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An assessment of haematological and serum biochemical indices in Salmo trutta caspius

Abstract

Haematological and biochemical serum analyses were carried out in 131 fish specimens in three age and size groups including smolt, juvenile and breeder. Of these groups, smolt fish were 40 specimens in the average total length range 195.45±12.426mm and had an average weight of 84±15.5g, juvenile fish were 25 individuals and their average total length and weight were 267±20.877mm and 217.20±22.013g, respectively. The 41 breeder fish had an average total length of 578.24±68.487mm and an average weight of 1994.2±775.379g. Another 25 breeder fish were also sampled in the spawning season for blood biochemical analyses had an average total length range of 617±59.9mm and weighed on average 2227.5±647.5g each. Red blood cell counts were 866600 per mm super(3) and 1259400 per mm ^(3) in smolt and breeder fish respectively. The average hematocrit was 48.39% in smolt and 44.29% in breeder fish. The average hemoglobin was 8.85g/dl in smolt and 10.91g/dl in breeder fish. White blood cell count was 8781.58 per mm ^(3) in smolt and 5217.65 per mm ^(3) in breeder fish. Other measurements were as follows: Lymphocyte 90.57% in smolt and 73.22% in breeders, Neutrophil 5.12% in smolt and 16.92% in breeders, Monocyte 1.27% in smolt and 4.24% in breeders, and clotting time was 282.34 seconds in smolt and 291.47 seconds in breeder fish. We also measured MCV, MCH and MCHC in smolt and breeder fish along with biochemical parameters. The glucose level was 2.97mmol/1 in juvenile and 1.99mmol/1 in breeder fish. The cholesterol level was 4.26mmol/l in juvenile and 7.06mmol/1 in breeders. The triglyceride amount was 2.35mmol/l in juvenile and 2.47mmol/l in breeder specimens and the calcium level was 2.44 in juvenile and 2.61 mmol/1 in breeder fish

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