Baker Center Journal of Applied Public Policy - Vol. IV, No.II

This special edition includes articles from speakers at a 2010 conference - Howard H. Baker, Jr: A Life in Public Service and a special addendum including photographs and cartoons from Sen. Baker\u27s career

Use of Co-Products from the Processing of Cassava for the Development of Adsorbent Materials Aiming Metal Removal

Nowadays the contamination of water resources by the most varied pollutants have been accelerated. Technologies of decontamination of water are too costly, however, the development of low cost adsorbents, have proven to be efficient, promising and cheap alternatives for this purpose. The use of adsorbents from cassava residues has shown great potential for use as an adsorbent. The productive chain of this crop involves the production and processing of its roots, generating a large volume of solid waste. Aiming the sustainability of production systems, productive chains should optimize the production of cassava barks residues, since these do not present significant uses or benefit. In this scenario, this chapter gathers information from the literature on the use of solid waste from the cassava agroindustry and its use as adsorbents, aiming the removal of toxic metals, as well as their potential for the treatment of other contaminants. Several authors have demonstrated through studies the potentiality of the use of agroindustrial cassava residues as adsorbents. Because of a range of characteristics observed these adsorbents present viability for large-scale use, being in very similar to activated carbon. Thus, the use of these adsorbent materials represents an extremely viable and sustainable alternative

Structural and Molecular Analysis of a Protective Epitope of Lyme Disease Antigen OspA and Antibody Interactions

The murine monoclonal antibody LA-2 recognizes a clinically protective epitope on outer surface protein (OspA) of Borrelia burgdorferi, the causative agent of Lyme disease in North America. Human antibody equivalence to LA-2 is the best serologic correlate of protective antibody responses following OspA vaccination. Understanding the structural and functional basis of the LA-2 protective epitope is important for developing OspA-based vaccines and discovering prophylactic antibodies against Lyme disease. Here, we present a detailed structure-based analysis of the LA-2/OspA interaction interface and identification of residues mediating antibody recognition. Mutations were introduced into both OspA and LA-2 based on computational predictions on the crystal structure of the complex, and experimentally tested for in-vitro binding and borreliacidal activity. We find that Y32 and H49 on the LA-2 light chain, N52 on the LA-2 heavy chain and residues A208, N228 and N251 on OspA were the key constituents of OspA/LA-2 interface. These results reveal specific residues that may be exploited to modulate recognition of the protective epitope of OspA and have implications for design of vaccines against Lyme disease

Scaling and criticality of the Kondo effect in a Luttinger liquid

A quantum Monte Carlo simulation method has been developed and applied to study the critical behavior of a single Kondo impurity in a Luttinger liquid. This numerically exact method has no finite-size limitations and allows to simulate the whole temperature range. Focusing on the impurity magnetic susceptibility, we determine the scaling functions, in particular for temperatures well below the Kondo temperature. In the absence of elastic potential scattering, we find Fermi-liquid behavior for strong electron-electron interactions, g_c < 1/2, and anomalous power laws for 1/2<g_c < 1, where g_c is the correlation parameter of the Luttinger liquid. These findings resolve a recent controversy. If elastic potential scattering is present, we find a logarithmically divergent impurity susceptibility at g_c<1/2 which can be rationalized in terms of the two-channel Kondo model.Comment: 11 pages REVTeX, incl. 9 PS figures, subm. to PR

Identification and Characterization of Human Monoclonal Antibodies for Immunoprophylaxis Against Enterotoxigenic Escherichia coli Infection

Background. Enterotoxigenic Escherichia coli (ETEC) cause diarrheal illness in infants in the developing world and travelers to endemic countries including military personnel. ETEC infection of the host involves colonization of the small intestinal epithelium and toxin secretion leading to watery diarrhea. There is currently no vaccine licensed to prevent ETEC. CFA/I is one of the most common colonization factor antigens (CFAs). The CFA/I adhesin subunit, CfaE, is required for ETEC adhesion to host intestinal cells. Human antibodies against CfaE have potential to block colonization of ETEC and serve as an immunoprophylactic against ETEC-related diarrhea. Methods. Mice transgenic for human immunoglobulin genes were immunized with CfaE to generate a panel of human monoclonal IgG1 antibodies (HuMAbs). The most potent IgG1 identified in the in vitro functional assays were selected and isotype switched to secretory IgA (sIgA) and tested in animal colonization assays via oral administration. Results. Over 300 unique anti-CfaE IgG1 HuMabs were identified. The lead IgG1 anti-CfaE HuMAbs completely inhibited hemagglutination and blocked adhesion of ETEC to Caco-2 cells. Epitope mapping studies revealed that HuMAbs recognized epitopes in the N-terminal domain of CfaE near the putative receptor binding site. Oral administration of anti-CfaE antibodies in either IgG or secretory IgA isotypes inhibited intestinal colonization in mice challenged with ETEC. A two to four log decrease of colony forming units was observed as compared to irrelevant isotype controls. Conclusions. We identified fully human monoclonal antibodies against CfaE adhesion domain that can be potentially employed as an immunoprophylaxis to prevent ETEC-related diarrhea

Bounds on Integrals of the Wigner Function

The integral of the Wigner function over a subregion of the phase-space of a quantum system may be less than zero or greater than one. It is shown that for systems with one degree of freedom, the problem of determining the best possible upper and lower bounds on such an integral, over all possible states, reduces to the problem of finding the greatest and least eigenvalues of an hermitian operator corresponding to the subregion. The problem is solved exactly in the case of an arbitrary elliptical region. These bounds provide checks on experimentally measured quasiprobability distributions.Comment: 10 pages, 1 PostScript figure, Latex file; revised following referees' comments; to appear in Physical Review Letter