Improvements in the methylmercury extraction from human hair by headspace solid-phase microextraction followed by gas-chromatography cold-vapour atomic fluorescence spectrometry

Improvements in the methylmercury extraction from human hair by solid-phase microextraction followed by gas chromatography coupled to cold-vapour atomic fluorescence spectrometry (GC-CVAFS) have been carried out. They consisted in the optimisation of the digestion step prior to the aqueous-phase ethylation and in the GC-CVAFS interface set-up. The main digestion parameters such as acid type, concentration, temperature and time have been optimised for hair sample analysis, thereby avoiding methylmercury degradation. Moreover, the stability of the digested samples was evaluated to improve the sample throughput

Prevalence and antimicrobial resistance of MRSA across different pig age groups in an intensive pig production system in Australia

This observational study aimed to determine MRSA prevalence using strain‐specific real‐time PCR at the pig level, stratified by age groupings, within a pig enterprise. A total of 658 samples were collected from individual pigs (n = 618) and the piggery environment (n = 40), distributed amongst five different pig age groups. Presumptive MRSA isolates were confirmed by the presence of mecA , and MALDI‐TOF was performed for species verification. All isolates were tested against 18 different antimicrobials. MRSA was isolated from 75.2% (95% CI 71.8–78.6) of samples collected from pigs, and 71% of the MRSA isolates from this source were identified as community‐associated (CA)‐MRSA ST93, while the remainder were livestock‐associated (LA)‐MRSA ST398. Amongst environmental isolates, 80% (CI 64.3–95.7) were ST93 and the remainder ST398. All MRSA isolates from pigs and the environment were susceptible to ciprofloxacin, gentamicin, linezolid, mupirocin, rifampicin, sulfamethoxazole–trimethoprim, teicoplanin and vancomycin. Phenotypic rates of resistance were penicillin (100%), clindamycin (97.6%), erythromycin (96.3%), ceftiofur (93.7%), chloramphenicol (81.2%), tetracycline (63.1%) and amoxicillin–clavulanate (63.9%). A low prevalence of resistance (9.2%) was observed against neomycin and quinupristin–dalfopristin. The probability of MRSA carriage in dry sows (42.2%) was found to be significantly lower (p < .001) when compared to other age groups: farrowing sows (76.8%, RR1.82), weaners (97.8%, RR 2.32), growers (94.2%, RR 2.23) and finishers (98.3%, RR 2.33). Amongst different production age groups, a significant difference was also found in antimicrobial resistance for amoxicillin–clavulanate, neomycin, chloramphenicol and tetracycline. Using the RT‐PCR assay adopted in this study, filtering of highly prevalent ST93 and non‐ST93 isolates was performed at high throughput and low cost. In conclusion, this study found that weaner pigs presented a higher risk for CA‐MRSA and antimicrobial resistance compared to other age groups. These findings have major implications for how investigations of MRSA outbreaks should be approached under the One‐Health context

Reflecting on One Health in Action During the COVID-19 Response

The COVID-19 pandemic, a singular disruptive event in recent human history, has required rapid, innovative, coordinated and collaborative approaches to manage and ameliorate its worst impacts. However, the threat remains, and learning from initial efforts may benefit the response management in the future. One Health approaches to managing health challenges through multi-stakeholder engagement are underscored by an enabling environment. Here we describe three case studies from state (New South Wales, Australia), national (Ireland), and international (sub-Saharan Africa) scales which illustrate different aspects of One Health in action in response to the COVID-19 pandemic. In Ireland, a One Health team was assembled to help parameterise complex mathematical and resource models. In New South Wales, state authorities engaged collaboratively with animal health veterinarians and epidemiologists to leverage disease outbreak knowledge, expertise and technical and support structures for application to the COVID-19 emergency. The African One Health University Network linked members from health institutions and universities from eight countries to provide a virtual platform knowledge exchange on COVID-19 to support the response. Themes common to successful experiences included a shared resource base, interdisciplinary engagement, communication network strategies, and looking global to address local need. The One Health approaches used, particularly shared responsibility and knowledge integration, are benefiting the management of this pandemic and future One Health global challenges

La determinación del concepto de homogeneidad versus resistencia en los pilares de una catedral gótica mediante técnicas no invasivas

The construction and stone-cutting of columns in Gothic construction rules the transmission of the thrusts of the vaults to the ground. The morphologic and typological definition of these elements is essential. In the cathedral of Tortosa was defined an experimental structural model with a double ambulatory, with the construction of the ring of chapels between 1377 and 1424. These chapels are singular since they are not separated by a wall, as uses in Meridional Gothic. The suppression of this wall was after debated in the Cathedral of Milan in 1392. The survey of these columns through noninvasive techniques, such as Terrestrial Laser Scanner and Ground Penetrating Radar has enabled to identify essential differences between them. The study concluded that these columns are homogeneous only in the base and the first three rows of the body and near the impost, which are the areas were concentrates the main transmission of loads.La construcción y talla de los pilares en la construcción gótica son la base de la descarga de los empujes de las bóvedas hacia el terreno. La determinación morfológica y tipológica de estos elementos es esencial. En la catedral de Tortosa se experimenta un modelo estructural con doble deambulatorio, construyendo sus capillas entre 1377 y 1424. Éstas tienen la peculiaridad de no disponer de muro de separación entre ellas, como era frecuente en el gótico meridional. La eliminación del muro fue una cuestión debatida posteriormente en la catedral de Milán en 1392. El diseño y modelización de estos pilares, mediante técnicas no invasivas, utilizando el láser terrestre y el georadar, ha permitido constatar diferencias esenciales en su tipología. Se ha determinado que estos pilares son solamente homogéneos en los puntos de transmisión importantes de las cargas de la fábrica, que son en la basa y las tres primeras hiladas del fuste, así como en la parte superior de la imposta

