases CDK1, Auroras A and B, and Polo-like kinase (Plk1). Plk1 bound Tim directly and colocalized with Tim at a subset of mitotic structures in M phase. Tim depletion caused multiple mitotic defects, including the loss of sister-chromatid cohesion, loss of mitotic spindle architecture, and a failure to exit mitosis. Tim depletion caused a delay in mitotic kinase activity in vivo and in vitro, as well as a reduction in global histone H3 S10 phosphorylation during G2/M phase. Tim was also required for the recruitment of Plk1 to centromeric DNA and formation of catenated DNA structures at human centromere alpha satellite repeats. Taken together, these findings suggest that Tim coordinates mitotic kinase activation with termination of DNA replication

Trends in e-cigarette brands, devices and the nicotine profile of products used by youth in England, Canada and the USA:2017-2019

Background: The e-cigarette market has rapidly evolved, with a shift towards higher nicotine concentration and salt-based products, such as JUUL; however, the implications for youth vaping remain unclear. Methods: Repeat cross-sectional online surveys were conducted in 2017, 2018 and 2019, with national samples of youth aged 16–19 years recruited from commercial panels in Canada (n=12 018), England (n=11 362) and the USA (n=12 110). Regression models examined differences between countries and over time in the types of e-cigarette products used (design and nicotine content), reasons for using brands and differences in patterns of use, sociodemographics and dependence symptoms by brand/nicotine content. Results: In 2019, the use of pod- or cartridge-style e-cigarettes was greater in Canada and the USA than England, with Smok and JUUL the leading brands in all countries. In 2019, youth vapers in England were less likely to report using e-cigarettes with ≥2% nicotine (12.8%) compared with Canada (40.5%; adjusted OR (AOR)=4.96; 95% CI 3.51 to 7.01) and the USA (37.0%; AOR=3.99, 95% CI 2.79 to 5.71) and less likely to report using nicotine salt-based products (12.3%) compared with Canada (27.1%; AOR=2.77, 95% CI 1.93 to 3.99) and the USA (21.9%; AOR=2.00, 95% CI 1.36 to 2.95). In 2019, self-reported use of products with higher nicotine concentration was associated with significantly greater frequency of vaping, urges to vape and perceived vaping addiction (p<0.05 for all). Conclusions The use of high-nicotine salt-based products is associated with greater symptoms of dependence, including JUUL and other higher-nicotine brands. Greater use of high-nicotine salt-based products may account for recent increases in the frequency of vaping among youth in Canada and the USA

Exposure to negative news stories and harm perceptions of e-cigarettes before and after the US outbreak of vaping-associated lung injury (EVALI) among youth in the US, Canada, and England

Includes pre-registration of analyses and code (STATA). Publication available at https://academic.oup.com/ntr/article/24/9/1386/656288

Exposure to Negative News Stories About Vaping, and Harm Perceptions of Vaping, Among Youth in England, Canada, and the United States Before and After the Outbreak of E-cigarette or Vaping-Associated Lung Injury ('EVALI')

INTRODUCTION: Little is known about the international impact of E-cigarette or Vaping-Associated Lung Injury (‘EVALI’) on youth perceptions of vaping harms. METHODS: Repeat cross-sectional online surveys of youth aged 16–19 years in England, Canada, and the United States before (2017, 2018), during (2019 August/September), and after (2020 February/March, 2020 August) the ‘EVALI’ outbreak (N = 63380). Logistic regressions assessed trends, country differences, and associations between exposure to negative news stories about vaping and vaping harm perceptions. RESULTS: Exposure to negative news stories increased between 2017 and February–March 2020 in England (12.6% to 34.2%), Canada (16.7% to 56.9%), and the United States (18.0% to 64.6%), accelerating during (2019) and immediately after (February–March 2020) the outbreak (p < .001) before returning to 2019 levels by August 2020. Similarly, the accurate perception that vaping is less harmful than smoking declined between 2017 and February–March 2020 in England (77.3% to 62.2%), Canada (66.3% to 43.3%), and the United States (61.3% to 34.0%), again accelerating during and immediately after the outbreak (p < .001). The perception that vaping takes less than a year to harm users’ health and worry that vaping will damage health also doubled over this period (p ≤ .001). Time trends were most pronounced in the United States. Exposure to negative news stories predicted the perception that vaping takes less than a year to harm health (Adjusted Odds Ratio = 1.55, 1.48-1.61) and worry that vaping will damage health (Adjusted Odds Ratio = 1.32, 1.18-1.48). CONCLUSIONS: Between 2017 and February–March 2020, youth exposure to negative news stories, and perceptions of vaping harms, increased, and increases were exacerbated during and immediately after ‘EVALI’. Effects were seen in all countries but were most pronounced in the United States. IMPLICATIONS: This is the first study examining changes in exposure to news stories about vaping, and perceptions of vaping harms, among youth in England, Canada, and the United States before, during, and after ‘EVALI’. Between 2017 and February–March 2020, youth exposure to negative news stories, and perceptions of vaping harms, increased, and increases were exacerbated during and immediately after ‘EVALI’. By August 2020, exposure to negative news stories returned to 2019 levels, while perceptions of harm were sustained. Exposure to negative news stories also predicted two of the three harm perception measures. Overall, findings suggest that ‘EVALI’ may have exacerbated youth’s perceptions of vaping harms internationally

Mapping neighborhood scale survey responses with uncertainty metrics

This paper presents a methodology of mapping population-centric social, infrastructural, and environmental metrics at neighborhood scale. This methodology extends traditional survey analysis methods to create cartographic products useful in agent-based modeling and geographic information analysis. It utilizes and synthesizes survey microdata, sub-upazila attributes, land use information, and ground truth locations of attributes to create neighborhood scale multi-attribute maps. Monte Carlo methods are employed to combine any number of survey responses to stochastically weight survey cases and to simulate survey cases\u27 locations in a study area. Through such Monte Carlo methods, known errors from each of the input sources can be retained. By keeping individual survey cases as the atomic unit of data representation, this methodology ensures that important covariates are retained and that ecological inference fallacy is eliminated. These techniques are demonstrated with a case study from the Chittagong Division in Bangladesh. The results provide a population-centric understanding of many social, infrastructural, and environmental metrics desired in humanitarian aid and disaster relief planning and operations wherever long term familiarity is lacking. Of critical importance is that the resulting products have easy to use explicit representation of the errors and uncertainties of each of the input sources via the automatically generated summary statistics created at the application\u27s geographic scale