Movement of Translocated Adult Sicklefin Redhorse (\u3ci\u3eMoxostoma\u3c/i\u3e sp.) in the Oconaluftee River, North Carolina: Implications for Species Restoration

The Sicklefin Redhorse is a rare, undescribed species of Moxostoma, endemic to the Hiwassee and Little Tennessee River basins of western North Carolina and northern Georgia, where it has been eliminated from much of its native range. It is listed as endangered in Georgia and threatened in North Carolina. Although it has not been granted federal protected status, this species is the subject of a Candidate Conservation Agreement between federal, state, tribal, and private stakeholders, of which one objective calls for the re-establishment of Sicklefin Redhorse populations throughout its historical range. The objective of our study was to evaluate suitability of North Carolina’s upper Oconaluftee River for reintroduction of Sicklefin Redhorse, by tracking movement patterns of translocated individuals. Ten native Sicklefin Redhorse were collected from the Tuckasegee River in Swain County, NC, implanted with radio transmitters and translocated into the Oconaluftee River upstream from Ela Dam. Fish were tracked individually using radio telemetry for six months. Movement patterns for newly translocated fish, as well as seasonal patterns for females, were comparable to those shown in previous studies within the current range of Sicklefin Redhorse. Although some fish moved extensively, the sedentary patterns observed in females suggests that the upper Oconaluftee River may provide suitable overwinter habitat for the Sicklefin Redhorse. However, additional data are needed concerning spawning suitability and rates of downstream migration past Ela Dam before reintroducing Sicklefin Redhorse back to this portion of its native range

Effect of Remote Ischaemic Conditioning on the inflammatory cytokine cascade of COVID-19 (RIC in COVID-19): a Randomized Controlled Trial

© The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.Purpose Patients hospitalized with COVID-19 may develop a hyperinflammatory, dysregulated cytokine “storm” that rapidly progresses to acute respiratory distress syndrome, multiple organ dysfunction, and even death. Remote ischaemic conditioning (RIC) has elicited anti-inflammatory and cytoprotective benefits by reducing cytokines following sepsis in animal studies. Therefore, we investigated whether RIC would mitigate the inflammatory cytokine cascade induced by COVID-19. Methods We conducted a prospective, multicentre, randomized, sham-controlled, single-blind trial in Brazil and South Africa. Non-critically ill adult patients with COVID-19 pneumonia were randomly allocated (1:1) to receive either RIC (intermittent ischaemia/reperfusion applied through four 5-min cycles of inflation (20 mmHg above systolic blood pressure) and deflation of an automated blood-pressure cuff) or sham for approximately 15 days. Serum was collected following RIC/sham administration and analyzed for inflammatory cytokines using flow cytometry. The endpoint was the change in serum cytokine concentrations. Participants were followed for 30 days. Results Eighty randomized participants (40 RIC and 40 sham) completed the trial. Baseline characteristics according to trial intervention were overall balanced. Despite downward trajectories of all cytokines across hospitalization, we observed no substantial changes in cytokine concentrations after successive days of RIC. Time to clinical improvement was similar in both groups (HR 1.66; 95% CI, 0.938–2.948, p 0.08). Overall RIC did not demonstrate a significant impact on the composite outcome of all-cause death or clinical deterioration (HR 1.19; 95% CI, 0.616–2.295, p = 0.61). Conclusion RIC did not reduce the hypercytokinaemia induced by COVID-19 or prevent clinical deterioration to critical care.Peer reviewe

Process Of Extractive Fermentation And Use Thereof

PROCESSO DE FERMENTAÇÃO EXTRATIVA E USO DO MESMO. A presente invenção se refere a um processo de fermentação extrativa. Mais especificamente, a presente invenção se refere a um processo de fermentação extrativa que emprega um único solvente capaz de remover produtos e inibidores simultaneamente, além de resfriar o meio. Além disso, apresente invenção se refere ao uso do processo. Especificamente, a presente invenção se refere ao uso do biodiesel, tais como biodiesel de óleo de soja, de milho, de mamona, de dendezeiro, de macaúba, de macaúba, entre outros, como agente extrator biocompatível para a extração in-situ de produto e/ou componentes inibidores da fermentação, especificamente o produto da fermentação e compostos inibidores presentes nos mostos provenientes de processos hidrolíticos de matéria-prima lingocelulósica, amilácea, carboidratos diretamente extraíveis, melaços fortemente esgotados com alto conteúdo de inibidores como, entre outros, biotina, ou mostos similares ricos em carboidratos, como também para o resfriamento do meio da fermentação pelo próprio solvente.BRPI1105142 (A2)C12P7/06B01D3/34B01D11/04BR2011PI05142C12P7/06B01D3/34B01D11/0