Determination of the glycoforms of human chorionic gonadotropin b-core fragment by matrix-assisted laser desoption/ionisation time of flight mass spectrometry

Background: Metabolism of human chorionic gonadotropin (hCG) in the serum and kidney yields the terminal urinary product hCG ß-core fragment (hCGßcf), comprising two disulfide-linked peptides (ß6-ß40 and ß55-ß92) of which one (ß6-ß40) retains truncated N-linked sugars. Hyperglycosylated hCGßcf may indicate choriocarcinoma or Down syndrome, but the glycosylation profile of hCGßcf has not been thoroughly evaluated. Methods: hCGßcf, purified from pregnancy urine, was reduced by "on-target" dithiothreitol (DTT) reduction and analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The mass ([M+H]+) of the primary sequence of the glycosylated peptide ß6-ß40 was subtracted from the m/z values of the discrete peaks observed to give the masses of the carbohydrate moieties. Carbohydrate structure was predicted by sequentially subtracting the masses of the monosaccharide residues corresponding to N-linked carbohydrates of the hCG ß-subunit reported in the literature. Results: Mass spectra of hCGßcf revealed a broad triple peak at m/z 8700–11300. After reduction, the triple peak was replaced by a discrete set of peaks between m/z 4156 and 6354. A peak at m/z 4156.8 corresponded to the nonglycosylated peptide (ß55-ß92). The remaining nine peaks indicated that urinary hCGßcf comprises a set of glycoforms smaller and larger than the trimannosyl core. Conclusions: hCGßcf comprises a wider set of glycoforms than reported previously. Peaks of highest mass indicate evidence of hyperglycosylated carbohydrate moieties. The data support previous reports that hCGßcf oligosaccharides lack sialic acid and galactose residues. No indication was found of a ß6-ß40 peptide that was entirely devoid of carbohydrate

Is the scaling of swim speed in sharks driven by metabolism?

The movement rates of sharks are intrinsically linked to foraging ecology, predator–prey dynamics and wider ecosystem functioning in marine systems. During ram ventilation, however, shark movement rates are linked not only to ecological parameters, but also to physiology, as minimum speeds are required to provide sufficient water flow across the gills to maintain metabolism. We develop a geometric model predicting a positive scaling relationship between swim speeds in relation to body size and ultimately shark metabolism, taking into account estimates for the scaling of gill dimensions. Empirical data from 64 studies (26 species) were compiled to test our model while controlling for the influence of phylogenetic similarity between related species. Our model predictions were found to closely resemble the observed relationships from tracked sharks, providing a means to infer mobility in particularly intractable species

Calibrating Type Ia Supernovae using the Planetary Nebula Luminosity Function I. Initial Results

We report the results of an [O III] lambda 5007 survey for planetary nebulae (PN) in five galaxies that were hosts of well-observed Type Ia supernovae: NGC 524, NGC 1316, NGC 1380, NGC 1448 and NGC 4526. The goals of this survey are to better quantify the zero-point of the maximum magnitude versus decline rate relation for supernovae Type Ia and to validate the insensitivity of Type Ia luminosity to parent stellar population using the host galaxy Hubble type as a surrogate. We detected a total of 45 planetary nebulae candidates in NGC 1316, 44 candidates in NGC 1380, and 94 candidates in NGC 4526. From these data, and the empirical planetary nebula luminosity function (PNLF), we derive distances of 17.9 +0.8/-0.9 Mpc, 16.1 +0.8/-1.1 Mpc, and 13.6 +1.3/-1.2 Mpc respectively. Our derived distance to NGC 4526 has a lower precision due to the likely presence of Virgo intracluster planetary nebulae in the foreground of this galaxy. In NGC 524 and NGC 1448 we detected no planetary nebulae candidates down to the limiting magnitudes of our observations. We present a formalism for setting realistic distance limits in these two cases, and derive robust lower limits of 20.9 Mpc and 15.8 Mpc, respectively. After combining these results with other distances from the PNLF, Cepheid, and Surface Brightness Fluctuations distance indicators, we calibrate the optical and near-infrared relations for supernovae Type Ia and we find that the Hubble constants derived from each of the three methods are broadly consistent, implying that the properties of supernovae Type Ia do not vary drastically as a function of stellar population. We determine a preliminary Hubble constant of H_0 = 77 +/- 3 (random) +/- 5 (systematic) km/s/Mpc for the PNLF, though more nearby galaxies with high-quality observations are clearly needed.Comment: 25 pages, 12 figures. Accepted for publication by the Astrophysical Journal. Figures degraded to comply with limit. Full paper is available at: http://www.as.ysu.edu/~jjfeldme/pnlf_Ia.pd

