Design and Analysis of High Speed Multiply and Accumulation Unit for Digital Signal Processing Applications

Unit for Digital Signal Processing Applications   Kausar Jahan1, Pala Kalyani2, V Satya Sai3, GRK Prasad4, Syed Inthiyaz5, Sk Hasane Ahammad6 1Department of ECE, Dadi Institute of Engineering and Technology Anakapalle, Andhra Pradesh, India 2Department of ECE, Vardhaman College of Engineering Kacharam, Shamshabad, India 3Department of ECE, Koneru Lakshmaiah Education Foundation Guntur, India-522502 4Department of ECE, Koneru Lakshmaiah Education Foundation Guntur, India-522502 5Department of ECE, Koneru Lakshmaiah Education Foundation Guntur, India-522502 6Department of ECE, Koneru Lakshmaiah Education Foundation Guntur, India-522502   Abstract—The fundamental component used in many of the Digital signal Processing (DSP) applications are Multiply and Accumulation Unit (MAC). In the literature, a multiplier consists of greater number of full adders and half adder in partial product reduction stage, which increases the hardware complexity and critical path delay to MAC unit. To overcome this problem, two novel multipliers are proposed in this article. The proposed multipliers are designed and implemented in hardware, which reduces the circuit complexity and improves the overall performance of the MAC unit with less delay. The proposed multipliers are compared with the 4-bit existing designs and observed that the number of slices Look Up Tables (LUTs) are minimized from 113 to 43, Slices are reduced from 46 to 14, Full Adders (FAs) are lessened from 28 to 23, bonded Input Output Blocks (IOBs) and Half Adders (HAs) were not altered. The time delay is reduced from 14.251ns to 7.876ns. The proposed multipliers are compared in the literature with the 8-bit multiplier, then the number of Slice LUTs are reduced from 510 to 231, Slices are reduced from 218 to 113, FAs are reduced from 120 to 110, HAs are reduced from 56 to 39, time delay is reduced from 26.228ns to12.748ns, but bonded IOBs count remains same. The synthesis and simulations results are verified by using Xilinx ISE 14.7 version tool

Purification and medium optimization of α-amylase from Bacillus subtilis 168

α-Amylase was first time isolated and purified from Bacillus subtilis 168 (1A1). Purified α-amylase fraction showed a single protein band with a molecular weight of 55 kD. Chemical characterization of the purified α-amylase revealed optimum amylolytic activity at 37°C and pH 7.0 using starch as substrate. It was stable at pH 5.0 to 9.0 and at temperatures 25–70°C. Culture conditions were optimized by using statistics-based experimental designs to enhanced α-amylase (EC.3.2.1.1) production. A two level fractional factorial Plackett-Burman design was used for the preliminary screening significant media components and conditions. Response surface methodology (RSM) involving a 24 full-factorial central composite design (CCD) and a second-order polynomial equation was then employed to identify the relationship between the α-amylase yield and the four significant variables. Optimal levels of the significant variables for the maximum α-amylase yield were starch 2.55 g/l, yeast extract 8.4 g/l, sodium chloride 8.1% and 48 h of incubation. Mean value of α-amylase yield was 639.7 IU/ml, which was in excellent agreement with the predicted value (633.5 IU/ml).Key words: Bacillus, α-amylase, optimization, Plackett-Burman design, response surface methodology

A brief review on molecular, genetic and imaging techniques for HCV fibrosis evaluation

<p>Abstract</p> <p>Background</p> <p>Chronic HCV is one of the major causes of morbidity and mortality in the present day world. The assessment of disease progression not only provides useful information for diagnosis and therapeutic supervision judgment but also for monitoring disease. Different invasive and non invasive methods are applied to diagnose the disease from initial to end stage (mild fibrosis to cirrhosis). Although, liver biopsy is still considered as gold standard to identify liver histological stages, an assessment of the disease development based on non-invasive clinical findings is also emerging and this may replace the need of biopsy in near future. This review gives brief insight on non-invasive methods currently available for predicting liver fibrosis in HCV with their current pros and cons to make easier for a clinician to choose better marker to assess liver fibrosis in HCV infected patients.</p> <p>Methods</p> <p>More than 200 studies regarding invasive and noninvasive markers available for HCV liver disease diagnosis were thoroughly reviewed. We examined year wise results of these markers based on their sensitivity, specificity, PPV, NPV and AUROCs.</p> <p>Results</p> <p>We found that in all non-invasive serum markers for HCV, FibroTest, Forn's Index, Fibrometer and HepaScore have high five-year predictive value but with low AUROCs (0.60~0.85) and are not comparable to liver biopsy (AUROC = 0.97). Even though from its beginning, Fibroscan is proved to be best with high AUROCs (> 0.90) in all studies, no single noninvasive marker is able to differentiate all fibrosis stages from end stage cirrhosis. Meanwhile, specific genetic markers may not only discriminate fibrotic and cirrhotic liver but also differentiate individual fibrosis stages.</p> <p>Conclusions</p> <p>There is a need of marker which accurately determines the stage based on simplest routine laboratory test. Genetic marker in combination of imaging technique may be the better non invasive diagnostic method in future.</p

