An assessment of haematological and serum biochemical indices in Salmo trutta caspius

Haematological and biochemical serum analyses were carried out in 131 fish specimens in three age and size groups including smolt, juvenile and breeder. Of these groups, smolt fish were 40 specimens in the average total length range 195.45±12.426mm and had an average weight of 84±15.5g, juvenile fish were 25 individuals and their average total length and weight were 267±20.877mm and 217.20±22.013g, respectively. The 41 breeder fish had an average total length of 578.24±68.487mm and an average weight of 1994.2±775.379g. Another 25 breeder fish were also sampled in the spawning season for blood biochemical analyses had an average total length range of 617±59.9mm and weighed on average 2227.5±647.5g each. Red blood cell counts were 866600 per mm super(3) and 1259400 per mm ^(3) in smolt and breeder fish respectively. The average hematocrit was 48.39% in smolt and 44.29% in breeder fish. The average hemoglobin was 8.85g/dl in smolt and 10.91g/dl in breeder fish. White blood cell count was 8781.58 per mm ^(3) in smolt and 5217.65 per mm ^(3) in breeder fish. Other measurements were as follows: Lymphocyte 90.57% in smolt and 73.22% in breeders, Neutrophil 5.12% in smolt and 16.92% in breeders, Monocyte 1.27% in smolt and 4.24% in breeders, and clotting time was 282.34 seconds in smolt and 291.47 seconds in breeder fish. We also measured MCV, MCH and MCHC in smolt and breeder fish along with biochemical parameters. The glucose level was 2.97mmol/1 in juvenile and 1.99mmol/1 in breeder fish. The cholesterol level was 4.26mmol/l in juvenile and 7.06mmol/1 in breeders. The triglyceride amount was 2.35mmol/l in juvenile and 2.47mmol/l in breeder specimens and the calcium level was 2.44 in juvenile and 2.61 mmol/1 in breeder fish

Comparison of produced fish protein hydrolysate from viscera and head of silver carp (Hypophthalmichthys molitrix) using alcalase enzyme and internal tissue enzymes

In the present study, hydrolysed protein of viscera and head of Silver carp (Hypophthalmichthys molitrix) was compared using Alcalase enzyme and internal tissue enzymes at 2 and 4 hours. The result indicated that product by Alcalase (Treatment 1) had significantly higher protein and rate of hydrolysates than that produced by internal tissue enzymes (Treatment 2). So, the highest mean (±SD) protein (68.10±1.33) was related to treatment 1-head (with Alcalase enzyme) after 4 hours and the highest rate of hydrolysates (29.36±1.35) was related to treatment 1-head (with Alcalase enzyme) after 4 hours. The result indicated that rate of hydrolysates raised as time of hydrolysates increased. However the intensity and rate of hydrolysates is reduced. The highest rate of hydrolysates occurred at 120 minutes in the first. This mode was similar for two treatments. The result can be considered as the Alcalase was preferred to internal enzyme

Study on some hematological factors of Salmo trutta caspius

Regarding to measure some hematological factors of Caspian Salmon (Salmo Trutta caspius), 55 immature and 21 mature individuals were studied. The main results were: Immature Mature - Hemoglobin value (gr/dl) 7.20 9.87 - Hematocrit 34.1 39.2 - Mean No. of red blood cells (per mm3) 934300 1306200 - Mean No. of white blood (per mm3) 8380 6219 - Mean No. of platelets (per mm3) 21180 13159 As the results showed the number of white blood cells and platelets in immature individuals were more than mature specimen. The obtained results of blood indices were as follow: Immature Mature - Mean MCV (FL) 385.4 302.9 - Mean MCH (Pg) 82.0 75.5 - Mean MCHC (%) 21.6 25.6 - Lymphocyte (%) 96.1 69.9 - Neutrophil (%) 3.4 29.4 - Monocyte (%) 0.52 1.14 Notice: Eosinophil and basophil cells weren't observed