Computer Evaluation of Real-Time X-Ray and Acoustic Images

The weakest link in the inspection process is the subjective interpretation of data by inspectors. To overcome this troublesome fact computer based analysis systems have been developed. In the field of nondestructive evaluation (NDE) there is a large class of inspections that can benefit from computer analysis. X-ray images (both film and fluoroscopic) and acoustic images lend themselves to automatic analysis as do the one-dimensional signals associated with ultrasonic, eddy current and acoustic emission testing. Computer analysis can enhance and evaluate subtle details. Flaws can be located and measured, and acceptance decisions made by computer in a consistent and objective manner. This paper describes the interactive, computer-based analysis of real-time x-ray images and acoustic images of graphite/epoxy adhesively bonded structures

Measurement of Orbital Decay in the Double Neutron Star Binary PSR B2127+11C

We report the direct measurement of orbital period decay in the double neutron star pulsar system PSR B2127+11C in the globular cluster M15 at the rate of $(-3.95 \pm 0.13) \times 10^{-12}$, consistent with the prediction of general relativity at the $\sim 3$ level. We find the pulsar mass to be $m_p = (1.358 \pm 0.010) M_\odot$ and the companion mass $m_c = (1.354 \pm 0.010) M_\odot$. We also report long-term pulse timing results for the pulsars PSR B2127+11A and PSR B2127+11B, including confirmation of the cluster proper motion.Comment: 12 pages, 4 figures, accepted for publication in ApJ

Discovery of 9 Ly alpha emitters at redshift z~3.1 using narrow-band imaging and VLT spectroscopy

Narrow-band imaging surveys aimed at detecting the faint emission from the 5007 [O III] line of intracluster planetary nebulae in Virgo also probe high redshift z=3.1 Ly alpha emitters. Here we report on the spectroscopic identification of 9 Ly alpha emitters at z=3.13, obtained with the FORS spectrograph at Unit 1 of the ESO Very Large Telescope (VLT UT1). The spectra of these high redshift objects show a narrow, isolated Ly alpha emission with very faint (frequently undetected) continuum, indicating a large equivalent width. No other features are visible in our spectra. Our Ly alpha emitters are quite similar to those found by Hu (1998), Cowie & Hu (1998) and Hu et al. (1998). Using simple population synthesis models, on the assumption that these sources are regions of star formation, we conclude that the nebulae are nearly optically thick and must have a very low dust content, in order to explain the high observed Ly alpha equivalent widths. For the cosmological and star formation parameters we adopted, the total stellar mass produced would seem to correspond to the formation of rather small galaxies, some of which are perhaps destined to merge. The implied star formation density in our sampled comoving volume is probably somewhat smaller than, but of the same order of magnitude as the star formation density at z=3 derived by other authors from Lyman-break galaxy surveys. This result agrees with the expectation that the Ly alpha emitters are a low-metallicity (or low-dust) tail in a distribution of star forming regions at high redshifts. Finally, the Ly alpha emitters may contribute as many H-ionizing photons as QSOs at z=3.Comment: 26 pages, 17 Postscript figures, ApJ in pres

