The Hyrum and Beirdneau Formations of North-Central Utah and Southeastern Idaho

The Hyrum and Beirdneau Formations of North-central Utah and vi Southeastern Idaho represent rocks of late Middle Devonian (Givetian) to upper Upper Devonian (Famennian) age. They are disconformably underlain by the Early Devonian Water Canyon Formation in most cases and disconformably overlain by the Devonian-Mississippian Leatham Formation or the Mississippian Lodgepole Formation. The Hyrum Formation is divided into five members based on lithology and color changes. The five members are: (1) Samaria, (2) Lower Dolomite, (3) Lower Carbonate-detritus, (4) Upper Dolomite, and (5) Upper Carbonate-detritus Members. The Samaria Member is the only fossiliferous unit within the Hyrum Formation. The Beirdneau Formation is divided into three members based on lithology and color cha~ges. These three members Sre: (1) Lower Carbonate, (2) Sandstone, and (3) Upper Carbonate Members. This Formation represents deposition in a shallow water and a restricted sea environment

Radiometric performance of AVIRIS: Assessment for an arid region geologic target

Data from several AVIRIS flight lines were examined to assess instrument stability and response. Both scene and in-flight calibration data were analyzed statistically. The data clearly indicates that, although the instrument output was noisy and unstable at the time of the data acquisition, valuable spectral signatures can still be extracted and analyzed. Some first order calibration corrections can be performed by forcing internal consistency within the data. AVIRIS data are delivered in band-interleaved-by-line format, but high efficiency routines were developed which access the data as either image or spectral planes and enable effective statistical and visual examination of both AVIRIS scenes and ancillary files. Two methods were used to extract spectral information from segment 4 of the Kelso Dunes flight. Both successfully identified at least three distinct spectral signatures, but neither has positively identified a specific material

Transcriptional profiling of degraded RNA in cryopreserved and fixed tissue samples obtained at autopsy

BACKGROUND: Traditional multiplexed gene expression methods require well preserved, intact RNA. Such specimens are difficult to acquire in clinical practice where formalin fixation is the standard procedure for processing tissue. Even when special handling methods are used to obtain frozen tissue, there may be RNA degradation; for example autopsy samples where degradation occurs both pre-mortem and during the interval between death and cryopreservation. Although specimens with partially degraded RNA can be analyzed by qRT-PCR, these analyses can only be done individually or at low levels of multiplexing and are laborious and expensive to run for large numbers of RNA targets. METHODS: We evaluated the ability of the cDNA-mediated Annealing, Selection, extension, and Ligation (DASL) assay to provide highly multiplexed analyses of cryopreserved and formalin fixed, paraffin embedded (FFPE) tissues obtained at autopsy. Each assay provides data on 1536 targets, and can be performed on specimens with RNA fragments as small as 60 bp. RESULTS: The DASL performed accurately and consistently with cryopreserved RNA obtained at autopsy as well as with RNA extracted from formalin-fixed paraffin embedded tissue that had a cryopreserved mirror image specimen with high quality RNA. In FFPE tissue where the cryopreserved mirror image specimen was of low quality the assay performed reproducibly on some but not all specimens. CONCLUSION: The DASL assay provides reproducible results from cryopreserved specimens and many FFPE specimens obtained at autopsy. Gene expression analyses of these specimens may be especially valuable for the study of non-cancer endpoints, where surgical specimens are rarely available

Gene expression profiling revealed novel mechanism of action of Taxotere and Furtulon in prostate cancer cells

BACKGROUND: Both Taxotere and Capecitabine have shown anti-cancer activity against various cancers including prostate cancer. In combination, Taxotere plus Capecitabine has demonstrated higher anti-cancer activity in advanced breast cancers. However, the molecular mechanisms of action of Taxotere and Capecitabine have not been fully elucidated in prostate cancer. METHODS: The total RNA from PC3 and LNCaP prostate cells untreated and treated with 2 nM Taxotere, 110 μM Furtulon (active metabolite of Capecitabine), or 1 nM Taxotere plus 50 μM Furtulon for 6, 36, and 72 hours, was subjected to Affymetrix Human Genome U133A Array analysis. Real-time PCR and Western Blot analysis were conducted to confirm microarray data. RESULTS: Taxotere and Furtulon down-regulated some genes critical for cell proliferation, cell cycle progression, transcription factor, cell signaling, and oncogenesis, and up-regulated some genes related to the induction of apoptosis, cell cycle arrest, and differentiation in both cell lines. Taxotere and Furtulon also up-regulated some genes responsible for chemotherapeutic resistance, suggesting the induction of cancer cell resistance to these agents. CONCLUSIONS: Taxotere and Furtulon caused the alternation of a large number of genes, many of which may contribute to the molecular mechanisms by which Taxotere and Furtulon inhibit the growth of prostate cancer cells. This information could be utilized for further mechanistic research and for devising optimized therapeutic strategies against prostate cancer

Laser Scanning Cytometry for selection of green fluorescent protein transgenic mice using small number of blood cells

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