13 research outputs found
OcorrĂȘncia de flebotomĂneos (Diptera, Psychodidae) em focos de leishmanioses, em ĂĄrea de ecoturismo do entorno do Parque Nacional dos Lençóis Maranhenses, Brasil
Value of diagnostic tools for myocardial ischemia used in routine clinical practice to predict cardiac events in patients with type 2 diabetes mellitus: a prospective study
Population-based prevalence of cervical infection with human papillomavirus genotypes 16 and 18 and other high risk types in Tlaxcala, Mexico
This study was supported by the National Institute of Public Health of
Mexico, the CoordinaciĂłn de InvestigaciĂłn en Salud del Instituto Mexicano
del Seguro Social, the SecretarĂa de Salud Tlaxcala, the Instituto Nacional de
las Mujeres, and the Consejo Nacional de Ciencia y TecnologĂa [FOSISS 2013
202468]. Additional support has been provided by Roche Diagnostics, BD
Diagnostics, DICIPA and Arbor Vita Corporation. The study sponsors did not
played a role in designing the study, collecting, analyzing or interpreting the
data, writing the report, or submitting this paper for publication. UC Berkeley
Center for Global Public Health, Schoeneman Grant, Joint Medical Program
Thesis Grant, and Cancer Research UK (C569/A10404)
P1â025: Cerebral Perfusion as an Imaging Biomarker of Presymptomatic Genetic Frontotemporal Dementia: Preliminary Results from the Genetic Frontotemporal Dementia Initiative (GENFI)
EARLY FUNCTIONAL RESULTS OF BIOFEEDBACK AND ITS IMPACT ON QUALITY OF LIFE OF PATIENTS WITH ANAL INCONTINENCE
Registro de Culicidae de importĂąncia epidemiolĂłgica na ĂĄrea rural de Manaus, Amazonas
DiagnĂłstico molecular da taxa de infecção natural de flebotomĂneos (Psychodidae, Lutzomyia) por Leishmania sp na AmazĂŽnia maranhense Molecular diagnosis of the natural infection rate due to Leishmania sp in sandflies (Psychodidae, Lutzomyia) in the Amazon region of MaranhĂŁo, Brazil
A taxa de infecção natural de trĂȘs diferentes espĂ©cies de flebotomĂneos por Leishmania foi estudada usando a tĂ©cnica de reação em cadeia da polimerase. Primers especĂficos para Leishmania foram designados para examinar se os pools de flebotomĂneos estavam infectadas. Um total de 1.100 fĂȘmeas separadas em pools de 10 indivĂduos foram examinados, consistindo de 50 Lutzomyia whitmani, 43 Lutzomyia triacantha e 17 Lutzomyia choti. De todos os pools analisados, 4 de Lutzomyia whitmani estavam positivos, mas nenhum pool das duas espĂ©cies restantes estava infectado. Deste modo, uma taxa de infecção de 0,4% foi verificada neste estudo. Esta taxa de infecção associada a estudos anteriores sugere que Lutzomyia whitmani transmite Leishmania aos mamĂferos em Buriticupu, MaranhĂŁo.<br>The natural infection rate due to Leishmania was studied in three different sandfly species using the polymerase chain reaction technique. Leishmania specific primers were designed to examine whether sandfly pools were infected. In total 1,100 female sandflies separated into pools of 10 individuals, consisting of 50 pools of Lutzomyia whitmani, 43 of Lutzomyia triacantha and 17 of Lutzomyia choti, were analyzed. Among all the pools examined, four pools of Lutzomyia whitmani were positive, but none of the pools of the other two species were infected. Thus, a total infection rate of 0.4% was established in this study. A similar infection rate was found in previous studies, suggesting that Lutzomyia whitmani transmits Leishmania to mammals in Buriticupu, MaranhĂŁo
In vitro propagation of the wild carrot Daucus carota L. subsp. halophilus (Brot.) A. Pujadas for conservation purposes
Daucus carota subsp. halophilus, is a wild crop
relative of domestic carrot. It is an aromatic plant widely used in folk medicine due to recognized therapeutic properties of its essential oils. Experiments were carried out to evaluate the potential of in vitro propagation techniques to the conservation of this endemic and endangered taxon. The results showed that shoot tips of in vitro germinated seeds were able to proliferate in the presence of benzyladenine, with the best results being
achieved using 4.4ÎŒM, both in the first and second cultures. Shoots rooted after being transferred to 1/2- Murashige and Skoog basal medium. The results indicated that the concentration of benzyladenine used during the
multiplication phase did not interfere with the rate of root formation. The obtained plantlets were morphologically and anatomically identical to those obtained by seeds. Some of
the in vitro produced shoots developed flowers that produced viable pollen. Plant regeneration was also achieved by somatic embryogenesis induction in cotyledons and root segments cultured in the presence of 4.5ÎŒM 2,4-dichlorophenoxyacetic acid. Somatic embryos converted into plantlets in a medium without growth regulators. Plants obtained either by shoot proliferation or somatic
embryogenesis were acclimatized and are now growing at the Coimbra Botanical Garden. The first attempts to reintroduce these plants in the original habitat were successful. It can be concluded that the protocols developed are a useful approach to the conservation of this endemic species