Homogeneous and heterogeneous nucleation in the three--state Blume--Capel model

The metastable behavior of the stochastic Blume--Capel model with Glauber dynamics is studied when zero-boundary conditions are considered. The presence of zero-boundary conditions changes drastically the metastability scenarios of the model: \emph{heterogeneous nucleation} will be proven in the region of the parameter space where the chemical potential is larger than the external magnetic field.Comment: 25 pages, 18 figure

Are Rapid Population Estimates Accurate? A Field Trial of Two Different Assessment Methods.

Emergencies resulting in large-scale displacement often lead to populations resettling in areas where basic health services and sanitation are unavailable. To plan relief-related activities quickly, rapid population size estimates are needed. The currently recommended Quadrat method estimates total population by extrapolating the average population size living in square blocks of known area to the total site surface. An alternative approach, the T-Square, provides a population estimate based on analysis of the spatial distribution of housing units taken throughout a site. We field tested both methods and validated the results against a census in Esturro Bairro, Beira, Mozambique. Compared to the census (population: 9,479), the T-Square yielded a better population estimate (9,523) than the Quadrat method (7,681; 95% confidence interval: 6,160-9,201), but was more difficult for field survey teams to implement. Although applicable only to similar sites, several general conclusions can be drawn for emergency planning

Coevolutionary Landscape Inference and the Context-Dependence of Mutations in Beta-Lactamase TEM-1

The quantitative characterization of mutational landscapes is a task of outstanding importance in evolutionary and medical biology: It is, for example, of central importance for our understanding of the phenotypic effect of mutations related to disease and antibiotic drug resistance. Here we develop a novel inference scheme for mutational landscapes, which is based on the statistical analysis of large alignments of homologs of the protein of interest. Our method is able to capture epistatic couplings between residues, and therefore to assess the dependence of mutational effects on the sequence context where they appear. Compared with recent large-scale mutagenesis data of the beta-lactamase TEM-1, a protein providing resistance against beta-lactam antibiotics, our method leads to an increase of about 40% in explicative power as compared with approaches neglecting epistasis. We find that the informative sequence context extends to residues at native distances of about 20 Å from the mutated site, reaching thus far beyond residues in direct physical contact

The antimicrobial peptide TAT-RasGAP_317-326 inhibits the formation and expansion of bacterial biofilms in vitro.

Biofilms are structured aggregates of bacteria embedded in a self-produced matrix that develop in diverse ecological niches. Pathogenic bacteria can form biofilms on surfaces and in tissues, causing nosocomial and chronic infections that are difficult to treat. While antibiotics are largely inefficient in limiting biofilm formation and expansion, antimicrobial peptides (AMPs) are emerging as alternative antibiofilm treatments. In this study, we explore the effect of the newly described AMP TAT-RasGAP <sub>317-326</sub> on Acinetobacter baumannii, Pseudomonas aeruginosa and Staphylococcus aureus biofilms. Efficiency of TAT-RasGAP <sub>317-326</sub> on biofilms was tested in vitro. Both viability of bacteria contained in the biofilm as well as biomass of the biofilm were quantified using resazurin and crystal violet staining, respectively. The antibiofilm effect of TAT-RasGAP <sub>317-326</sub> was compared with a selection of classical antibiotics and AMPs. We observe that TAT-RasGAP <sub>317-326</sub> inhibits biofilm formation at concentrations equivalent or two times greater than the minimum inhibitory concentration (MIC) of planktonic bacteria. Moreover, TAT-RasGAP <sub>317-326</sub> limits the expansion of A. baumannii and P. aeruginosa established biofilms at twice the concentration inhibiting biofilm formation. These results underscore the potential use of TAT-RasGAP <sub>317-326</sub> against biofilms and encourage further studies in the development of AMPs to treat biofilm-related infections

Transient sex-related changes in the mice hypothalamo–pituitary–adrenal (HPA) axis during the acute phase of the inflammatory process

