832 research outputs found
Preferential Binding Effects On Protein Structure and Dynamics Revealed by Coarse-Grained Monte Carlo Simulation
The effect of preferential binding of solute molecules within an aqueous solution on the structure and dynamics of the histone H3.1 protein is examined by a coarse-grained Monte Carlo simulation. The knowledge-based residue-residue and hydropathy-index-based residue-solvent interactions are used as input to analyze a number of local and global physical quantities as a function of the residue-solvent interaction strength (f). Results from simulations that treat the aqueous solution as a homogeneous effective solvent medium are compared to when positional fluctuations of the solute molecules are explicitly considered. While the radius of gyration (Rg) of the protein exhibits a non-monotonic dependence on solvent interaction over a wide range of f within an effective medium, an abrupt collapse in Rg occurs in a narrow range of f when solute molecules rapidly bind to a preferential set of sites on the protein. The structure factor S(q) of the protein with wave vector (q) becomes oscillatory in the collapsed state, which reflects segmental correlations caused by spatial fluctuations in solute-protein binding. Spatial fluctuations in solute binding also modify the effective dimension (D) of the protein in fibrous (D ∼ 1.3), random-coil (D ∼ 1.75), and globular (D ∼ 3) conformational ensembles as the interaction strength increases, which differ from an effective medium with respect to the magnitude of D and the length scale
Analyzing Powers for Deuteron-Induced Reactions Leading to Continuum Final States
This work was supported by the National Science Foundation Grant NSF PHY 78-22774 A02 & A03 and by Indiana Universit
Reaction Mechanism Implications of Deuteron Rainbow Scattering
This work was supported by the National Science Foundation Grant NSF PHY 78-22774 A02 & A03 and by Indiana Universit
Measurements of X_2 and Other Results in the Analysis of d + 58-Ni Elastic Scattering
This work was supported by the National Science Foundation Grants NSF PHY 78-22774 A03, NSF PHY 81-14339, and by Indiana Universit
Systematics of the K X-Ray Multiplicity for Transitional Nuclei with A = 200
This work was supported by the National Science Foundation Grants NSF PHY 78-22774 A03, NSF PHY 81-14339, and by Indiana Universit
Features of the Analyzing Powers in Deuteron Elastic Scattering Near 80 MeV
This work was supported by the National Science Foundation Grant NSF PHY 78-22774 A02 & A03 and by Indiana Universit
Optical Model Analysis of Elastic Deuteron Scattering at 80 MeV
This research was sponsored by the National Science Foundation Grant NSF PHY 87-1440
Fermi liquid interactions and the superfluid density in d-wave superconductors
We construct a phenomenological superfluid Fermi liquid theory for a
two-dimensional d-wave superconductor on a square lattice, and study the effect
of quasiparticle interactions on the superfluid density. Using simple models
for the dispersion and the Landau interaction function, we illustrate the
deviation of these results from those for the isotropic superfluid. This allows
us to reconcile the value and doping dependence of the superfluid density slope
at low temperature obtained from penetration depth measurements, with
photoemission data on nodal quasiparticles.Comment: 5 latex pages, 1 eps-figure. submitted to PR
Herpes simplex virus 1 amplicon vector-mediated siRNA targeting epidermal growth factor receptor inhibits growth of human glioma cells in vivo
In primary glioblastomas and other tumor types, the epidermal growth factor receptor (EGFR) is frequently observed with alterations, such as amplification, structural rearrangements, or overexpression of the gene, suggesting an important role in glial tumorigenesis and progression. In this study, we investigated whether posttranscriptional gene silencing by vector-mediated RNAi to inhibit EGFR expression can reduce the growth of cultured human gli36 glioma cells. To "knock down" EGFR expression, we have created HSV-1-based amplicons that contain the RNA polymerase III-dependent H1 promoter to express double-stranded hairpin RNA directed against EGFR at two different locations (pHSVsiEGFR I and pHSVsiEGFR II). We demonstrate that both pHSVsiEGFR I and pHSVsiEGFR II mediated knock-down of transiently transfected full-length EGFR or endogenous EGFR in a dose-dependent manner. The knock-down of EGFR resulted in the growth inhibition of human glioblastoma (gli36-luc) cells both in culture and in athymic mice in vivo. Cell cycle analysis and annexin V staining revealed that siRNA-mediated suppression of EGFR induced apoptosis. Overall HSV-1 amplicons can mediate efficient and specific posttranscriptional gene silencing. Copyright © The American Society of Gene Therapy
4pi Models of CMEs and ICMEs
Coronal mass ejections (CMEs), which dynamically connect the solar surface to
the far reaches of interplanetary space, represent a major anifestation of
solar activity. They are not only of principal interest but also play a pivotal
role in the context of space weather predictions. The steady improvement of
both numerical methods and computational resources during recent years has
allowed for the creation of increasingly realistic models of interplanetary
CMEs (ICMEs), which can now be compared to high-quality observational data from
various space-bound missions. This review discusses existing models of CMEs,
characterizing them by scientific aim and scope, CME initiation method, and
physical effects included, thereby stressing the importance of fully 3-D
('4pi') spatial coverage.Comment: 14 pages plus references. Comments welcome. Accepted for publication
in Solar Physics (SUN-360 topical issue
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