Electrochemically Pretreated Carbon Microfiber Electrodes as Sensitive HPLC-EC Detectors

The paper focuses on the analysis and detection of electroactive compounds using high-performance liquid chromatography (HPLC) combined with electrochemical detection (EC). The fabrication and utilization of electrochemically treated carbon fiber microelectrodes (CFMs) as highly sensitive amperometric detectors in HPLC are described. The applied pretreatment procedure is beneficial for analytical characteristics of the sensor as demonstrated by analysis of the model set of phenolic acids. The combination of CFM with separation power of HPLC technique allows for improved detection limits due to unique electrochemical properties of carbon fibers. The CFM proved to be a promising tool for amperometric detection in liquid chromatography

Neurogenic pulmonary oedema as a rare complication of epileptic seizures

Introduction: Neurogenic pulmonary oedema (NPE) is a very rare complication of epileptic seizures, which could potentially increase mortality.Material and methods: The case of a 66-year-old male patient with NPE caused by repeated epileptic seizures is reported. Rapid resolution of pulmonary oedema is well documented by X-ray and computed tomography images.Conclusions: Neurogenic pulmonary oedema could potentially increase mortality, and thus, it is important to perform a chest X-ray in all patients presenting with seizures and dyspnoea

Hematene: A sustainable 2D conductive platform for visible-light-driven photocatalytic ammonia decomposition

The emerging class of 2D non-van der Waals (n-vdW) materials, including 2D iron oxides, possesses unique properties and high applicability, making them attractive for various technological applications. However, the synthesis of these materials through a scalable and eco-friendly method remains a challenge, as most known chemical exfoliation processes require toxic organic solvents. In this study, we report a green synthesis of 2D hematene (α-Fe2O3) using an ultrasound-supported exfoliation method of earth-abundant iron oxide ore in a pure aqueous solution. The resulting hematene sheets, only a few nanometers thick, exhibit superior electrochemical performance in terms of charge transfer processes, making them ideal for photocatalytic applications. By doping a conductive hematene substrate with ruthenium, we demonstrate a synergistic effect for generating electrons and holes under visible light irradiation. Using this approach, we successfully decomposed ammonia into hydrogen and nitrogen, highlighting the potential of this novel class of environmentally-friendly photocatalysts for clean energy production. Overall, our water-assisted scalable synthesis of hematene offers a promising strategy for producing efficient and sustainable photocatalysts.Web of Science34art. no. 10188

Click and detect: Versatile ampicillin aptasensor enabled by click chemistry on a graphene-alkyne derivative

Tackling the current problem of antimicrobial resistance (AMR) requires fast, inexpensive, and effective methods for controlling and detecting antibiotics in diverse samples at the point of interest. Cost-effective, disposable, point-of care electrochemical biosensors are a particularly attractive option. However, there is a need for conductive and versatile carbon-based materials and inks that enable effective bioconjugation under mild conditions for the develop ment of robust, sensitive, and selective devices. This work describes a simple and fast methodology to construct an aptasensor based on a novel graphene derivative equipped with alkyne groups prepared via fluorographene chem istry. Using click chemistry, an aptamer is immobilized and used as a suc cessful platform for the selective determination of ampicillin in real samples in the presence of interfering molecules. The electrochemical aptasensor displayed a detection limit of 1.36 nM, high selectivity among other antibi otics, the storage stability of 4 weeks, and is effective in real samples. Addi tionally, structural and docking simulations of the aptamer shed light on the ampicillin binding mechanism. The versatility of this platform opens up wide possibilities for constructing a new class of aptasensor based on disposable screen-printed carbon electrodes usable in point-of-care devices.Web of Scienc

PDBe-KB: collaboratively defining the biological context of structural data

The Protein Data Bank in Europe - Knowledge Base (PDBe-KB, https://pdbe-kb.org) is an open collaboration between world-leading specialist data resources contributing functional and biophysical annotations derived from or relevant to the Protein Data Bank (PDB). The goal of PDBe-KB is to place macromolecular structure data in their biological context by developing standardised data exchange formats and integrating functional annotations from the contributing partner resources into a knowledge graph that can provide valuable biological insights. Since we described PDBe-KB in 2019, there have been significant improvements in the variety of available annotation data sets and user functionality. Here, we provide an overview of the consortium, highlighting the addition of annotations such as predicted covalent binders, phosphorylation sites, effects of mutations on the protein structure and energetic local frustration. In addition, we describe a library of reusable web-based visualisation components and introduce new features such as a bulk download data service and a novel superposition service that generates clusters of superposed protein chains weekly for the whole PDB archive

PDBe-KB: collaboratively defining the biological context of structural data

The Protein Data Bank in Europe – Knowledge Base (PDBe-KB, https://pdbe-kb.org) is an open collaboration between world-leading specialist data resources contributing functional and biophysical annotations derived from or relevant to the Protein Data Bank (PDB). The goal of PDBe-KB is to place macromolecular structure data in their biological context by developing standardised data exchange formats and integrating functional annotations from the contributing partner resources into a knowledge graph that can provide valuable biological insights. Since we described PDBe-KB in 2019, there have been significant improvements in the variety of available annotation data sets and user functionality. Here, we provide an overview of the consortium, highlighting the addition of annotations such as predicted covalent binders, phosphorylation sites, effects of mutations on the protein structure and energetic local frustration. In addition, we describe a library of reusable web-based visualisation components and introduce new features such as a bulk download data service and a novel superposition service that generates clusters of superposed protein chains weekly for the whole PDB archive

Manipulation robot controlled by gestures

Cílem této práce je navrhnout mechanický manipulátor ovládaný gesty svhodnou kinematickou strukturou a řešením pro ovládání. Pro rozpoznání gest jevyužito strojového vidění a neuronových sítí. V práci je shrnuta rešerše hardwarovýchvývojových desek, seznámení se s ovládáním prostorových mechanismů a kalibrace.The aim of this thesis is to design a mechanical manipulator controlled bygestures with suitable kinematic structure. For gesture recognition is used computervision and neural networks. This work features research of hardware componentssuch as development board, familiarization with control of spatial mechanisms andcalibration

Essai sur le sens de l'hiatus dans la poésie de Mácha et de Holan

Jakubec David France. Essai sur le sens de l'hiatus dans la poésie de Mácha et de Holan. In: Revue des études slaves, tome 60, fascicule 4, 1988. pp. 829-837

Using UML as a tool for PLC programing

Cílem této práce je navrhnout způsob generování kódu ze softwarového jazyka UML nebo SysML pro řídicí systémy PLC. V práci je nejdříve rešerše na toto téma. Popisuje co UML je a jaké diagramy je dobré na co používat. Dále jsou sestaveny modely pro některé školní úlohy pro předmět řízení programovatelnými automaty. Tyto modely jsou poté podle návrhu napsány v jazyce Strukturovaného Textu podle toho, jak by mohly být generované.The aim of this work is to design a method of generating code using software languages UML and/or SysML for PLC automation. This research primarily focuses on the following topics. Describing what UML is and which diagrams work best with it. Furthermore, designed models for a few school tasks on the subject of Programmable Controller Applications. Afterwards, these models are programmed in Structured Text based on how they could be generated

jakubec_plosone_2016.tar.gz

Data set of interaction energies of amino acid--DNA base and amino acid--deoxynucleotide dimers in various dielectric environments.<br