From Reality to Point Clouds. Survey and Analysis of Sant Miquel Church of Batea (Spain)

Current massive data capture techniques (MDCT), such as terrestrial laser scanning and digital photogrammetry, have become widespread during the last decade, and raises the possibility of developing new assessment methodologies based on the 3D documentation. The paper exposes de results of using simple, non-invasive procedures for the assessment of the shape of a masonry building from a point clouds registered with a terrestrial laser scanner. The case study selected is the church of Sant Miquel of Batea (eighteenth century), located in the province of Tarragona (Spain). The methodology used combines 2D and 3D processes for assessment of the geometry. The results of the investigation have confirmed the good condition of the building. It presents dimensional variations, most of which can be related to the construction process, while some displacements identified provide information about the accommodation process of the masonry structure

La reconstrucción de un palimpsesto románico mediante técnicas no destructivas

The Gothic Cathedral of Tortosa (1346), is superposed over a Romanesque building (1178) as a palimpsest, which there is no physical evidence. The geophysical survey (2102-13), has provided some relevant data from the previous seo, which have enabled the location of some of its main features: apse, pillars of the nave and façade. With these data it is possible to understand the fitting of the process, constructive/ deconstructive of both buildings. The study concludes that the main structural elements of both buildings fit as a gear, and the possible dimensions of the Romanesque Seo are deducted through the evidences found in the subsoil.La catedral gótica de Tortosa (1346), se superpone sobre una construcción románica (1178) a modo de alimpsesto, de la cual no existe ninguna evidencia física. La prospección geofísica (2102-13), ha aportado algunos datos relevantes de la anterior seo, que han precisado la ubicación de alguna de sus partes principales: ábside, pilares de la nave y fachada. Con estos datos se puede entender el encaje del proceso, constructivo/deconstructivo de ambos edificios. El estudio concluye que los elementos estructurales más importantes de ambas edificaciones encajan a manera de engranaje, y se deducen las posibles dimensiones de la Seo románica en base a las evidencias encontradas en el subsuelo

Risk factors for metachronous colorectal cancer or advanced lesions after endoscopic resection of serrated polyps: a systematic review and meta-analysis

\ua9 2024 American Society for Gastrointestinal Endoscopy. Background and Aims: Serrated polyps (SPs) are precursors to 15% to 20% of colorectal cancers (CRCs). However, there are uncertainties regarding which SPs require surveillance and at what intervals, with recommendations adapted from those for adenomas in the absence of solid evidence. Our aim was to assess which SP risk characteristics relate to a higher risk of metachronous CRC or advanced polyps. Methods: We systematically searched PubMed, Embase, and Cochrane for cohort studies, case-control studies, and clinical trials from inception to December 31, 2023, of CRC or advanced polyps (advanced adenoma [AA] or advanced SP) incidence at surveillance stratified by baseline SP size, dysplasia, location, and multiplicity. We defined advanced SPs as those ≥10 mm or with dysplasia. CRC and advanced polyp incidence per 1000 person-years were estimated. We performed a meta-analysis by calculating pooled relative risks (RRs) using a random-effects model. Results: A total of 5903 studies were reviewed, and 14 were included with 493,949 patients (mean age, 59.5 years; 55% men). The mean follow-up was 4.9 years. CRC incidence per 1000 person-years was 2.09 (95% confidence interval [CI], 1.29-2.90) for advanced SPs, 1.52 (95% CI, 0.78-2.25) for SPs of ≥10 mm, 5.86 (95% CI, 2.16-9.56) for SPs with dysplasia, 1.18 (95% CI, 0.77-1.60) for proximal SPs, 0.52 (95% CI, 0.08-1.12) for ≥3 SPs, 0.50 (95% CI, 0.35-0.66) for nonadvanced SPs, and 0.44 (95% CI, 0.41-0.46) for normal colonoscopy findings. Metachronous CRC risk was higher in advanced SPs versus nonadvanced SPs (RR, 1.84; 95% CI, 1.11-3.04) and versus normal colonoscopy findings (RR, 2.92; 95% CI, 2.26-3.77), in SPs of ≥10 mm versus &lt;10 mm (RR, 2.61; 95% CI, 1.43-4.77) and versus normal colonoscopy findings (RR: 3.52; 95% CI, 2.17-5.69); and in SPs with dysplasia versus normal colonoscopy findings (RR: 2.71; 95% CI, 2.00-3.67). No increase in CRC or advanced polyp risk was found in patients with proximal versus distal SPs, nor in ≥3 SPs versus 1 or 2 SPs. Conclusions: CRC risk is significantly higher in patients with baseline advanced SPs after 4.9 years of follow-up, with risk magnitudes similar to those described for AA, supporting the current recommendation for 3-year surveillance in patients with advanced SPs