Addicts Speak: An Exploratory Ethnographic Study of Opioid Addiction

This thesis explores the experiences of people in recovery from opioid addiction in order to better understand the many process of recovery. Employing both participant observation and focused life history interview, and utilizing a grounded theory approach to data analysis, this research emphasizes data-driven conclusions. The research provides numerous insights into the process of recovery from opioid addiction, as well as factors that help to facilitate and sustain the process, the role that services play, and how services can be developed to better meet the needs of those in recovery

Oddaljenost neba: Analiza Guodian Wu Xing-a

This paper argues that the Guodian Wu Xing consists of two interrelated sections that reflect its distinction between goodness (a characteristic of humans) and virtue (a trait of Heaven). Individually, each section emphasizes different aspects of self-cultivation. When read against one another, they articulate the text’s main argument that Heaven is a distant figure and that the sage, a figure who understands the Way of Heaven, is almost unreachable. As such, the text focuses on the gentleman, a figure who achieves virtue (defined as timeliness) within a dispositional context by emulating Heaven in accordance with the Way of the Gentleman.Ta članek zagovarja trditev, da je Guodian Wu Xing sestavljen iz dveh medsebojno povezanih delov, ki izražata razlikovanje med dobroto (značilnost ljudi) in vrlino (lastnost neba). Vsak del posamično poudarja različne aspekte samo-kultivacije. Ko ju beremo vzporedno, se nam jasno izoblikuje osnovna trditev besedila, namreč da je nebo oddaljena podoba in da je modrec kot osebek, ki razume pot neba, skoraj nedosegljiv. Besedilo se tako osredotoča na plemenitnika, osebo, ki dosega vrlino (definirano kot pravočasnost) znotraj razpoložljivega konteksta, posnemajoč nebo v skladu s potjo plemenitega

Selaginella and the Satyr: Euptychia westwoodi (Lepidoptera: Nymphalidae) Oviposition Preference and Larval Performance

Members of the plant genus Selaginella (de Beauvois 1805) have few known insect herbivores even though they are considered by some to be ‘living fossils’, with extant taxa virtually indistinguishable from 300 Mya fossils. Butterflies are well-known herbivores, and the satyrs are among the most speciose of them despite having radiated ∼35 Mya ago. Nearly all satyrs feed on grass or sedges, but members of the Neotropical genus Euptychia Hübner 1818 feed on Selaginella; little is known about the degree to which this butterfly favors this ancient plant over those that its close relatives utilize. To advance our knowledge of Euptychia natural history, we conducted a series of experiments to examine oviposition preference and growth rates across a series of potential host plants on a Euptychia westwoodi population in Costa Rica. We found that Euptychia westwoodi Butler 1867 exhibit a strong preference to oviposit on Selaginella eurynota over the sympatric Selaginella arthritica, though they perform equally well as larvae on both plants. We did not observe oviposition on a sympatric grass that is commonly consumed by close relatives of E. westwoodi, and when larvae were offered the grass they refused to eat. These results suggest thatE. westwoodi in Costa Rica exhibit a strong preference for Selaginella and may have lost the ability to feed on a locally abundant grass commonly used by other Satyrinae

Pancratistatin induces apoptosis in clinical leukemia samples with minimal effect on non-cancerous peripheral blood mononuclear cells

<p>Abstract</p> <p>Background</p> <p>Pancratistatin, a natural compound extracted from <it>Hymenocallis littoralis</it>, can selectively induce apoptosis in several cancer cell lines. In this <it>ex vivo </it>study, we evaluated the effect of pancratistatin on peripheral blood mononuclear cells obtained from 15 leukemia patients prior to clinical intervention of newly diagnosed patients, as well as others of different ages in relapse and at various disease progression states.</p> <p>Results</p> <p>Mononuclear cells from healthy volunteers and leukemia patients were exposed to 1 μM pancratistatin for up to 48 h. Irrespective of leukemia type, pancratistatin induced apoptosis in the leukemic samples, with minimal effects on non-cancerous peripheral blood mononuclear control cells.</p> <p>Conclusion</p> <p>Our results show that pancratistatin is an effective and selective anti-cancer agent with potential for advancement to clinical trials.</p

Variations in Recycle Solids Concentrations in an Activated Sludge Plant

Bioenvironmental Engineerin

Twin RNA Polymerase–associated Proteins Control Virulence Gene Expression in Francisella tularensis

The MglA protein is the only known regulator of virulence gene expression in Francisella tularensis, yet it is unclear how it functions. F. tularensis also contains an MglA-like protein called SspA. Here, we show that MglA and SspA cooperate with one another to control virulence gene expression in F. tularensis. Using a directed proteomic approach, we show that both MglA and SspA associate with RNA polymerase (RNAP) in F. tularensis, and that SspA is required for MglA to associate with RNAP. Furthermore, bacterial two-hybrid and biochemical assays indicate that MglA and SspA interact with one another directly. Finally, through genome-wide expression analyses, we demonstrate that MglA and SspA regulate the same set of genes. Our results suggest that a complex involving both MglA and SspA associates with RNAP to positively control virulence gene expression in F. tularensis. The F. tularensis genome is unusual in that it contains two genes encoding different α subunits of RNAP, and we show here that these two α subunits are incorporated into RNAP. Thus, as well as identifying SspA as a second critical regulator of virulence gene expression in F. tularensis, our findings provide a framework for understanding the mechanistic basis for virulence gene control in a bacterium whose transcription apparatus is unique

Public vulnerability to the police: a quantitative inquiry

The recent protests regarding the state of policing in the United States clearly demonstrate that how the police do their job creates a salient potential for harm to the public. This study applies a multidimensional paradigm of risk perception to quantify evaluations of police-caused harm. Using data from a national (U.S.) convenience sample (n = 1,890) that oversampled individuals who self-identified as black or Muslim, we tested whether these evaluations vary systematically (using confidence intervals), whether they covary with police legitimacy (using structural equation modeling), and the extent to which that covariance differs by demographic status (using multiple groups structural equation modeling). Our results suggest that black and Muslim individuals evaluate police-caused harm differently than do majority group members (white and Christian) on most, but not all, of the measured dimensions. We also find that those evaluations are predictive of trust and provide evidence of some level of consistency across communities

Twin RNA Polymerase–Associated Proteins Control Virulence Gene Expression in Francisella tularensis

The MglA protein is the only known regulator of virulence gene expression in Francisella tularensis, yet it is unclear how it functions. F. tularensis also contains an MglA-like protein called SspA. Here, we show that MglA and SspA cooperate with one another to control virulence gene expression in F. tularensis. Using a directed proteomic approach, we show that both MglA and SspA associate with RNA polymerase (RNAP) in F. tularensis, and that SspA is required for MglA to associate with RNAP. Furthermore, bacterial two-hybrid and biochemical assays indicate that MglA and SspA interact with one another directly. Finally, through genome-wide expression analyses, we demonstrate that MglA and SspA regulate the same set of genes. Our results suggest that a complex involving both MglA and SspA associates with RNAP to positively control virulence gene expression in F. tularensis. The F. tularensis genome is unusual in that it contains two genes encoding different α subunits of RNAP, and we show here that these two α subunits are incorporated into RNAP. Thus, as well as identifying SspA as a second critical regulator of virulence gene expression in F. tularensis, our findings provide a framework for understanding the mechanistic basis for virulence gene control in a bacterium whose transcription apparatus is unique