A comparison of four fibrosis indexes in chronic HCV: Development of new fibrosis-cirrhosis index (FCI)

<p>Abstract</p> <p>Background</p> <p>Hepatitis C can lead to liver fibrosis and cirrhosis. We compared readily available non-invasive fibrosis indexes for the fibrosis progression discrimination to find a better combination of existing non-invasive markers.</p> <p>Methods</p> <p>We studied 157 HCV infected patients who underwent liver biopsy. In order to differentiate HCV fibrosis progression, readily available AAR, APRI, FI and FIB-4 serum indexes were tested in the patients. We derived a new fibrosis-cirrhosis index (FCI) comprised of ALP, bilirubin, serum albumin and platelet count. FCI = [(ALP × Bilirubin) / (Albumin × Platelet count)].</p> <p>Results</p> <p>Already established serum indexes AAR, APRI, FI and FIB-4 were able to stage liver fibrosis with correlation coefficient indexes 0.130, 0.444, 0.578 and 0.494, respectively. Our new fibrosis cirrhosis index FCI significantly correlated with the histological fibrosis stages F0-F1, F2-F3 and F4 (r = 0.818, p < 0.05) with AUROCs 0.932 and 0.996, respectively. The sensitivity and PPV of FCI at a cutoff value < 0.130 for predicting fibrosis stage F0-F1 was 81% and 82%, respectively with AUROC 0.932. Corresponding value of FCI at a cutoff value ≥1.25 for the prediction of cirrhosis was 86% and 100%.</p> <p>Conclusions</p> <p>The fibrosis-cirrhosis index (FCI) accurately predicted fibrosis stages in HCV infected patients and seems more efficient than frequently used serum indexes.</p

Effects of methanol in blood pressure and heart rate in the rat

Introduction: Methanol ingestion is an uncommon form of poisoning that can cause severe metabolic disturbances, blindness, permanent neurologic dysfunction and death. While methanol itself may be harmless, it converts, in vivo, to highly toxic formic acid. Methanol intoxication clinically manifests as ocular toxicity. The present study investigated the cardiovascular effects of methanol. Materials and Methods: On the day of the experiment, Wistar rats were anesthetized with urethane. The femoral artery on one side was exposed, and a polyethylene catheter was inserted into the artery for recording arterial blood pressure (ABP). The catheter was attached to a pressure transducer (Statham - P23D). Systolic blood pressure (BP), mean ABP, and heart rate were recorded on a power-lab data acquisition system with a computerized analysis program. Rats were administered with different dilutions (9.5%, 19.0%, 28.5%, 38.0%, 47.5%, 57.0%, 66.5%, 76%) of methanol (95% v/v, i.v.). Results: Of all dilutions of methanol, 66.5% dilution showed maximum decrease of diastolic BP from 124.64 ± 5.39 to 62.30 ± 11.90 mmHg; 76.0% dilution showed maximum decrease of systolic BP from 165.70 ± 5.57 to 112.11 ± 12.0 mmHg, and mean ABP from 160.61 ± 12.45 to 86.14 ± 4.11 mmHg. The heart rate increased (from 250 beats/s to near about 275 beats/s) following administration of methanol dilution from 19.0% till 76.0%. Conclusion: The present study is consistent with previous studies suggesting that methanol ingestion leads to severe hypotension as observed from decrease in diastolic BP, systolic BP, and mean ABP. However, severe increase of heart rate suggests activation of a compensatory mechanism to offset hypotension that eventually leads to death in methanol poisoning. Hence, this study emphasizes the need to monitor all the hemodynamic parameters in accidental methanol poisoning

Inter-observer agreement for detection and grading of hepatic steatosis- An ultrasound based study

Objectives: To determine the inter observer agreement of ultrasound diagnosis of the severity and grading of steatosis in patients with HCV.Methods: Patients with HCV were evaluated, from March 2008-August 2010 at Radiology department, Dow Medical College and Civil Hospital Karachi, with ultrasound for detection and grading of steatosis and fibrosis using a standardized set of criteria. The same sets of images were reviewed by the same radiologists 5 years later (2015) for determining the agreement in the grading. Kappa (k) statistics were utilized. Present results were compared with that of the pilot study conducted in 2007 on 100 images.Results: 452 set of images were reviewed by three radiologists, designated A, B and C. The inter observer agreement was satisfactory to good with k=0.8 for no steatosis, 0.4 for mild steatosis, 0.7 for moderate steatosis and 0.9 for severe steatosis. The inter-observer agreement in the pilot study in 2007 had shown k – values of 0.81 for no steatosis, 0.5 for mild steatosis, 0.8 for moderate steatosis and 0.9 for severe steatosis.Conclusions: The studied criteria for ultrasound diagnosis and grading of hepatic steatosis had persistently good inter-observer agreement for absence, and moderate to severe grades of steatosis. The agreement was low for mild steatosis

Invitro studies on the potential Trichoderma harzianum for antaganostic properties against Ganoderma boninnese.

