East Java Propolis Inhibits cytokine Pro-inflammatory in Odontoblast like cells Human Pulp

Inflammation in pulp tissue is caused by caries bacteria. Most bacteria found are Lactobacillus acidophilus . Propolis is a sticky resin material that is derived from the bees and the surrounding plants, which are reported to have several biological effects including anti-bacterial and anti-inflammatory. This study will prove the effectiveness of propolis against proinflammatory cytokines on odontoblast like cells in human dental pulp. This study was performed on cultured odontoblast like cells in pulp. Cell culture was derived from the pulp tissue of human M3 teeth that had been extracted. Odontoblast pulp culture was divided in 3 groups, group 1 was cultured cells with propolis; group 2, cultured cells induced with Lactobacillus acidophilus ; group 3, cultured cells induced with inactive Lactobacillus acidophilus and exposed to 3μg/ml propolis. A measurement of TNF-α danTGF-β1 expression was done using the immunocytochemical technique to determine the effectiveness of propolis extracts from East Java Indonesia against proinfalmmatory cytokines. Data were analyzed using Anova test (p= 0,05) . Propolis extract can inhibit the expression of TNFα and increase the expression of TGF-β1on odontoblast like cell in human dental pulp. Anti-inflammatory effects of east Java propolis extract are associated with cytokine modulatio

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The Effect of Propolis Supplementation to CD4/CD8 Ratio in HIV-Infected Patients Receiving ARV Therapy

Background: The HIV infection affects the immune system by decreasing CD4/CD8 ratios. Previous studies reported that the use of nutrient supplements in HIV patients was quite high. Propolis is one of nutrient supplements that are reported to be beneficial in the immune system but evidences of its clinical benefits are limited. The aimed of this study was to reveal the advantageous outcome of propolis supplementation in patients on antiretroviral regimen therapy. Methods: The research was conducted in a double blind randomized controlled trial, pre and posttest control group including the treatment group (antiretroviral regimen + propolis) and the control group (antiretroviral regimen + placebo). CD4/CD8 ratio was checked at baseline and 6 months after the trial, as well as their safety profile, any side effect was documented and treated properly. Results: Forty-three subjects managed to reach the end of the study, consisting of 19 subjects in the treatment group and 21 subjects in the control group. Duviral-Neviral is the most widely used ARV regimen (51.2%). After 6 months of treatment, there was an increase in the mean CD4/CD8 ratio in the propolis group, but it was not statistically significant (p = 0.22). Interestingly, the delta CD4/CD8 ratio between propolis and placebo was significantly different (0.06 vs -0.01; p = 0.02). Conclusion: There were non-life-threatening side effects that occur in the propolis group subjects which improve after supplementation was stopped. To conclude, the effect of propolis supplementation on increasing CD4/CD8 ratio in HIV patients who had received antiretroviral regimen therapy did not reach statistical significance

Ipteks bagi masyarakat (IbM) kelompok peternak lebah di Desa Tulusbesar Malang

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A Novel Conserved Isoform of the Ubiquitin Ligase UFD2a/UBE4B Is Expressed Exclusively in Mature Striated Muscle Cells

Yeast Ufd2p was the first identified E4 multiubiquitin chain assembly factor. Its vertebrate homologues later referred to as UFD2a, UBE4B or E4B were also shown to have E3 ubiquitin ligase activity. UFD2a function in the brain has been well established in vivo, and in vitro studies have shown that its activity is essential for proper condensation and segregation of chromosomes during mitosis. Here we show that 2 alternative splice forms of UFD2a, UFD2a-7 and -7/7a, are expressed sequentially during myoblast differentiation of C2C12 cell cultures and during cardiotoxin-induced regeneration of skeletal muscle in mice. UFD2a-7 contains an alternate exon 7, and UFD2a-7/7a, the larger of the 2 isoforms, contains an additional novel exon 7a. Analysis of protein or mRNA expression in mice and zebrafish revealed that a similar pattern of isoform switching occurs during developmental myogenesis of cardiac and skeletal muscle. In vertebrates (humans, rodents, zebrafish), UFD2a-7/7a is expressed only in mature striated muscle. This unique tissue specificity is further validated by the conserved presence of 2 muscle-specific splicing regulatory motifs located in the 3′ introns of exons 7 and 7a. UFD2a interacts with VCP/p97, an AAA-type ATPase implicated in processes whose functions appear to be regulated, in part, through their interaction with one or more of 15 previously identified cofactors. UFD2a-7/7a did not interact with VCP/p97 in yeast 2-hybrid experiments, which may allow the ATPase to bind cofactors that facilitate its muscle-specific functions. We conclude that the regulated expression of these UFD2a isoforms most likely imparts divergent functions that are important for myogenisis

Ipteks bagi masyarakat (IbM) kelompok peternak lebah di Desa Tulusbesar Malang

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The application of empty fruit bunch (EFB) fibers of oil palm for the development of light structure materials and products

We have been involved in developing the use of EFB fibers for several light structural products, such as speed bumps, parking stoppers, helmets, and horse shoes. This paper presents our current research work on parking bumper made of EFB fibers to be utilized for power generation. The products are designed and produced in full scale covering the whole width of vehicles passing over the bumpers. The ideas are how to reserve kinetic energy harvested from the vehicles to convert into electricity to be stored in a battery. To obtain a structural integrity-proof material, the speed bumps were subjected several tests, e.g. static and impact tests as well as live body test using the kinetic energy obtained from vehicles pass over the speed bumps. For this, a speed bump station parallel to street zebra-cross was prepared. It was obtained that A-5 concrete foam type is suitable for 3B class road. The structural response of speed bumps were checked using ANSYS 17.0

The application of empty fruit bunch (EFB) fibers of oil palm for the development of light structure materials and products

We have been involved in developing the use of EFB fibers for several light structural products, such as speed bumps, parking stoppers, helmets, and horse shoes. This paper presents our current research work on parking bumper made of EFB fibers to be utilized for power generation. The products are designed and produced in full scale covering the whole width of vehicles passing over the bumpers. The ideas are how to reserve kinetic energy harvested from the vehicles to convert into electricity to be stored in a battery. To obtain a structural integrity-proof material, the speed bumps were subjected several tests, e.g. static and impact tests as well as live body test using the kinetic energy obtained from vehicles pass over the speed bumps. For this, a speed bump station parallel to street zebra-cross was prepared. It was obtained that A-5 concrete foam type is suitable for 3B class road. The structural response of speed bumps were checked using ANSYS 17.0