Physiology and natural distribution of the bacterium caryophanon latum in fresh waters of Missouri

Students supported: 2 B.S. Students SupportedThe objective of this research program was to study the physiology of Caryophanon latum and to develop a selective medium for growing the organism to the exclusion of most, if not all, other organisms found in stream water. After development of this selective medium, the procedure was to be evaluated on sample stream waters with and without inoculation with the organism. The primary goal of this project is the development of a technique for the detection and possible enumeration of organisms of the genus Carophanon in natural waters. Demonstration of the presence of these organisms would prove fecal pollution by ruminants and enumeration methods would make it possible to locate the point of pollution.Project # A-048-MO Agreement # 14-31-0001-352

Hubble Space Telescope observations of Mars

Hubble Space Telescope (HST) afforded the possibility of resolving features as small as 100 km on the Martian surface even when it is at the far point of its orbit. Therefore it is ideally suited for monitoring seasonal changes on the red planet. The objectives research include: the study of Martian dust storms; use of images obtained through different filters to study the spectral reflectance of regions on the Martian surface; use of ultraviolet images and spectra to measure the amount of ozone in the planet's atmosphere as a function of location of the planet; use of images to study changes in the albedo of the Mars surface; and use of Planetary Camera images to study Martian clouds and to measure the opacity of the atmosphere

Rapid, Low-Cost Conversion from Rice to Improved Pastures.

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Evaluation of the optical switching characteristics of erbium-doped fibres for the development of a fibre Bragg grating sensor interrogator

A polling topology that employs optical switching based on the properties of erbium-doped fibres (EDFs) is used to interrogate an array of FBGs. The properties of the EDF are investigated in its pumped and un-pumped states and the EDFs’ switching properties are evaluated by comparing them with a high performance electronically controlled MEM optical switch. Potential advantages of the proposed technique are discussed. © (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only

A Role for the SmpB-SsrA System in Yersinia pseudotuberculosis Pathogenesis

Yersinia utilizes a sophisticated type III secretion system to enhance its chances of survival and to overcome the host immune system. SmpB (small protein B) and SsrA (small stable RNA A) are components of a unique bacterial translational control system that help maintain the bacterial translational machinery in a fully operational state. We have found that loss of the SmpB-SsrA function causes acute defects in the ability of Yersinia pseudotuberculosis to survive in hostile environments. Most significantly, we show that mutations in smpB-ssrA genes render the bacterium avirulent and unable to cause mortality in mice. Consistent with these observations, we show that the mutant strain is unable to proliferate in macrophages and exhibits delayed Yop-mediated host cell cytotoxicity. Correspondingly, we demonstrate that the smpB-ssrA mutant suffers severe deficiencies in expression and secretion of Yersinia virulence effector proteins, and that this defect is at the level of transcription. Of further interest is the finding that the SmpB-SsrA system might play a similar role in the related type III secretion system that governs flagella assembly and bacterial motility. These findings highlight the significance of the SmpB-SsrA system in bacterial pathogenesis, survival under adverse environmental conditions, and motility