Ganoderma boninense is a bracket fungus that is pathogenic on oil palms causing basal stem rot, while some members of the genus Trichoderma are good biological control agents against numerous plant fungal diseases. We isolated and tested T. harzianum forin-vivonursery to suppress diseases caused by G. boninense. A total of 48local isolates of T. harzianum were selected for screening of their antagonistic properties againstG. boninense(strain PER 71) by dual culture techniques. The results showed that all of the isolates of T. harzianum inhibited the growth of PER 71 with percentage of inhibition radial growth (PIRG) values ranging from 47.86 to 72.06%. The best eight samples from the dual cultures were then tested for their production of volatile antifungal compounds against PER 71, which gave PIRG values between 24.53 and 58.70% over 6 days. The values ranged from 18.35 to 40.16% over 6 days for the antifungal activities of their non-volatile compounds. Isolate FA 30 was demonstrated to be the best isolate not only to the dual culture inhibition tests but also the best for the production of inhibitory properties from both volatile and non-volatile antifungal compounds

Effect of addition of mosambi (Citrus limetta) peel powder on textural and sensory properties of papaya jam

This study evaluates the influence of addition of mosambi peel powder on jam prepared from papaya. Mosambi peel powder was first analyzed for its proximate composition and other properties. Mosambi peel had high amount of crude fiber (17.6%), besides water and oil-holding capacity (2.26 and 6.82 ml/g, respectively). Mosambi peel was treated with 5% of salt and/or sodium bicarbonate overnight to remove bitterness. Different levels of treated and untreated mosambi peel powder (2.5, 5, 7.5, 10, and 12.5%) were added to papaya jam and were evaluated for texture and sensory properties. The firmness and chewiness values of the jam added with mosambi peel powder increased significantly as compared to control, whereas adhesiveness and cohesiveness values decreased with increasing levels of mosambi peel powder. Sensory evaluation showed that jam prepared by addition of peel powder was acceptable up to 5% level of incorporation. However, jam made by the addition of untreated peel powder was not acceptable due to bitterness resulted from mosambi peel powder

Effect of addition of mosambi (Citrus limetta) peel powder on textural and sensory properties of papaya jam ABOUT THE AUTHORS

Abstract: This study evaluates the influence of addition of mosambi peel powder on jam prepared from papaya. Mosambi peel powder was first analyzed for its proximate composition and other properties. Mosambi peel had high amount of crude fiber (17.6%), besides water and oil-holding capacity (2.26 and 6.82 mL/g, respectively). Mosambi peel was treated with 5% of salt and/or sodium bicarbonate overnight to remove bitterness. Different levels of treated and untreated mosambi peel powder (2.5, 5, 7.5, 10, and 12.5%) were added to papaya jam and were evaluated for texture and sensory properties. The firmness and chewiness values of the jam added with mosambi peel powder increased significantly as compared to control, whereas adhesiveness and cohesiveness values decreased with increasing levels of mosambi peel powder. Sensory evaluation showed that jam prepared by addition of peel powder was acceptable up to 5% level of incorporation. However, jam made by the addition of untreated peel powder was not acceptable due to bitterness resulted from mosambi peel powder

Design of Model-Based and Model-Free Robust Control Strategies for Lower Limb Rehabilitation Exoskeletons

Rehabilitation in the form of locomotion assistance and gait training through robotic exoskeletons requires both precision and accuracy to achieve effective results. The essential challenge is to ensure robust tracking of the reference signal, i.e., of the gait or locomotion. This paper presents the design of model-based (MB) and model-free (MF) robust control strategies to achieve desired performance and robustness in terms of transient behavior and steady-state/tracking error, implementable to the locomotion assistance and gait training by exoskeletons. The dynamic responses of the exoskeleton system were investigated with both the control strategies. The study was carried out with a variety of reference signals and performance was evaluated to identify the best suited approach for rehabilitation exoskeletons. In case of the model-based control, a mathematical model of the system was developed using a bond graph modeling technique and a lead compensated H-infinity reference gain controller was designed to ensure robust tracking performance. In the model-free control strategy, however, the system function is approximated using radial basis function neural networks (RBFNNs) and an adaptive proportional-derivative RBFNN controller was designed to achieve the desired results with minimum tracking error. Both strategies make the system robust and stable. However, the MF control strategy is faster for all reference inputs as compared to the MB control strategy i.e., faster to approach the peak value and settle, and rapidly approaches the zero steady-state/tracking error. The rise time in the case of a sinusoidal input for model-free control is 0.4 s faster than the rise time in model-based control. Similarly, the settling time is 3.9 s faster in the case of model-free control, which is a prominent difference and can provide better rehabilitation results