Early infant feeding and adiposity risk: from infancy to adulthood

Introduction: Systematic reviews suggest that a longer duration of breast-feeding is associated with a reduction in the risk of later overweight and obesity. Most studies examining breast-feeding in relation to adiposity have not used longitudinal analysis. In our study, we aimed to examine early infant feeding and adiposity risk in a longitudinal cohort from birth to young adulthood using new as well as published data. Methods: Data from the Western Australian Pregnancy Cohort (Raine) Study in Perth, W.A., Australia, were used to examine associations between breast-feeding and measures of adiposity at 1, 2, 3, 6, 8, 10, 14, 17, and 20 years. Results: Breast-feeding was measured in a number of ways. Longer breast-feeding (in months) was associated with reductions in weight z-scores between birth and 1 year (β = -0.027; p \u3c 0.001) in the adjusted analysis. At 3 years, breast-feeding for \u3c4 months increased the odds of infants experiencing early rapid growth (OR 2.05; 95% CI 1.43-2.94; p \u3c 0.001). From 1 to 8 years, children breast-fed for ≤4 months compared to ≥12 months had a significantly greater probability of exceeding the 95th percentile of weight. The age at which breast-feeding was stopped and a milk other than breast milk was introduced (introduction of formula milk) played a significant role in the trajectory of the BMI from birth to 14 years; the 4-month cutoff point was consistently associated with a higher BMI trajectory. Introduction of a milk other than breast milk before 6 months compared to at 6 months or later was a risk factor for being overweight or obese at 20 years of age (OR 1.47; 95% CI 1.12-1.93; p = 0.005). Discussion: Breast-feeding until 6 months of age and beyond should be encouraged and is recommended for protection against increased adiposity in childhood, adolescence, and young adulthood. Adverse long-term effects of early growth acceleration are fundamental in later overweight and obesity. Formula feeding stimulates a higher postnatal growth velocity, whereas breast-feeding promotes slower growth and a reduced likelihood of overweight and obesity. Biological mechanisms underlying the protective effect of breast-feeding against obesity are based on the unique composition and metabolic and physiological responses to human milk

Optical Detection of Two Intermediate Mass Binary Pulsar Companions

We report the detection of probable optical counterparts for two Intermediate Mass Binary Pulsar (IMBP) systems, PSR J1528-3146 and PSR J1757-5322. Recent radio pulsar surveys have uncovered a handful of these systems with putative massive white dwarf companions, thought to have an evolutionary history different from that of the more numerous class of Low Mass Binary Pulsars (LMBPs) with He white dwarf companions. The study of IMBP companions via optical observations offers us several new diagnostics: the evolution of main sequence stars near the white-dwarf-neutron star boundary, the physics of white dwarfs close to the Chandrasekhar limit, and insights into the recycling process by which old pulsars are spun up to high rotation frequencies. We were unsuccessful in our attempt to detect optical counterparts of PSR J1141-6545, PSR J1157-5112, PSR J1435-6100, and PSR J1454-5846.Comment: 9 pages, 2 figures, accepted for publication in ApJ

Evidence for a Massive Neutron Star from a Radial-Velocity Study of the Companion to the Black Widow Pulsar PSR B1957+20

The most massive neutron stars constrain the behavior of ultra-dense matter, with larger masses possible only for increasingly stiff equations of state. Here, we present evidence that the black widow pulsar, PSR B1957+20, has a high mass. We took spectra of its strongly irradiated companion and found an observed radial-velocity amplitude of K_obs=324+/-3 km/s. Correcting this for the fact that, due to the irradiation, the center of light lies inward relative to the center of mass, we infer a true radial-velocity amplitude of K_2=353+/-4 km/s and a mass ratio q=M_PSR/M_2=69.2+/-0.8. Combined with the inclination i=65+/-2 deg inferred from models of the lightcurve, our best-fit pulsar mass is M_PSR=2.40+/-0.12 M_sun. We discuss possible systematic uncertainties, in particular in the lightcurve modeling. Taking an upper limit of i<85 deg based on the absence of radio eclipses at high frequency, combined with a conservative lower-limit to the motion of the center of mass, K_2>343 km/s (q>67.3), we infer a lower limit to the pulsar mass of M_PSR>1.66 M_sun.Comment: 7 pages, 3 figures, 1 table, accepted for publication in ApJ; revision includes more detail on the spectral classification and discussion of other recent high neutron-star masse