Effectiveness Study On Temporary Pavement Marking Removals Methods

This study was conducted to identify effective temporary marking removal methods and procedures on concrete and asphalt pavements. Pavement markings provide guidance to road travelers and can lead to accidents when not properly removed. Current state guidelines on removal do not provide clear and objective methods of measurement. After testing the most common removal methods, this research concluded that removing markings by chemical was not only cost and results-oriented effective compared to other methods, but it was also safe to the environment and road users. Finally, a baseline of measurements was developed by the research team for this project, along with the feasibility of using digital image processing to objectively determine whether or not a removal method could be deemed effective

Effectiveness Study On Temporary Pavement Marking Removals Methods

This study was conducted to identify effective temporary marking removal methods and procedures on concrete and asphalt pavements. Pavement markings provide guidance to road travelers and can lead to accidents when not properly removed. Current state guidelines on removal do not provide clear and objective methods of measurement. After testing the most common removal methods, this research concluded that removing markings by chemical was not only cost and results-oriented effective compared to other methods, but it was also safe to the environment and road users. Finally, a baseline of measurements was developed by the research team for this project, along with the feasibility of using digital image processing to objectively determine whether or not a removal method could be deemed effective

PDB-Ligand: a ligand database based on PDB for the automated and customized classification of ligand-binding structures

PDB-Ligand (http://www.idrtech.com/PDB-Ligand/) is a three-dimensional structure database of small molecular ligands that are bound to larger biomolecules deposited in the Protein Data Bank (PDB). It is also a database tool that allows one to browse, classify, superimpose and visualize these structures. As of May 2004, there are about 4870 types of small molecular ligands, experimentally determined as a complex with protein or DNA in the PDB. The proteins that a given ligand binds are often homologous and present the same binding structure to the ligand. However, there are also many instances wherein a given ligand binds to two or more unrelated proteins, or to the same or homologous protein in different binding environments. PDB-Ligand serves as an interactive structural analysis and clustering tool for all the ligand-binding structures in the PDB. PDB-Ligand also provides an easier way to obtain a number of different structure alignments of many related ligand-binding structures based on a simple and flexible ligand clustering method. PDB-Ligand will be a good resource for both a better interpretation of ligand-binding structures and the development of better scoring functions to be used in many drug discovery applications

MOBICORS-Movie: A MOBIle COntents Recommender System for Movie

In spite of the rapid growth of mobile multimedia contents market, most of the customers experience inconvenience, lengthy search processes and frustration in searching for the specific multimedia contents they want. These difficulties are attributable to the current mobile Internet service method based on inefficient sequential search. To overcome these difficulties, this paper proposes a MOBIle COntents Recommender System for Movie (MOBICORS-Movie), which is designed to reduce customers’ search efforts in finding desired movies on the mobile Internet. MOBICORS-Movie consists of three agents: CF (Collaborative Filtering), CBIR (Content-Based Information Retrieval) and RF (Relevance Feedback). These agents collaborate each other to support a customer in finding a desired movie by generating personalized recommendations of movies. To verify the performance of MOBICORS-Movie, the simulation-based experiments were conducted. The experiment results show that MOBICORS-Movie significantly reduces the customer’s search effort and can be a realistic solution for movie recommendation in the mobile Internet environment

Mth1 regulates the interaction between the Rgt1 repressor and the Ssn6-Tup1 corepressor complex by modulating PKA-dependent phosphorylation of Rgt1

Glucose uptake, the first, rate-limiting step of its utilization, is facilitated by glucose transporters. Expression of several glucose transporter (HXT) genes in yeast is repressed by the Rgt1 repressor, which recruits the glucose-responsive transcription factor Mth1 and the general corepressor complex Ssn6-Tup1 in the absence of glucose; however, it is derepressed when Mth1 is inactivated by glucose. Here we show that Ssn6-Tup1 interferes with the DNA-binding ability of Rgt1 in the absence of Mth1 and that the Rgt1 function abrogated by Ssn6 overexpression is restored by co-overexpression of Mth1. Thus Mth1 likely regulates Rgt1 function not by modulating its DNA-binding activity directly but by functionally antagonizing Ssn6-Tup1. Mth1 does so by acting as a scaffold-like protein to recruit Ssn6-Tup1 to Rgt1. Supporting evidence shows that Mth1 blocks the protein kinase A–dependent phosphorylation of Rgt1 that impairs the ability of Rgt1 to interact with Ssn6-Tup1. Of note, Rgt1 can bind DNA in the absence of Ssn6-Tup1 but does not inhibit transcription, suggesting that dissociation of Rgt1 from Ssn6-Tup1, but not from DNA, is necessary and sufficient for the expression of its target genes. Taken together, these findings show that Mth1 is a transcriptional corepressor that facilitates the recruitment of Ssn6-Tup1 by Rgt1