The potential role of endogenous sex hormones in regulating hypothalamo–pituitary–adrenal (HPA) axis function was investigated after a single injection of endotoxin in adult (8 week old) BALB/c mice of both sexes. The effect of LPS on plasma ACTH, corticosterone (B), testosterone and oestradiol (E) levels and on anterior pituitary (AP) ACTH and adrenal B contents at different times after treatment was studied. The results indicate that: (a) basal B but not ACTH plasma levels were significantly higher in female than in male mice; (b) LPS significantly increased both ACTH and B plasma levels over the baseline 2 h after injection, both hormone levels being higher in female than in male mice; (c) although plasma ACTH concentrations recovered the basal value at 72 h after LPS in animals of both sexes, plasma B levels returned to the baseline only at 120 h after treatment; (d) E plasma levels significantly increased 2 h after LPS and returned to the baseline at 72 h post-treatment, in both sexes; (e) at 2 h after LPS, testosterone plasma levels significantly decreased in male mice and increased in female mice, recovering the baseline level at 120 and 72 h after LPS, respectively; (f) AP ACTH content was similar in both sexes in basal condition and it was significantly diminished 72 h post-treatment without sex difference; whereas AP ACTH returned to basal content 120 h after LPS in males, it remained significantly decreased in females; (g) basal adrenal B content was higher in female than in male mice, and it significantly increased in both sexes 2 h post-LPS, maintaining this sex difference. Whereas adrenal B returned to basal content 72 h after treatment in male mice, it remained significantly enhanced up to 120 h post-LPS in female animals. The data demonstrate the existence of a clear sexual dimorphism in basal condition and during the acute phase response as well as in the recovery of the HPA axis function shortly after infection

A new Manifestation of Atomic Parity Violation in Cesium: a Chiral Optical Gain induced by linearly polarized 6S-7S Excitation

We have detected, by using stimulated emission, an Atomic Parity Violation (APV) in the form of a chiral optical gain of a cesium vapor on the 7S - 6P$_{3/2}$ transition,consecutive to linearly polarized 6S-7S excitation. We demonstrate the validity of this detection method of APV, by presenting a 9% accurate measurement of expected sign and magnitude. We underline several advantages of this entirely new approach in which the cylindrical symmetry of the set-up can be fully exploited. Future measurements at the percent level will provide an important cross-check of an existing more precise result obtained by a different method.Comment: 4 pages, 2 figure

Search for anisotropic effects of hcp solid helium on optical lines of cesium impurities

The anisotropic effect of a hcp 4He solid matrix on cesium atoms has been proposed as a tool to reveal the parity violating anapole moment of its nucleus. It should also result in splitting the D2 optical excitation line in a way depending on the light polarization. An experimental investigation has been set up using oriented hcp helium crystals in which cesium metal grains are embedded. Atoms are created by laser sputtering from this grains. Optical absorption spectra of the D2 line have been recorded in the temperature range of 1.0 to 1.4 K at liquid/solid coexistence pressure by monitoring the fluorescence on the D2 line at 950 nm. No significant effect of the light polarization has been found, suggesting a statistically isotropic disordered solid environment for the cesium atoms.Comment: The original publication will be available at http://www.springerlink.co

Homogeneous and heterogeneous nucleation in the three--state Blume--Capel model

The metastable behavior of the stochastic Blume--Capel model with Glauber dynamics is studied when zero-boundary conditions are considered. The presence of zero-boundary conditions changes drastically the metastability scenarios of the model: \emph{heterogeneous nucleation} will be proven in the region of the parameter space where the chemical potential is larger than the external magnetic field

The Anticancer Peptide TAT-RasGAP317-326 Exerts Broad Antimicrobial Activity.

Antibiotic resistance has become a major health issue. Nosocomial infections and the prevalence of resistant pathogenic bacterial strains are rising steadily. Therefore, there is an urgent need to develop new classes of antibiotics effective on multi-resistant nosocomial pathogenic bacteria. We have previously shown that a cell-permeable peptide derived from the p120 Ras GTPase-activating protein (RasGAP), called TAT-RasGAP317-326, induces cancer cell death, inhibits metastatic progression, and sensitizes tumor cells to various anti-cancer treatments in vitro and in vivo. We here report that TAT-RasGAP317-326 also possesses antimicrobial activity. In vitro, TAT-RasGAP317-326, but not mutated or truncated forms of the peptide, efficiently killed a series of bacteria including Escherichia coli, Acinetobacter baumannii, Staphylococcus aureus, and Pseudomonas aeruginosa. In vivo experiments revealed that TAT-RasGAP317-326 protects mice from lethal E. coli-induced peritonitis if administrated locally at the onset of infection. However, the protective effect was lost when treatment was delayed, likely due to rapid clearance and inadequate biodistribution of the peptide. Peptide modifications might overcome these shortcomings to increase the in vivo efficacy of the compound in the context of the currently limited antimicrobial options